NIR‐II Excitable Conjugated Polymer Dots with Bright NIR‐I Emission for Deep In Vivo Two‐Photon Brain Imaging Through Intact Skull

2019 ◽  
Vol 29 (15) ◽  
pp. 1808365 ◽  
Author(s):  
Shaowei Wang ◽  
Jie Liu ◽  
Guangxue Feng ◽  
Lai Guan Ng ◽  
Bin Liu
Keyword(s):  
Brain Imaging ◽  
Conjugated Polymer ◽  
Two Photon ◽  
Polymer Dots

Deep-red polymer dots with bright two-photon fluorescence and high biocompatibility for in vivo mouse brain imaging

2017 ◽  
Vol 399 ◽  
pp. 120-126 ◽  
Author(s):  
Nuernisha Alifu ◽  
Zezhou Sun ◽  
Abudureheman Zebibula ◽  
Zhenggang Zhu ◽  
Xinyuan Zhao ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Mouse Brain ◽  
Two Photon ◽  
Polymer Dots ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals In vivo multiphoton fluorescence imaging with polymer dots

2017 ◽  
Author(s):  
Ahmed M. Hassan ◽  
Xu Wu ◽  
Jeremy W. Jarrett ◽  
Shihan Xu ◽  
David R. Miller ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Deep Imaging ◽  
Polymer Dots ◽  
Versatile Tool ◽  
Cortical Imaging ◽  
Semiconducting Polymer Dots

AbstractDeep in vivo imaging of vasculature requires small, bright, and photostable fluorophores suitable for multiphoton microscopy (MPM). Although semiconducting polymer dots (pdots) are an emerging class of highly fluorescent contrast agents with favorable advantages for the next generation of in vivo imaging, their use for deep multiphoton imaging has never before been demonstrated. Here we characterize the multiphoton properties of three pdot variants (CNPPV, PFBT, and PFPV) and demonstrate deep imaging of cortical microvasculature in C57 mice. Specifically, we measure the two-versus three-photon power dependence of these pdots and observe a clear three-photon excitation signature at wavelengths longer than 1300 nm, and a transition from two-photon to three-photon excitation within a 1060 – 1300 nm excitation range. Furthermore, we show that pdots enable in vivo two-photon imaging of cerebrovascular architecture in mice up to 850 μm beneath the pial surface using 800 nm excitation. In contrast with traditional multiphoton probes, we also demonstrate that the broad multiphoton absorption spectrum of pdots permits imaging at longer wavelengths (λex = 1,060 and 1225 nm). These wavelengths approach an ideal biological imaging wavelength near 1,300 nm and confer compatibility with a high-power ytterbium-fiber laser and a high pulse energy optical parametric amplifier, resulting in substantial improvements in signal-to-background ratio (>3.5-fold) and greater cortical imaging depths of 900 μm and 1300 μm. Ultimately, pdots are a versatile tool for MPM due to their extraordinary brightness and broad absorption, which will undoubtedly unlock the ability to interrogate deep structures in vivo.

scholarly journals Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes

2020 ◽  
Vol 6 (40) ◽  
pp. eabc6521 ◽  
Author(s):  
Zhongya Qin ◽  
Congping Chen ◽  
Sicong He ◽  
Ye Wang ◽  
Kam Fai Tam ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Adaptive Optics ◽  
Critical Role ◽  
Random Access ◽  
Brain Structures ◽  
Invasive Approach ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

Optical deep-brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep-brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

Near-Infrared (NIR)-Absorbing Conjugated Polymer Dots as Highly Effective Photothermal Materials for In Vivo Cancer Therapy

2016 ◽  
Vol 28 (23) ◽  
pp. 8669-8675 ◽  
Author(s):  
Shengliang Li ◽  
Xiaoyu Wang ◽  
Rong Hu ◽  
Hui Chen ◽  
Meng Li ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Conjugated Polymer ◽  
Near Infrared ◽  
Polymer Dots ◽  
Highly Effective

Simultaneous near-infrared and green fluorescence from single conjugated polymer dots with aggregation-induced emission fluorogen for cell imaging

2018 ◽  
Vol 6 (47) ◽  
pp. 7871-7876 ◽  
Author(s):  
Sitong Chen ◽  
Shuang Cui ◽  
Rongxin Du ◽  
Ming Liu ◽  
Wei-Kai Tsai ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Near Infrared ◽  
Cell Imaging ◽  
Green Fluorescence ◽  
Aggregation Induced Emission ◽  
Polymer Dots

Highly fluorescent conjugated polymer dots with aggregation-induced emission fluorogen were applied in in vitro and in vivo cell imaging.

Bright AIEgen–Protein Hybrid Nanocomposite for Deep and High‐Resolution In Vivo Two‐Photon Brain Imaging

2019 ◽  
Vol 29 (29) ◽  
pp. 1902717 ◽  
Author(s):  
Shaowei Wang ◽  
Fang Hu ◽  
Yutong Pan ◽  
Lai Guan Ng ◽  
Bin Liu
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Hybrid Nanocomposite ◽  
Two Photon

In vivo brain imaging using a portable 39?gram two-photon fluorescence microendoscope

2005 ◽  
Vol 30 (17) ◽  
pp. 2272 ◽  
Author(s):  
Benjamin A. Flusberg ◽  
Juergen C. Jung ◽  
Eric D. Cocker ◽  
Erik P. Anderson ◽  
Mark J. Schnitzer
Keyword(s):  
Brain Imaging ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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