A Syringe Injection into Hot Vaporizing Chambers

Keyword(s):  
1972 ◽  
Vol 51 (2) ◽  
pp. 573-576 ◽  
Author(s):  
Norman K. Ikehara ◽  
David H. Mc Kibben ◽  
Jack L. Pechersky ◽  
Robert Rapp

A biopsy desiccation method was used to demonstrate the production of edema fluid after administration of a local anesthetic solution by jet and needle-syringe injection techniques. The jet injection technique produced less edema fluid than the needle-syringe injection technique.


Author(s):  
A.A. Ayu Asri Prima Dewi ◽  
Radiana D. Antarianto ◽  
Jeanne Adiwinata Pawitan

Liver biological scaffold was developed in order to resemble native liver tissue environment. It can be achieved by decellularizing native liver tissue that will remove cells and preserve extracellular matrix (ECM). Furthermore, ECM fibers are arranged in a special pattern, which affect liver cell polarity and topography that are important for cells’ implantation, proliferation and differentiation. Therefore, the aim of this study was to evaluate liver cube scaffold topography that was decellularized with fixed multiple sites syringe injection (Indonesia patent number: S00201907930).Rat liver cubes (n=3) underwent decellularization with Ethylene Glycol Tetraacetic Acid (EGTA) immersion and increased Sodium Dodecyl Sulfate (SDS) concentrations using previous multiple sites syringe injection protocol study. Deoxyribonucleic Acid (DNA) concentrations were measured to confirm less DNA materials remaining in scaffolds. Scanning Electron Microscope (SEM) analysis of scaffolds were conducted for topographic characterization compared to undecellularized liver control. Molecular analysis of DNA concentration showed complete removal of DNA material. SEM analysis gave appearance of intact liver cube scaffold microarchitecture. Liver cubes decellularization using multiple sites syringe injection showed good topographic liver scaffold characterization.


Plant Disease ◽  
2011 ◽  
Vol 95 (5) ◽  
pp. 523-529 ◽  
Author(s):  
Louis K. Prom ◽  
Ramasamy Perumal ◽  
Saradha R. Erattaimuthu ◽  
John E. Erpelding ◽  
Noe Montes ◽  
...  

Head smut, caused by the fungal pathogen Sporisorium reilianum, has been reported with increasing frequency in the grain sorghum growing areas of Texas. To facilitate analysis of changes in pathogen virulence, four inoculation techniques were examined: soil and teliospore mixture, seed coating, media placement, and syringe injection. Of the four, syringe injection was determined to be the most effective. Inoculations of sorghum host differentials BTx643, BTx7078, BTx635, SC170-6-17 (TAM2571), SA281 (Early Hegari), and Tx414 showed 23 of 32 Texas isolates were race 4. Two isolates from College Station, TX, were classified as race 1, but no race 2 or 3 isolates were found. New, virulent races 5 and 6 were identified among isolates from south Texas. Using 16 amplified fragment length polymorphism (AFLP) primer combinations, genetic diversity was assessed in DNA samples from 49 S. reilianum isolates, including 44 sorghum isolates from Texas, two from Uganda, and one from Mali; and two maize isolates from Mexico. Single-base extensions with EcoRI and MseI primers in the selective amplification increased the number of informative polymorphic bands. High genetic dissimilarity (50%) was observed between isolates originating from maize and those originating from sorghum. The resultant dendrogram, made using cluster analysis, grouped the Texas S. reilianum isolates into four small clusters with ≥82% similarity. Other than for two race 6 isolates from Weslaco, TX, no evidence for geographical or other restrictions on gene flow was evident.


2000 ◽  
Vol 19 (9) ◽  
pp. 839-842 ◽  
Author(s):  
MARK J. SARNO ◽  
ERICH BLASE ◽  
NELLY GALINDO ◽  
ROBERTO RAMIREZ ◽  
CARL L. SCHIRMER ◽  
...  

Author(s):  
Thirupathi Dongala ◽  
Ashok Kumar Palakurthi ◽  
Yashodharani Vytla ◽  
Naresh Kumar Katari
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