Spontaneous and specific myogenic differentiation of human mesenchymal stem cells on polyethylene glycol-linked multi-walled carbon nanotube films for skeletal muscle engineering

Nanoscale ◽  
2015 ◽  
Vol 7 (43) ◽  
pp. 18239-18249 ◽  
Author(s):  
Chunyan Zhao ◽  
Henrik Andersen ◽  
Barbaros Ozyilmaz ◽  
Sundara Ramaprabhu ◽  
Giorgia Pastorin ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Polyethylene Glycol ◽  
Carbon Nanotube ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Multi Walled Carbon Nanotube ◽  
Myogenic Induction ◽  
Nanotube Films

PEG-CNT films spontaneously direct the skeletal myogenic differentiation of hMSCs in the absence of myogenic induction factors.

scholarly journals Skeletal muscle-derived Human Mesenchymal Stem Cells: influence of different culture conditions on proliferative and myogenic capabilities

2020 ◽  
Author(s):  
Stefano Testa ◽  
Carles Sanchez Riera ◽  
Ersilia Fornetti ◽  
Federica Riccio ◽  
Claudia Fuoco ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Myogenic Differentiation ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Human Mesenchymal Stem Cells ◽  
Culture Conditions ◽  
Set Up ◽  
Skeletal Muscle Stem Cells

AbstractSkeletal muscle tissue is characterized by restrained self-regenerative capabilities, being ineffective in relation to trauma extension both in time span (e.g. chronic diseases) and in size (e.g. large trauma). For these reasons, tissue engineering and/or cellular therapies represent a valuable solution in the cases where the physiological healing process failed. Satellite cells, the putative skeletal muscle stem cells, have been the first solution explored to remedy the insufficient self-regeneration capacity. Nevertheless, some limitation related to donor age, muscle condition, expansion hitch and myogenic potentiality maintenance have limited their use as therapeutic tool. To overcome this hindrance, different stem cells population with myogenic capabilities have been investigated to evaluate their real potentiality for therapeutic approaches, but, as of today, the perfect cell candidate has not been identified yet.In this work, we analyze the characteristics of skeletal muscle-derived human Mesenchymal Stem Cells (hMSCs), showing the maintenance/increment of myogenic activity upon differential culture conditions. In particular, we investigate the influence of a commercial enriched growth medium (Cyto-Grow), and of a medium enriched with either human-derived serum (H.S.) or Platelet-rich Plasma (PrP), in order to set up a culture protocol useful for employing this cell population in clinical therapeutic strategies. The presented results reveal the remarkable effects of H.S. in the enhancement of hMSC proliferation and myogenic differentiation.

Effect of Substrate Elasticity on In Vitro Aging of Human Mesenchymal Stem Cells

2012 ◽  
Vol 1498 ◽  
pp. 39-45
Author(s):  
Courtney E. LeBlon ◽  
Caitlin R. Fodor ◽  
Tony Zhang ◽  
Xiaohui Zhang ◽  
Sabrina S. Jedlicka
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Elastic Modulus ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Immunofluorescent Staining ◽  
Differentiation Potential ◽  
Gene And Protein Expression ◽  
The Impact

ABSTRACTHuman mesenchymal stem cells (hMSCs) were routinely cultured on tissue-culture polystyrene (TCPS) to investigate the in vitro aging and cell stiffening. hMSCs were also cultured on thermoplastic polyurethane (TPU), which is a biocompatible polymer with an elastic modulus of approximately 12.9MPa, to investigate the impact of substrate elastic modulus on cell stiffening and differentiation potential. Cells were passaged over several generations on each material. At each passage, cells were subjected to osteogenic and myogenic differentiation. Local cell elastic modulus was measured at every passage using atomic force microscopy (AFM) indentation. Gene and protein expression was examined using qRT-PCR and immunofluorescent staining, respectively, for osteogenic and myogenic markers. Results show that the success of myogenic differentiation is highly reliant on the elastic modulus of the undifferentiated cells. The success of osteogenic differentiations is most likely somewhat dependent on the cell elastic modulus, as differentiations were more successful in earlier passages, when cells were softer.

scholarly journals Skeletal Muscle-Derived Human Mesenchymal Stem Cells: Influence of Different Culture Conditions on Proliferative and Myogenic Capabilities

2020 ◽  
Vol 11 ◽  
Author(s):  
Stefano Testa ◽  
Carles Sánchez Riera ◽  
Ersilia Fornetti ◽  
Federica Riccio ◽  
Claudia Fuoco ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Culture Conditions

scholarly journals Three-dimensional carbon nanotube scaffolds for long-term maintenance and expansion of human mesenchymal stem cells

2017 ◽  
Vol 105 (7) ◽  
pp. 1927-1939 ◽  
Author(s):  
Gaurav Lalwani ◽  
Michael D'agati ◽  
Anu Gopalan ◽  
Sunny C. Patel ◽  
Yahfi Talukdar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Carbon Nanotube ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Term Maintenance
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