A Bio-inspired Platform to Modulate Myogenic Differentiation of Human Mesenchymal Stem Cells Through Focal Adhesion Regulation

2012 ◽  
Vol 2 (3) ◽  
pp. 442-449 ◽  
Author(s):  
Haiyang Yu ◽  
Chor Yong Tay ◽  
Mintu Pal ◽  
Wen Shing Leong ◽  
Huaqiong Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Focal Adhesion ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells

scholarly journals Insights into the Role of Focal Adhesion Modulation in Myogenic Differentiation of Human Mesenchymal Stem Cells

2013 ◽  
Vol 22 (1) ◽  
pp. 136-147 ◽  
Author(s):  
Haiyang Yu ◽  
Yuan Siang Lui ◽  
Sijing Xiong ◽  
Wen Shing Leong ◽  
Feng Wen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Focal Adhesion ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells

Effect of Substrate Elasticity on In Vitro Aging of Human Mesenchymal Stem Cells

2012 ◽  
Vol 1498 ◽  
pp. 39-45
Author(s):  
Courtney E. LeBlon ◽  
Caitlin R. Fodor ◽  
Tony Zhang ◽  
Xiaohui Zhang ◽  
Sabrina S. Jedlicka
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Elastic Modulus ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Immunofluorescent Staining ◽  
Differentiation Potential ◽  
Gene And Protein Expression ◽  
The Impact

ABSTRACTHuman mesenchymal stem cells (hMSCs) were routinely cultured on tissue-culture polystyrene (TCPS) to investigate the in vitro aging and cell stiffening. hMSCs were also cultured on thermoplastic polyurethane (TPU), which is a biocompatible polymer with an elastic modulus of approximately 12.9MPa, to investigate the impact of substrate elastic modulus on cell stiffening and differentiation potential. Cells were passaged over several generations on each material. At each passage, cells were subjected to osteogenic and myogenic differentiation. Local cell elastic modulus was measured at every passage using atomic force microscopy (AFM) indentation. Gene and protein expression was examined using qRT-PCR and immunofluorescent staining, respectively, for osteogenic and myogenic markers. Results show that the success of myogenic differentiation is highly reliant on the elastic modulus of the undifferentiated cells. The success of osteogenic differentiations is most likely somewhat dependent on the cell elastic modulus, as differentiations were more successful in earlier passages, when cells were softer.

Spontaneous and specific myogenic differentiation of human mesenchymal stem cells on polyethylene glycol-linked multi-walled carbon nanotube films for skeletal muscle engineering

Nanoscale ◽  
2015 ◽  
Vol 7 (43) ◽  
pp. 18239-18249 ◽  
Author(s):  
Chunyan Zhao ◽  
Henrik Andersen ◽  
Barbaros Ozyilmaz ◽  
Sundara Ramaprabhu ◽  
Giorgia Pastorin ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Polyethylene Glycol ◽  
Carbon Nanotube ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Multi Walled Carbon Nanotube ◽  
Myogenic Induction ◽  
Nanotube Films

PEG-CNT films spontaneously direct the skeletal myogenic differentiation of hMSCs in the absence of myogenic induction factors.

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