A Universal Platform for High‐Efficiency “Engineering” Living Cells: Integration of Cell Capture, Intracellular Delivery of Biomolecules, and Cell Harvesting Functions

2019 ◽  
Vol 30 (3) ◽  
pp. 1906362 ◽  
Author(s):  
Yangcui Qu ◽  
Yanjun Zheng ◽  
Liyin Yu ◽  
Yang Zhou ◽  
Yaran Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Intracellular Delivery ◽  
Living Cells ◽  
Cell Capture ◽  
Cell Harvesting

scholarly journals Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

RSC Advances ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Xuekang Cai ◽  
Dan Wang ◽  
Yasi Gao ◽  
Long Yi ◽  
Xing Yang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Living Cells ◽  
Highly Efficient ◽  
Aromatic Azide

A fast strain-promoted azide–alkyne cycloaddition based on tetra-fluorinated aromatic azide was developed and applied to label proteins and living cells with high efficiency.

scholarly journals A boronic acid–rich dendrimer with robust and unprecedented efficiency for cytosolic protein delivery and CRISPR-Cas9 gene editing

2019 ◽  
Vol 5 (6) ◽  
pp. eaaw8922 ◽  
Author(s):  
Chongyi Liu ◽  
Tao Wan ◽  
Hui Wang ◽  
Song Zhang ◽  
Yuan Ping ◽  
...  
Keyword(s):  
Boronic Acid ◽  
Protein Delivery ◽  
High Efficiency ◽  
Intracellular Delivery ◽  
Cytosolic Protein ◽  
Native Proteins ◽  
Cytosolic Delivery ◽  
Cas9 Protein ◽  
Cargo Proteins ◽  
Positively Charged

Cytosolic protein delivery is of central importance for the development of protein-based biotechnologies and therapeutics; however, efficient intracellular delivery of native proteins remains a challenge. Here, we reported a boronic acid–rich dendrimer with unprecedented efficiency for cytosolic delivery of native proteins. The dendrimer could bind with both negatively and positively charged proteins and efficiently delivered 13 cargo proteins into the cytosol of living cells. All the delivered proteins kept their bioactivities after cytosolic delivery. The dendrimer ensures efficient intracellular delivery of Cas9 protein into various cell lines and showed high efficiency in CRISPR-Cas9 genome editing. The rationally designed boronic acid–rich dendrimer permits the development of an efficient platform with high generality for the delivery of native proteins.

scholarly journals THE PHOTOSYNTHETIC EFFICIENCY OF PHYCOCYANIN IN CHROOCOCCUS, AND THE PROBLEM OF CAROTENOID PARTICIPATION IN PHOTOSYNTHESIS

1942 ◽  
Vol 25 (4) ◽  
pp. 579-595 ◽  
Author(s):  
Robert Emerson ◽  
Charlton M. Lewis
Keyword(s):  
Quantum Yield ◽  
Absorption Spectra ◽  
Photosynthetic Efficiency ◽  
High Efficiency ◽  
Wave Length ◽  
Visible Spectrum ◽  
Living Cells ◽  
Reasonable Doubt ◽  
Carotenoid Pigments ◽  
Relative Absorption

The absorption spectra of the principal pigment components extracted from Chroococcus cells have been measured, and their sum compared with the absorption of a suspension of living cells. The agreement was sufficiently close so that it was concluded the absorption spectra of the extracted and separated pigment components could be used to obtain estimates of the relative absorption of the various components in the living cells. The quantum yield of Chroococcus photosynthesis was measured at a succession of wave lengths throughout the visible spectrum, and the dependence of yield on wave length was compared with the proportions of light absorbed by the pigment components. This comparison showed beyond reasonable doubt that the light absorbed by phycocyanin is utilized in photosynthesis with an efficiency approximately equal to that of the light absorbed by chlorophyll. The light absorbed by the carotenoid pigments of Chroococcus seems for the most part to be unavailable for photosynthesis. The results leave open the possibility that light absorbed by the carotenoids is active in photosynthesis, but with an efficiency considerably lower than that of chlorophyll and phycocyanin. It is also possible that the light absorbed by one or a few of the several carotenoid components is utilized with a high efficiency, while the light absorbed by most of the components is lost for photosynthesis.

scholarly journals Intracellular Delivery of Membrane Impermeable Photostable Fluorescent Probes into Living Cells for Super-Resolution Microscopy

2017 ◽  
Vol 112 (3) ◽  
pp. 21a
Author(s):  
Yuji Ishitsuka ◽  
Kai Wen Teng ◽  
Pin Ren ◽  
Yeoan Youn ◽  
Xiang Deng ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Super Resolution ◽  
Intracellular Delivery ◽  
Living Cells ◽  
Super Resolution Microscopy

scholarly journals High-efficiency labeling of sialylated glycoproteins on living cells

2009 ◽  
Vol 6 (3) ◽  
pp. 207-209 ◽  
Author(s):  
Ying Zeng ◽  
T N C Ramya ◽  
Anouk Dirksen ◽  
Philip E Dawson ◽  
James C Paulson
Keyword(s):  
High Efficiency ◽  
Living Cells ◽  
Sialylated Glycoproteins

A Photothermal Nanoplatform with Sugar-Triggered Cleaning Ability for High-Efficiency Intracellular Delivery

2022 ◽  
Author(s):  
Kunyan Lu ◽  
Yangcui Qu ◽  
Yuancheng Lin ◽  
Luohuizi Li ◽  
Yan Wu ◽  
...  
Keyword(s):  
High Efficiency ◽  
Intracellular Delivery

Hydrodynamic shuttling for deterministic high-efficiency multiple single-cell capture in a microfluidic chip

Lab on a Chip ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1370-1377 ◽  
Author(s):  
Cheng-Kun He ◽  
Ya-Wen Chen ◽  
Ssu-Han Wang ◽  
Chia-Hsien Hsu
Keyword(s):  
Single Cell ◽  
Biomedical Research ◽  
Microfluidic Chip ◽  
High Efficiency ◽  
Single Cells ◽  
Cellular Heterogeneity ◽  
Cell Capture

A new microfluidics technique for high-efficiency paring and analyzing multiple single cells can facilitate cellular heterogeneity studies important for biological and biomedical research.

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