High-Resolution Patterning of Hydrogels in Three Dimensions using Direct-Write Photofabrication for Cell Guidance

2009 ◽  
Vol 19 (22) ◽  
pp. 3543-3551 ◽  
Author(s):  
Stephanie K. Seidlits ◽  
Christine E. Schmidt ◽  
Jason B. Shear
Keyword(s):  
High Resolution ◽  
Three Dimensions ◽  
Direct Write ◽  
Cell Guidance

Confocal fluorescence microscopy with the tandem scanning light microscope

1989 ◽  
Vol 94 (4) ◽  
pp. 617-624
Author(s):  
S.J. Wright ◽  
J.S. Walker ◽  
H. Schatten ◽  
C. Simerly ◽  
J.J. McCarthy ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Light Microscope ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Complex Structures ◽  
Charge Coupled Device ◽  
Confocal Fluorescence ◽  
Cooled Ccd ◽  
Cellular Components

Applications of the tandem scanning confocal microscope (TSM) to fluorescence microscopy and its ability to resolve fluorescent biological structures are described. The TSM, in conjunction with a cooled charge-coupled device (cooled CCD) and conventional epifluorescence light source and filter sets, provided high-resolution, confocal data, so that different fluorescent cellular components were distinguished in three dimensions within the same cell. One of the unique features of the TSM is the ability to image fluorochromes excited by ultraviolet light (e.g. Hoechst, DAPI) in addition to fluorescein and rhodamine. Since the illumination is dim, photobleaching is insignificant and prolonged viewing of living specimens is possible. Series of optical sections taken in the Z-axis with the TSM were reproduced as stereo images and three-dimensional reconstructions. These data show that the TSM is potentially a powerful tool in fluorescence microscopy for determining three-dimensional relationships of complex structures within cells labeled with multiple fluorochromes.

Aerosol Printing of High Resolution Films for LTCC-Multilayer Components

2012 ◽  
Vol 9 (3) ◽  
pp. 133-137 ◽  
Author(s):  
Martin Ihle ◽  
Uwe Partsch ◽  
Sindy Mosch ◽  
Adrian Goldberg
Keyword(s):  
High Resolution ◽  
High Frequency ◽  
High Reliability ◽  
Direct Write ◽  
Printing Technology ◽  
Fine Line ◽  
Aerosol Printing ◽  
Fine Structures ◽  
Cost Efficient ◽  
Printing Parameters

For the electronic packaging of sensor stable and cost-efficient fine line printing technologies on LTCC and high frequency laminates are needed. Especially common technologies like screen printing and thin film techniques are unsuitable for fine structures or too expensive. In addition, there is no direct write technology for 3D LTCC designs as well as for high reliability cofiring structures. Closing this gap, aerosol printing technology is used to print high resolution conductors on planar and nonplanar substrates. Aerosol printing is a direct write noncontact printing technology of functional layers. After pneumatic atomization, the ink is transformed into 1–5 μm droplets. The resulting continuous aerosol stream is focused by a sheath gas in the printing head. Thus, the long standoff distance between the substrate and the deposition tip of max. 5 mm allows 3D printing on nonplanar substrates. With optimized inks and printing parameters, line widths of 10 μm are achievable. This paper will present applications for aerosol printed functional layers on LTCC. These are, for example, aerosol printed films embedded in cofired LTCC, fine line structures for high frequency applications, and the evaluation of printed 3D structures like LTCC stairways. Furthermore, the 90° contact of unconventional sensor designs will be presented.

SEM Stop-Frame, Color, 3D Animation for Motion Pictures

1998 ◽  
Vol 4 (S2) ◽  
pp. 16-17
Author(s):  
David Scharf ◽  
Jacob Wilbrink ◽  
John A. Hunt
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Three Dimensions ◽  
3D Animation ◽  
Special Effects ◽  
Projection Screen ◽  
Traditional Format ◽  
Stereo Motion ◽  
Large Projection ◽  
Animation System

A stop-frame animation system has been developed for producing high-resolution, color and stereo motion picture animation sequences. The first of these sequences can be seen in the recently released IMAX 3D movies, “Four Million House Guests” a.k.a. “The Hidden Dimension”. IMAX movies have long been known for their breathtaking special effects that seem incredibly realistic because of the large projection screen (about 7 stories high) which is close to the entire audience, high resolution, and powerful audio effects. IMAX 3D is an extention to the traditional format that allows the audience to see three dimensional special effects with the aid of electronically shuttered viewing glasses. IMAX movies are an ideal medium to demonstrate the high resolution digital images that are possible with the SEM.The goal of the SEM movie project was to produce movie sequences where viewers feels like they are flying smoothly through micro-space past microscopic creatures and objects in three dimensions and in color.

High-resolution direct-write multiphoton photolithography in poly(methylmethacrylate) films

2006 ◽  
Vol 88 (18) ◽  
pp. 184101 ◽  
Author(s):  
Daniel A. Higgins ◽  
Thomas A. Everett ◽  
Aifang Xie ◽  
Sarah M. Forman ◽  
Takashi Ito
Keyword(s):  
High Resolution ◽  
Direct Write

scholarly journals Bathymetry of Northwest Greenland Using “Ocean Melting Greenland” (OMG) High-Resolution Airborne Gravity and Other Data

2019 ◽  
Vol 11 (2) ◽  
pp. 131 ◽  
Author(s):  
Lu An ◽  
Eric Rignot ◽  
Romain Millan ◽  
Kirsty Tinto ◽  
Josh Willis
Keyword(s):  
High Resolution ◽  
Continental Shelf ◽  
Gravity Data ◽  
Three Dimensional ◽  
Ice Sheet ◽  
Greenland Ice Sheet ◽  
Three Dimensions ◽  
Airborne Gravity ◽  
Lack Of Information ◽  
Sea Bed

Marine-terminating glaciers dominate the evolution of the Greenland Ice Sheet (GrIS) and its contribution to sea-level rise. Widespread glacier acceleration has been linked to the warming of ocean waters around the periphery of Greenland but a lack of information on the bathymetry of the continental shelf and glacial fjords has limited our ability to understand how subsurface, warm, salty ocean waters of Atlantic origin (AW) reach the glaciers and melt them from below. Here, we employ high-resolution, airborne gravity data (AIRGrav) in combination with multibeam echo sounding (MBES) data, to infer the bathymetry of the coastal areas of Northwest Greenland for NASA’s Ocean Melting Greenland (OMG) mission. High-resolution, AIRGrav data acquired on a 2 km spacing, 150 m ground clearance, with 1.5 mGal crossover error, is inverted in three dimensions to map the bathymetry. To constrain the inversion away from MBES data, we compare two methods: one based on the Direct Current (DC) shift of the gravity field (absolute minus observed gravity) and another based on the density of the bedrock. We evaluate and compare the two methods in areas with complete MBES coverage. We find the lowest standard error in bed elevation (±60 m) using the DC shift method. When applied to the entire coast of Northwest Greenland, the three-dimensional inversion reveals a complex network of connected sea bed channels, not known previously, that provide natural and varied pathways for AW to reach the glaciers across the continental shelf. The study demonstrates that the gravity approach offers an efficient and practical alternative to extensive ship mapping in ice-filled waters to obtain information critical to understanding and modeling ice-ocean interaction along ice sheet margins.

scholarly journals Reconstruction of Axial Tomographic High Resolution Data from Confocal Fluorescence Microscopy: A Method for Improving 3D FISH Images

2000 ◽  
Vol 20 (1) ◽  
pp. 7-15 ◽  
Author(s):  
R. Heintzmann ◽  
G. Kreth ◽  
C. Cremer
Keyword(s):  
High Resolution ◽  
Laser Scanning ◽  
Axial Direction ◽  
Laser Scanning Microscopy ◽  
Three Dimensions ◽  
Confocal Laser ◽  
Data Sets ◽  
Data Set ◽  
High Resolution Data ◽  
3D Data

Fluorescent confocal laser scanning microscopy allows an improved imaging of microscopic objects in three dimensions. However, the resolution along the axial direction is three times worse than the resolution in lateral directions. A method to overcome this axial limitation is tilting the object under the microscope, in a way that the direction of the optical axis points into different directions relative to the sample. A new technique for a simultaneous reconstruction from a number of such axial tomographic confocal data sets was developed and used for high resolution reconstruction of 3D‐data both from experimental and virtual microscopic data sets. The reconstructed images have a highly improved 3D resolution, which is comparable to the lateral resolution of a single deconvolved data set. Axial tomographic imaging in combination with simultaneous data reconstruction also opens the possibility for a more precise quantification of 3D data. The color images of this publication can be accessed from http://www.esacp.org/acp/2000/20‐1/heintzmann.htm. At this web address an interactive 3D viewer is additionally provided for browsing the 3D data. This java applet displays three orthogonal slices of the data set which are dynamically updated by user mouse clicks or keystrokes.

scholarly journals Surface imaging microscopy, an automated method for visualizing whole embryo samples in three dimensions at high resolution

2002 ◽  
Vol 225 (3) ◽  
pp. 369-375 ◽  
Author(s):  
Andrew J. Ewald ◽  
Helen Mcbride ◽  
Mark Reddington ◽  
Scott E. Fraser ◽  
Russell Kerschmann
Keyword(s):  
High Resolution ◽  
Three Dimensions ◽  
Surface Imaging ◽  
Automated Method

High Resolution 3-D Laser Direct-Write Patterning

2010 ◽  
Author(s):  
Linjie Li ◽  
Rafael R. Gattass ◽  
Michael Stocker ◽  
Erez Gershgoren ◽  
Hana Hwang ◽  
...  
Keyword(s):  
High Resolution ◽  
Direct Write ◽  
Laser Direct Write
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