Optimization of gluconic acid synthesis by removing limitations and inhibitions

1993 ◽  
Vol 13 (3) ◽  
pp. 257-268 ◽  
Author(s):  
H.-D. Pöhland ◽  
V. Schierz ◽  
R. Schumann
Keyword(s):  
Gluconic Acid ◽  
Acid Synthesis

scholarly journals Multi-Omics Reveal the Efficient Phosphate-Solubilizing Mechanism of Bacteria on Rocky Soil

2021 ◽  
Vol 12 ◽  
Author(s):  
Yanqiang Ding ◽  
Zhuolin Yi ◽  
Yang Fang ◽  
Sulan He ◽  
Yuming Li ◽  
...  
Keyword(s):  
Gluconic Acid ◽  
Agricultural Development ◽  
Tricarboxylic Acid Cycle ◽  
Acid Synthesis ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Fertilizers ◽  
Plant Growth Promoting ◽  
Rocky Soil ◽  
Phosphate Solubilizing

Phosphate-solubilizing bacteria (PSB) can alleviate available phosphorus (AP)-deficiency without causing environmental pollution like chemical phosphate fertilizers. However, the research and application of PSB on the barren rocky soil is very rare. We screened six PSB from sweetpotato rhizosphere rocky soil. Among them, Ochrobactrum haematophilum FP12 showed the highest P-solubilizing ability of 1,085.00 mg/L at 7 days, which was higher than that of the most reported PSB. The assembled genome of PSB FP12 was 4.92 Mb with P-solubilizing and plant growth-promoting genes. In an AP-deficient environment, according to transcriptome and metabolomics analysis, PSB FP12 upregulated genes involved in gluconic acid synthesis and the tricarboxylic acid cycle, and increased the concentration of gluconic acid and malic acid, which would result in the enhanced P-solubilizing ability. Moreover, a series of experiments in the laboratory and field confirmed the efficient role of the screened PSB on significantly increasing AP in the barren rocky soil and promoting sweetpotato yield. So, in this study, we screened highly efficient PSB, especially suitable for the barren rocky soil, and explored the P-solubilizing mechanism. The research will reduce the demand for chemical phosphate fertilizers and promote the environment-friendly agricultural development.

scholarly journals Improvement ofAspergillus nigerfor Sodium Gluconate Synthesis by UV Mutation Method

2012 ◽  
Vol 9 (4) ◽  
pp. 2052-2057 ◽  
Author(s):  
Rajagopalan Prabu ◽  
Thomas Chand ◽  
Sunhare Raksha
Keyword(s):  
Gluconic Acid ◽  
Parent Strain ◽  
Batch Fermentation ◽  
Glucose Dehydrogenase ◽  
Acid Synthesis ◽  
Sodium Gluconate ◽  
Random Mutation ◽  
Simple Method ◽  
Uv Mutation ◽  
Acid Pathway

The sodium gluconate synthesis pathway was improved inAspergillus nigerby random mutation method.A. nigerwas mutated with Ultraviolet (UV) radiation and the alteration of cell bound enzymes activity of gluconic acid synthesis pathway and sodium gluconate synthesis were evaluated. The improved mutants (A. nigerUV-112) was capable of producing sodium gluconate up to final concentrations of 60 g/L in batch fermentation, which was 3.0 fold higher than the parent strain. It have been observed that the changes in activities of cell bound enzymes related for gluconic acid pathway such as glucose dehydrogenase and glucose oxidase and it were significantly higher than the parent strain. The mutantA. nigerstrain and the simple method used to decrease the production cost and development of fermentation process for industrial production of gluconic acid or its salt.

Gluconic acid synthesis by the ectomycorrhizal fungus Tricholoma robustum

1992 ◽  
Vol 70 (1) ◽  
pp. 84-88 ◽  
Author(s):  
Koji Iwase
Keyword(s):  
Glucose Oxidase ◽  
Gluconic Acid ◽  
High Performance ◽  
Culture Medium ◽  
Acid Synthesis ◽  
Ectomycorrhizal Fungus ◽  
Tricholoma Matsutake ◽  
Mycelial Culture ◽  
High Productivity ◽  
Tricholoma Bakamatsutake

During mycelial culture of Tricholoma robustum, the medium gradually became acidified to approximately pH 3.9. High performance liquid chromatography showed that gluconic acid was secreted into the culture medium, and the amount of gluconic acid produced was measured by enzymatic analysis. Gluconic acid synthesis by all other related species, Tricholoma matsutake, Tricholoma caligatum, Tricholoma ponderosum, Tricholoma fulvocastaneum, and Tricholoma zelleri was poor, except for Tricholoma bakamatsutake, which showed relatively high productivity. Activity of glucose oxidase, which is responsible for gluconic acid production, was highest in T. robustum and second highest in T. bakamatsutake. The activity in these two species was much higher than those of other species. These results indicate that gluconic acid was synthesized from glucose by glucose oxidase in T. robustum as well as in T. bakamatsutake. Key words: ectomycorrhizal fungi, gluconic acid, glucose oxidase, Tricholoma robustum.

Genipin Cross-Linked Glucose Oxidase and Catalase Multi-enzyme for Gluconic Acid Synthesis

2016 ◽  
Vol 181 (2) ◽  
pp. 526-535 ◽  
Author(s):  
Caixia Cui ◽  
Haibin Chen ◽  
Biqiang Chen ◽  
Tianwei Tan
Keyword(s):  
Glucose Oxidase ◽  
Gluconic Acid ◽  
Acid Synthesis

Carbohydrate metabolism and gluconic acid synthesis by Botrytis cinerea

1989 ◽  
Vol 67 (10) ◽  
pp. 2888-2893 ◽  
Author(s):  
Bernard Donèche
Keyword(s):  
Glucose Oxidase ◽  
Gluconic Acid ◽  
Tricarboxylic Acid Cycle ◽  
Glyoxylate Cycle ◽  
Acid Synthesis ◽  
Direct Oxidation ◽  
Plant Parasite ◽  
Acid Accumulation ◽  
Oxidation Of Glucose ◽  
The Glyoxylate Cycle

The pathways of glucose catabolism were examined in a B. cinerea strain isolated from grape. Respirometric and enzymatic studies indicated that this plant parasite catabolized glucose through the Embden–Meyerhof and hexose monophosphate shunt pathways. Data also suggested functioning of an active tricarboxylic acid cycle and presence of the glyoxylate cycle. Direct oxidation of glucose by means of glucose oxidase led to gluconic acid accumulation in the medium during the stationary phase of growth. Part of the glucose oxidase was extracellular and could have technological consequences in wine making.

scholarly journals Gluconic Acid Synthesis in an Electroenzymatic Reactor

2015 ◽  
Vol 174 ◽  
pp. 480-487 ◽  
Author(s):  
M. Varničić ◽  
T. Vidaković-Koch ◽  
K. Sundmacher
Keyword(s):  
Gluconic Acid ◽  
Acid Synthesis
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