Nitric Oxide Dependent Degradation of Polyethylene Glycol-Modified Single-Walled Carbon Nanotubes: Implications for Intra-Articular Delivery

2018 ◽  
Vol 7 (6) ◽  
pp. 1700916 ◽  
Author(s):  
Kunal Bhattacharya ◽  
Cristiano Sacchetti ◽  
Pedro M. Costa ◽  
Jens Sommertune ◽  
Birgit D. Brandner ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Carbon Nanotubes ◽  
Polyethylene Glycol ◽  
Single Walled Carbon Nanotubes ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

scholarly journals Chemically Functionalized Water-Soluble Single-Walled Carbon Nanotubes Obstruct Vesicular/Plasmalemmal Recycling in Astrocytes Down-Stream of Calcium Ions

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1597
Author(s):  
Manoj K. Gottipati ◽  
Elena Bekyarova ◽  
Robert C. Haddon ◽  
Vladimir Parpura
Keyword(s):  
Carbon Nanotubes ◽  
Plasma Membrane ◽  
Polyethylene Glycol ◽  
Adenosine Triphosphate ◽  
Calcium Ions ◽  
Glutamate Release ◽  
Single Walled Carbon Nanotubes ◽  
Water Soluble ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

We used single-walled carbon nanotubes chemically functionalized with polyethylene glycol (SWCNT-PEG) to assess the effects of this nanomaterial on astrocytic endocytosis and exocytosis. We observed that the SWCNT-PEG do not affect the adenosine triphosphate (ATP)-evoked Ca2+ elevations in astrocytes but significantly reduce the Ca2+-dependent glutamate release. There was a significant decrease in the endocytic load of the recycling dye during constitutive and ATP-evoked recycling. Furthermore, SWCNT-PEG hampered ATP-evoked exocytotic release of the loaded recycling dye. Thus, by functionally obstructing evoked vesicular recycling, SWCNT-PEG reduced glutamate release from astrocytes via regulated exocytosis. These effects implicate SWCNT-PEG as a modulator of Ca2+-dependent exocytosis in astrocytes downstream of Ca2+, likely at the level of vesicle fusion with/pinching off the plasma membrane.

scholarly journals Polyethylene-Glycol-Modified Single-Walled Carbon Nanotubes for Intra-Articular Delivery to Chondrocytes

ACS Nano ◽  
2014 ◽  
Vol 8 (12) ◽  
pp. 12280-12291 ◽  
Author(s):  
Cristiano Sacchetti ◽  
Ru Liu-Bryan ◽  
Andrea Magrini ◽  
Nicola Rosato ◽  
Nunzio Bottini ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Polyethylene Glycol ◽  
Single Walled Carbon Nanotubes ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes
Sign in / Sign up

Export Citation Format

Share Document