Thermosensitive, Stretchable, and Piezoelectric Substrate for Generation of Myogenic Cell Sheet Fragments from Human Mesenchymal Stem Cells for Skeletal Muscle Regeneration

2017 ◽  
Vol 27 (48) ◽  
pp. 1703853 ◽  
Author(s):  
Jeong-Kee Yoon ◽  
Mirnmoy Misra ◽  
Seung Jung Yu ◽  
Han Young Kim ◽  
Suk Ho Bhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Muscle Regeneration ◽  
Cell Sheet ◽  
Human Mesenchymal Stem Cells ◽  
Myogenic Cell ◽  
Skeletal Muscle Regeneration ◽  
Piezoelectric Substrate

scholarly journals Effects of Human Mesenchymal Stem Cells Isolated from Wharton’s Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model

2014 ◽  
Vol 2014 ◽  
pp. 1-16 ◽  
Author(s):  
T. Pereira ◽  
P. A. S. Armada-da Silva ◽  
I. Amorim ◽  
A. Rêma ◽  
A. R. Caseiro ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Muscle Regeneration ◽  
Human Mesenchymal Stem Cells ◽  
Conditioned Media ◽  
Histological Evaluation ◽  
Type I

Skeletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. Thein vivoapplication of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expanded, associated with different vehicles to induce muscle regeneration, was evaluated in a rat myectomy model. Two commercially available vehicles and a spherical hydrogel developed by our research group were used. The treated groups obtained interesting results in terms of muscle regeneration, both in the histological and in the functional assessments. A less evident scar tissue, demonstrated by collagen type I quantification, was present in the muscles treated with HMSCs or their CM. In terms of the histological evaluation performed by ISO 10993-6 scoring, it was observed that HMSCs apparently have a long-term negative effect, since the groups treated with CM presented better scores. CM could be considered an alternative to thein vivotransplantation of these cells, as it can benefit from the local tissue response to secreted molecules with similar results in terms of muscular regeneration. Searching for an optimal vehicle might be the key point in the future of skeletal muscle tissue engineering.

scholarly journals Skeletal Muscle-Derived Human Mesenchymal Stem Cells: Influence of Different Culture Conditions on Proliferative and Myogenic Capabilities

2020 ◽  
Vol 11 ◽  
Author(s):  
Stefano Testa ◽  
Carles Sánchez Riera ◽  
Ersilia Fornetti ◽  
Federica Riccio ◽  
Claudia Fuoco ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Culture Conditions
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