Tissue Engineering: Stable Extracellular Matrix Protein Patterns Guide the Orientation of Osteoblast-like Cells (Adv. Funct. Mater. 21/2011)

2011 ◽  
Vol 21 (21) ◽  
pp. 4199-4199
Author(s):  
Jian-Tao Zhang ◽  
Juequan Nie ◽  
Mike Mühlstädt ◽  
Hilary Gallagher ◽  
Oliver Pullig ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Protein Patterns

Stable Extracellular Matrix Protein Patterns Guide the Orientation of Osteoblast-like Cells

2011 ◽  
Vol 21 (21) ◽  
pp. 4079-4087 ◽  
Author(s):  
Jian-Tao Zhang ◽  
Juequan Nie ◽  
Mike Mühlstädt ◽  
Hilary Gallagher ◽  
Oliver Pullig ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Protein Patterns

Stem cell differentiation: Controlling Differentiation of Neural Stem Cells Using Extracellular Matrix Protein Patterns (Small 22/2010)

Small ◽  
2010 ◽  
Vol 6 (22) ◽  
pp. 2508-2508
Author(s):  
Aniruddh Solanki ◽  
Shreyas Shah ◽  
Kevin A. Memoli ◽  
Sung Young Park ◽  
Seunghun Hong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Neural Stem Cells ◽  
Matrix Protein ◽  
Stem Cell Differentiation ◽  
Extracellular Matrix Protein ◽  
Protein Patterns

scholarly journals Controlling Differentiation of Neural Stem Cells Using Extracellular Matrix Protein Patterns

Small ◽  
2010 ◽  
Vol 6 (22) ◽  
pp. 2509-2513 ◽  
Author(s):  
Aniruddh Solanki ◽  
Shreyas Shah ◽  
Kevin A. Memoli ◽  
Sung Young Park ◽  
Seunghun Hong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Neural Stem Cells ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Protein Patterns

1280: Increased Susceptibility to Genitovaginal Prolapse Associated with a Polymorphism in the Promoter of the Extracellular Matrix Protein LAMC1

2007 ◽  
Vol 177 (4S) ◽  
pp. 421-422
Author(s):  
Ganka Nikolova ◽  
Christian O. Twiss ◽  
Hane Lee ◽  
Nelson Stanley ◽  
Janet Sinsheimer ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Increased Susceptibility

scholarly journals Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor

2021 ◽  
Author(s):  
Aniel Moya-Torres ◽  
Monika Gupta ◽  
Fabian Heide ◽  
Natalie Krahn ◽  
Scott Legare ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hollow Fiber ◽  
Mammalian Cells ◽  
Matrix Protein ◽  
Continuous Production ◽  
Extracellular Matrix Protein ◽  
Close Monitoring ◽  
High Quality ◽  
Biolayer Interferometry ◽  
Hollow Fiber Bioreactor

Abstract The production of recombinant proteins for functional and biophysical studies, especially in the field of structural determination, still represents a challenge as high quality and quantities are needed to adequately perform experiments. This is in part solved by optimizing protein constructs and expression conditions to maximize the yields in regular flask expression systems. Still, work flow and effort can be substantial with no guarantee to obtain improvements. This study presents a combination of workflows that can be used to dramatically increase protein production and improve processing results, specifically for the extracellular matrix protein Netrin-1. This proteoglycan is an axon guidance cue which interacts with various receptors to initiate downstream signaling cascades affecting cell differentiation, proliferation, metabolism, and survival. We were able to produce large glycoprotein quantities in mammalian cells, which were engineered for protein overexpression and secretion into the media using the controlled environment provided by a hollow fiber bioreactor. Close monitoring of the internal bioreactor conditions allowed for stable production over an extended period of time. In addition to this, Netrin-1 concentrations were monitored in expression media through biolayer interferometry which allowed us to increase Netrin-1 media concentrations tenfold over our current flask systems while preserving excellent protein quality and in solution behavior. Our particular combination of genetic engineering, cell culture system, protein purification, and biophysical characterization permitted us to establish an efficient and continuous production of high-quality protein suitable for structural biology studies that can be translated to various biological systems. Key points • Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 • Biolayer interferometry allows target protein quantitation in expression media • High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality Graphical abstract

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