The application of bone marrow mesenchymal stem cells and biomaterials in skeletal muscle regeneration

Abstract Background The skeletal muscle reconstruction occurs thanks to unipotent stem cells, i.e., satellite cells. The satellite cells remain quiescent and localized between myofiber sarcolemma and basal lamina. They are activated in response to muscle injury, proliferate, differentiate into myoblasts, and recreate myofibers. The stem and progenitor cells support skeletal muscle regeneration, which could be disturbed by extensive damage, sarcopenia, cachexia, or genetic diseases like dystrophy. Many lines of evidence showed that the level of oxygen regulates the course of cell proliferation and differentiation. Methods In the present study, we analyzed hypoxia impact on human and pig bone marrow-derived mesenchymal stromal cell (MSC) and mouse myoblast proliferation, differentiation, and fusion. Moreover, the influence of the transplantation of human bone marrow-derived MSCs cultured under hypoxic conditions on skeletal muscle regeneration was studied. Results We showed that bone marrow-derived MSCs increased VEGF expression and improved myogenesis under hypoxic conditions in vitro. Transplantation of hypoxia preconditioned bone marrow-derived MSCs into injured muscles resulted in the improved cell engraftment and formation of new vessels. Conclusions We suggested that SDF-1 and VEGF secreted by hypoxia preconditioned bone marrow-derived MSCs played an essential role in cell engraftment and angiogenesis. Importantly, hypoxia preconditioned bone marrow-derived MSCs more efficiently engrafted injured muscles; however, they did not undergo myogenic differentiation.

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Proliferation of Bone Marrow Mesenchymal Stem Cells, Skeletal Muscle Cells and Co-Culture of Both for Cell Myocardium Therapy in Wistar Rats

Transplantation Proceedings ◽

10.1016/j.transproceed.2006.06.068 ◽

2006 ◽

Vol 38 (6) ◽

pp. 1955-1956 ◽

Cited By ~ 1

Author(s):

K.A.T. Carvalho ◽

L.C. Guarita-Souza ◽

R.B. Simeone ◽

J.C. Francisco ◽

M. Olandoski ◽

...

Keyword(s):

Skeletal Muscle ◽

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Wistar Rats ◽

Muscle Cells ◽

Skeletal Muscle Cells ◽

Marrow Mesenchymal Stem Cells

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Hypoxic Preconditioned Bone Marrow-Derived Mesenchymal Stromal/Stem Cells Enhance Myoblast Fusion and Skeletal Muscle Regeneration

10.21203/rs.3.rs-474083/v1 ◽

2021 ◽

Author(s):

Karolina Archacka ◽

Iwona Grabowska ◽

Bartosz Mierzejewski ◽

Joanna Graffstain ◽

Alicja Górzyńska ◽

...

Keyword(s):

Skeletal Muscle ◽

Stem Cells ◽

Bone Marrow ◽

Satellite Cells ◽

Muscle Regeneration ◽

Myogenic Differentiation ◽

Skeletal Muscle Regeneration ◽

Hypoxic Conditions ◽

Cell Engraftment

Abstract Background: The skeletal muscle reconstruction occurs thanks to unipotent stem cells, i.e., satellite cells. The satellite cells remain quiescent and localized between myofiber sarcolemma and basal lamina. They are activated in response to muscle injury, proliferate, differentiate into myoblasts, and recreate myofibers. Many stem and progenitor cells support skeletal muscle regeneration, which could be disturbed by extensive damage, sarcopenia, cachexia, or genetic diseases like dystrophy. Many lines of evidence showed that the level of oxygen regulates the course of cell proliferation and differentiation. Methods: In the present study, we analyzed hypoxic’s impact on human and pig bone marrow-derived mesenchymal stromal cell (MSC) and mouse myoblast proliferation, differentiation, and fusion. Moreover, the influence of the transplantation of human bone marrow-derived MSCs cultured under hypoxic conditions on skeletal muscle regeneration was studied. Results: We showed that bone marrow-derived MSCs increased VEGF expression and improved myogenesis under hypoxic conditions in vitro. Transplantation of hypoxic preconditioned bone marrow-derived MSCs into injured muscles resulted in the improved cell engraftment and formation of new vessels. Conclusions: We suggested that SDF-1 and VEGF secreted by hypoxic preconditioned bone marrow-derived MSCs played an essential role in cell engraftment and angiogenesis. Importantly, hypoxic preconditioned bone marrow-derived MSCs more efficiently engrafted injured muscles, however, they did not undergo myogenic differentiation.

Download Full-text