scholarly journals Observations of bubbles in natural seep flares at MC 118 and GC 600 using in situ quantitative imaging

2016 ◽  
Vol 121 (4) ◽  
pp. 2203-2230 ◽  
Author(s):  
Binbin Wang ◽  
Scott A. Socolofsky ◽  
John A. Breier ◽  
Jeffrey S. Seewald
Keyword(s):  
Quantitative Imaging

Direct quantitative imaging of the writing stage in a photosensitive azopolymer by digital holography

Soft Matter ◽  
2019 ◽  
Vol 15 (39) ◽  
pp. 7809-7813 ◽  
Author(s):  
V. Pagliarulo ◽  
A. Calabuig ◽  
S. Grilli ◽  
P. Ferraro
Keyword(s):  
Thin Films ◽  
Real Time ◽  
Surface Relief ◽  
Digital Holography ◽  
Light Exposure ◽  
Quantitative Imaging ◽  
Continuous Light ◽  
Full Field ◽  
Surface Relief Grating

In this paper, we demonstrated that the gradual formation of a surface relief grating (SRG) in azopolymer thin films under continuous light exposure could be directly observed in situ and in real-time, allowing full-field characterization.

scholarly journals Quantitative Imaging of Biochemistry in Situ and at the Nanoscale

2020 ◽  
Vol 6 (11) ◽  
pp. 1938-1954
Author(s):  
Yamuna Krishnan ◽  
Junyi Zou ◽  
Maulik S. Jani
Keyword(s):  
Quantitative Imaging

scholarly journals In Situ Quantitative Imaging of Single-Molecule Co-Immunoprecipitation

2012 ◽  
Vol 102 (3) ◽  
pp. 600a
Author(s):  
Hong-Won Lee ◽  
Janghyun Yoo ◽  
Taeyoon Kyung ◽  
Tackhoon Kim ◽  
Changbong Hyeon ◽  
...  
Keyword(s):  
Single Molecule ◽  
Quantitative Imaging

scholarly journals Nucleosome stability measured in situ by automated quantitative imaging

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
László Imre ◽  
Zoltán Simándi ◽  
Attila Horváth ◽  
György Fenyőfalvi ◽  
Péter Nánási ◽  
...  
Keyword(s):  
Quantitative Imaging ◽  
Nucleosome Stability

scholarly journals Comprehensive histological imaging of native microbiota in human glioma

2021 ◽  
Author(s):  
Jiajia Zhao ◽  
Dian He ◽  
Hei Ming Lai ◽  
Yingying Xu ◽  
Yunhao Luo ◽  
...  
Keyword(s):  
Intercellular Space ◽  
Quantitative Imaging ◽  
Three Dimensional ◽  
Human Glioma ◽  
Microscopy Imaging ◽  
Nuclear Membranes ◽  
Tumor Tissues ◽  
Tumor Types ◽  
First Time

Abstract Mounting evidence suggests that distinct microbial communities reside in tumors and play important roles in tumor physiology. Recently, Nejman et al. profiled the composition and localization of intratumoral bacteria using 16S DNA sequencing and histological visualization methods across seven tumor types, including human glioblastoma. However, considering potential contamination in their sample origins and processing, the results based on traditional histological methods need to be validated. Here, we propose a three-dimensional (3D) intratumoral microbiota visualization and quantification protocol to observe microbiota in intact tumor tissues on the premise of avoiding possible contamination in the surface of tissues, based on tissue clearing, immunofluorescent labeling, microscopy imaging, and image processing. For the first time, we have achieved 3D quantitative imaging of bacterial LPS fluorescent signals deep in gliomas in a contamination-free manner, which was founded mostly localized near nuclear membranes or in the intercellular space. Through an automated statistical algorithm, reliable signals can be distinguished for further analysis of their sizes, distribution, and fluorescence intensities. Combining two-dimensional images from multiple thin-section histological methods, including immunochemistry and fluorescence in situ hybridization, we provide a comprehensive histological investigation of the morphology and distribution of these signals on human glioma samples. We expect that this multi-evidence chain will provide supporting proof for the presence of intratumoral bacteria in human glioma and that the integrated pipeline can be applied to investigate the native bacteria within diverse tumors and contribute to the interpretation of their direct roles in the tumor microenvironment.

scholarly journals In Situ Quantitative Imaging of Single-Molecule Co-Immunoprecipitation

2012 ◽  
Vol 102 (3) ◽  
pp. 180a
Author(s):  
Hong-Won Lee ◽  
Jang-Hyun Yoo ◽  
Byung-San Choi ◽  
Han-Ki Lee ◽  
Tae-Young Yoon
Keyword(s):  
Single Molecule ◽  
Quantitative Imaging

scholarly journals In situ quantitative imaging of cellular lipids using molecular sensors

2011 ◽  
Vol 3 (11) ◽  
pp. 868-874 ◽  
Author(s):  
Youngdae Yoon ◽  
Park J. Lee ◽  
Svetlana Kurilova ◽  
Wonhwa Cho
Keyword(s):  
Quantitative Imaging ◽  
Molecular Sensors ◽  
Cellular Lipids
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