Stem Cells: Reconstituting Vascular Microenvironment of Neural Stem Cell Niche in Three-Dimensional Extracellular Matrix (Adv. Healthcare Mater. 9/2014)

2014 ◽  
Vol 3 (9) ◽  
pp. 1349-1349 ◽  
Author(s):  
Yoojin Shin ◽  
Kisuk Yang ◽  
Sewoon Han ◽  
Hyun-Ji Park ◽  
Yun Seok Heo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Three Dimensional ◽  
Neural Stem Cell Niche ◽  
Cell Niche

Reconstituting Vascular Microenvironment of Neural Stem Cell Niche in Three-Dimensional Extracellular Matrix

2014 ◽  
Vol 3 (9) ◽  
pp. 1457-1464 ◽  
Author(s):  
Yoojin Shin ◽  
Kisuk Yang ◽  
Sewoon Han ◽  
Hyun-Ji Park ◽  
Yun Seok Heo ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Three Dimensional ◽  
Neural Stem Cell Niche ◽  
Cell Niche

scholarly journals Extracellular matrix and the neural stem cell niche

2011 ◽  
Vol 71 (11) ◽  
pp. 1006-1017 ◽  
Author(s):  
Ilias Kazanis ◽  
Charles ffrench-Constant
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Neural Stem Cell Niche ◽  
Cell Niche

scholarly journals Fractone bulbs derive from ependymal cells and their laminin composition influence the stem cell niche in the subventricular zone

2016 ◽  
Author(s):  
Marcos Assis Nascimento ◽  
Lydia Sorokin ◽  
Tatiana Coelho-Sampaio
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Neural Stem Cell ◽  
Adult Neurogenesis ◽  
Subventricular Zone ◽  
Stem Cell Niche ◽  
Ependymal Cells ◽  
Cell Niche

AbstractFractones are extracellular matrix structures in the neural stem cell niche of the subventricular zone (SVZ), where they appear as round deposits named bulbs or thin branching lines called stems. Their cellular origin and what determines their localization at this site is poorly studied and it remains unclear whether they influence neural stem and progenitor cells formation, proliferation and/or maintenance. To address these questions, we analyzed whole mount preparations of the lateral ventricle by confocal microscopy using different extracellular matrix and cell markers. We found that bulbs are rarely connected to stems and that they contain laminin α5 and α2 chains, respectively. Fractone bulbs were profusely distributed throughout the SVZ and appeared associated with the center of pinwheels, a critical site for adult neurogenesis. We demonstrate that bulbs appear at the apical membrane of ependymal cells at the end of the first week after birth. The use of transgenic mice lacking laminin α5 gene expression (Lama5) in endothelium and in FoxJ1-expressing ependymal cells, revealed ependymal cells as the source of laminin α5-containing fractone bulbs. Loss of laminin α5 from bulbs correlated with a 60% increase in cell proliferation, as determined by PH3 staining, and with a selective reduction in the number of quiescent neural stem cells in the SVZ. These results indicate that fractones are a key component of the SVZ and suggest that laminin α5 modulates the physiology of the neural stem cell niche.Significance StatementOur work unveils key aspects of fractones, extracellular matrix structures present in the SVZ that still lack a comprehensive characterization. We show that fractones extensively interact with neural stem cells, whereas some of them are located precisely at pinwheel centers, which are hotspots for adult neurogenesis. Our results also demonstrate that fractones increase in size during aging and that their interactions with NSPCs become more complex in old mice. Lastly, we show that fractone bulbs are produced by ependymal cells and that their laminin content regulates neural stem cells.

scholarly journals Histological Studies of the Ventricular–Subventricular Zone as Neural Stem Cell and Glioma Stem Cell Niche

2021 ◽  
pp. 002215542110320
Author(s):  
Asa A. Brockman ◽  
Bret C. Mobley ◽  
Rebecca A. Ihrie
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tumor Cells ◽  
Neural Stem Cell ◽  
Subventricular Zone ◽  
Stem Cell Niche ◽  
Special Focus ◽  
Neural Stem Cell Niche ◽  
Stem And Progenitor Cells ◽  
Cell Niche

The neural stem cell niche of the ventricular–subventricular zone supports the persistence of stem and progenitor cells in the mature brain. This niche has many notable cytoarchitectural features that affect the activity of stem cells and may also support the survival and growth of invading tumor cells. Histochemical studies of the niche have revealed many proteins that, in combination, can help to reveal stem-like cells in the normal or cancer context, although many caveats persist in the quest to consistently identify these cells in the human brain. Here, we explore the complex relationship between the persistent proliferative capacity of the neural stem cell niche and the malignant proliferation of brain tumors, with a special focus on histochemical identification of stem cells and stem-like tumor cells and an eye toward the potential application of high-dimensional imaging approaches to the field.

Elements of a Neural Stem Cell Niche Derived from Embryonic Stem Cells

2007 ◽  
Vol 16 (6) ◽  
pp. 1017-1026 ◽  
Author(s):  
Chris Pierret ◽  
Kathleen Spears ◽  
Jason A. Morrison ◽  
Joel A. Maruniak ◽  
Martin L. Katz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Embryonic Stem ◽  
Neural Stem Cell Niche ◽  
Cell Niche

scholarly journals Hypothalamic Neurons Take Center Stage in the Neural Stem Cell Niche

2017 ◽  
Vol 21 (3) ◽  
pp. 293-294 ◽  
Author(s):  
Julia P. Andreotti ◽  
Luiza Lousado ◽  
Luiz Alexandre V. Magno ◽  
Alexander Birbrair
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Hypothalamic Neurons ◽  
Center Stage ◽  
Neural Stem Cell Niche ◽  
Cell Niche
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