Biotechnological Processing Steps for Enzyme Manufacture

Biocatalysis ◽  
2005 ◽  
pp. 209-242
Keyword(s):  
1997 ◽  
Vol 503 ◽  
Author(s):  
Yongxia Zhang ◽  
Yanwei Zhang ◽  
Juliana Blaser ◽  
T. S. Sriiram ◽  
R. B. Marcus

ABSTRACTA thermal microprobe has been designed and built for high resolution temperature sensing. The thermal sensor is a thin-film thermocouple junction at the tip of an Atomic Force Microprobe (AFM) silicon probe needle. Only wafer-stage processing steps are used for the fabrication. The thermal response over the range 25–s 4.5–rovolts per degree C and is linear.


2020 ◽  
pp. 45-54
Author(s):  
V.A. Zharkov

A theory and a mathematical model are developed for calculating the parameters of the strain-stressed state at the stages of pre-bending, calibration, straightening and bending of the straightened section, taking into account the strengthening of the billet on a roll machine. By CAD/CAE modeling the strains, stresses and forces acting on the billet from the rolls are investigated. Criteria for the formation of cracks, low accuracy and excessive springing of the billet after these processing steps are determined, recommendations are given for their elimination. Keywords billet, sheet stamping, bending, roll machine, pre-bending, calibration, straightening, bending of the straightened section, CAD/CAE modeling. [email protected]


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Kerstin Jedvert ◽  
Linnea Viklund ◽  
Mårten Alkhagen ◽  
Tobias Köhnke ◽  
Hans Theliander

Abstract Nonwovens are increasing in demand due to their versatility which enables use in a broad range of applications. Most nonwovens are still produced from fossil-based resources and there is thus a need to develop competitive materials from renewable feedstock. In this work, nonwovens are produced from cellulose via a direct solution blowing method. Cellulose was dissolved using the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) and was regenerated into nonwovens by coagulation in water. The properties of such nonwovens were previously rather stiff and papery-like and the aim of this work was to improve the softness and feel of the materials by simple adjustments of the post-processing steps, i. e. washing and drying. It was shown that by primarily changing the drying method, it was possible to create a much softer and bulkier material using the same solution blowing parameters.


2021 ◽  
Author(s):  
Maxwell Adam Levinson ◽  
Justin Niestroy ◽  
Sadnan Al Manir ◽  
Karen Fairchild ◽  
Douglas E. Lake ◽  
...  

AbstractResults of computational analyses require transparent disclosure of their supporting resources, while the analyses themselves often can be very large scale and involve multiple processing steps separated in time. Evidence for the correctness of any analysis should include not only a textual description, but also a formal record of the computations which produced the result, including accessible data and software with runtime parameters, environment, and personnel involved. This article describes FAIRSCAPE, a reusable computational framework, enabling simplified access to modern scalable cloud-based components. FAIRSCAPE fully implements the FAIR data principles and extends them to provide fully FAIR Evidence, including machine-interpretable provenance of datasets, software and computations, as metadata for all computed results. The FAIRSCAPE microservices framework creates a complete Evidence Graph for every computational result, including persistent identifiers with metadata, resolvable to the software, computations, and datasets used in the computation; and stores a URI to the root of the graph in the result’s metadata. An ontology for Evidence Graphs, EVI (https://w3id.org/EVI), supports inferential reasoning over the evidence. FAIRSCAPE can run nested or disjoint workflows and preserves provenance across them. It can run Apache Spark jobs, scripts, workflows, or user-supplied containers. All objects are assigned persistent IDs, including software. All results are annotated with FAIR metadata using the evidence graph model for access, validation, reproducibility, and re-use of archived data and software.


2021 ◽  
Vol 13 (15) ◽  
pp. 3021
Author(s):  
Bufan Zhao ◽  
Xianghong Hua ◽  
Kegen Yu ◽  
Xiaoxing He ◽  
Weixing Xue ◽  
...  

Urban object segmentation and classification tasks are critical data processing steps in scene understanding, intelligent vehicles and 3D high-precision maps. Semantic segmentation of 3D point clouds is the foundational step in object recognition. To identify the intersecting objects and improve the accuracy of classification, this paper proposes a segment-based classification method for 3D point clouds. This method firstly divides points into multi-scale supervoxels and groups them by proposed inverse node graph (IN-Graph) construction, which does not need to define prior information about the node, it divides supervoxels by judging the connection state of edges between them. This method reaches minimum global energy by graph cutting, obtains the structural segments as completely as possible, and retains boundaries at the same time. Then, the random forest classifier is utilized for supervised classification. To deal with the mislabeling of scattered fragments, higher-order CRF with small-label cluster optimization is proposed to refine the classification results. Experiments were carried out on mobile laser scan (MLS) point dataset and terrestrial laser scan (TLS) points dataset, and the results show that overall accuracies of 97.57% and 96.39% were obtained in the two datasets. The boundaries of objects were retained well, and the method achieved a good result in the classification of cars and motorcycles. More experimental analyses have verified the advantages of the proposed method and proved the practicability and versatility of the method.


2021 ◽  
pp. 016555152199863
Author(s):  
Ismael Vázquez ◽  
María Novo-Lourés ◽  
Reyes Pavón ◽  
Rosalía Laza ◽  
José Ramón Méndez ◽  
...  

Current research has evolved in such a way scientists must not only adequately describe the algorithms they introduce and the results of their application, but also ensure the possibility of reproducing the results and comparing them with those obtained through other approximations. In this context, public data sets (sometimes shared through repositories) are one of the most important elements for the development of experimental protocols and test benches. This study has analysed a significant number of CS/ML ( Computer Science/ Machine Learning) research data repositories and data sets and detected some limitations that hamper their utility. Particularly, we identify and discuss the following demanding functionalities for repositories: (1) building customised data sets for specific research tasks, (2) facilitating the comparison of different techniques using dissimilar pre-processing methods, (3) ensuring the availability of software applications to reproduce the pre-processing steps without using the repository functionalities and (4) providing protection mechanisms for licencing issues and user rights. To show the introduced functionality, we created STRep (Spam Text Repository) web application which implements our recommendations adapted to the field of spam text repositories. In addition, we launched an instance of STRep in the URL https://rdata.4spam.group to facilitate understanding of this study.


Metals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 182
Author(s):  
Suvi Santa-aho ◽  
Mika Kiviluoma ◽  
Tuomas Jokiaho ◽  
Tejas Gundgire ◽  
Mari Honkanen ◽  
...  

Additive manufacturing (AM) is a relatively new manufacturing method that can produce complex geometries and optimized shapes with less process steps. In addition to distinct microstructural features, residual stresses and their formation are also inherent to AM components. AM components require several post-processing steps before they are ready for use. To change the traditional manufacturing method to AM, comprehensive characterization is needed to verify the suitability of AM components. On very demanding corrosion atmospheres, the question is does AM lower or eliminate the risk of stress corrosion cracking (SCC) compared to welded 316L components? This work concentrates on post-processing and its influence on the microstructure and surface and subsurface residual stresses. The shot peening (SP) post-processing levelled out the residual stress differences, producing compressive residual stresses of more than −400 MPa in the AM samples and the effect exceeded an over 100 µm layer below the surface. Post-processing caused grain refinement and low-angle boundary formation on the sample surface layer and silicon carbide (SiC) residue adhesion, which should be taken into account when using the components. Immersion tests with four-point-bending in the heated 80 °C magnesium chloride solution for SCC showed no difference between AM and reference samples even after a 674 h immersion.


2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Timo Homburg ◽  
Anja Cramer ◽  
Laura Raddatz ◽  
Hubert Mara

AbstractMotivated by the increased use of 3D acquisition of objects by cultural heritage institutions, we were investigating ontologies and metadata schemes for the acquisition process to provide details about the 3D capturing, which can be combined with preexisting ontologies describing an object. Therefore we divided the 3D capturing workflow into common steps starting with the object being placed in front of a 3D scanner to preparation and publication of the 3D datasets and/or derived images. While the proposed ontology is well defined on a coarse level of detail for very different techniques, e.g. Stucture from Motion and LiDAR we elaborated the metadata scheme in very fine detail for 3D scanners available at our institutions. This includes practical experiments with measurement data from past and current projects including datasets published at Zenodo as guiding examples and the source code for their computation. Additionally, the free and Open Source GigaMesh Software Framework’s analysis and processing methods have been extended to provide metadata about the 3D processing steps like mesh cleaning as well as 2D image generation. Finally, we discuss the current limitations and give an outlook about future extensions.


Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1458 ◽  
Author(s):  
Mariana Lopes ◽  
Chloé Pierrepont ◽  
Carla Margarida Duarte ◽  
Alexandra Filipe ◽  
Bruno Medronho ◽  
...  

Recently, milk consumption has been declining and there is a high demand for non-dairy beverages. However, market offers are mainly cereal and nut-based beverages, which are essentially poor in protein (typically, less than 1.5% against the 3.5% in milk) and are not true milk replacers in that sense. In this work, new beverages from different pulses (i.e., pea, chickpea and lupin) were developed using technologies that enable the incorporation of a high level of seed components, with low or no discharge of by-products. Different processing steps were sequentially tested and discussed for the optimization of the sensorial features and stability of the beverage, considering the current commercial non-dairy beverages trends. The lupin beverage protein contents ranged from 1.8% to 2.4% (w/v) and the chickpea beverage varied between 1.0% and 1.5% (w/v). The “milk” yield obtained for the optimized procedure B was 1221 g/100 g of dry seed and 1247 g/100 g of dry seed, for chickpea beverage and lupin beverage, respectively. Sensory results show that chickpea beverage with cooking water has the best taste. All pulses-based beverages are typical non-Newtonian fluids, similarly to current non-dairy alternative beverages. In this respect, the sprouted chickpea beverage, without the cooking water, presents the most pronounced shear-thinning behavior of all formulations.


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