Proteome Analysis of Oxidative Stress: Glutathionyl Hemoglobin in Diabetic and Uremic Patients

2006 ◽  
pp. 651-667 ◽  
Author(s):  
Toshimitsu Niwa
Keyword(s):  
Oxidative Stress ◽  
Proteome Analysis ◽  
Uremic Patients

Homocyst(e)ine, oxidative stress, and endothelium function in uremic patients

2001 ◽  
Vol 59 (s78) ◽  
pp. 243-245 ◽  
Author(s):  
Ziad A. Massy ◽  
Irene Ceballos ◽  
Bernadette Chadefaux-Vekemens ◽  
Thao Nguyen-Khoa ◽  
Beatrice Descamps-Latscha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelium Function ◽  
Uremic Patients

scholarly journals Elucidating the cellular response of silver nanoparticles as a potential combinatorial agent for cisplatin chemotherapy

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Renata Rank Miranda ◽  
Micaella Pereira da Fonseca ◽  
Barbara Korzeniowska ◽  
Lilian Skytte ◽  
Kaare Lund Rasmussen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Cellular Response ◽  
Proteome Analysis ◽  
Antioxidant Defense System ◽  
Normal Cells ◽  
Cancer Hallmarks ◽  
Antioxidant Proteins ◽  
Tumoral Cells

Abstract Background Combination chemotherapy uses drugs that target different cancer hallmarks, resulting in synergistic or additive toxicity. This strategy enhances therapeutic efficacy as well as minimizes drug resistance and side effects. In this study, we investigated whether silver nanoparticles act as a combinatorial partner to cisplatin. In so doing, we compared post-exposure biological endpoints, intracellular drug accumulation, and changes in the proteome profile of tumoral and normal cell lines. Results Combinatorial exposure corresponded to cytotoxicity and oxidative stress in both cell lines, yet was substantially more effective against tumoral cells. Proteome analysis revealed that proteins related to energy metabolism pathways were upregulated in both cell lines, suggesting that combinatorial exposure corresponded to energetic modulation. However, proteins and upstream regulators involved in the cell cycle were downregulated, indicating reduced cell proliferation. The response to oxidative stress was markedly different in both cell lines; downregulation of antioxidant proteins in tumoral cells, yet upregulation of the antioxidant defense system in normal cells. These outcomes may have avoided higher cell death rates in normal cells. Conclusions Taken together, our results indicate that combining silver nanoparticles with cisplatin increases the biological activity of the latter, and the combination warrants further exploration for future therapies.

scholarly journals Homocyst(e)ine, oxidative stress, and endothelium function in uremic patients

2001 ◽  
Vol 59 ◽  
pp. S243-S245 ◽  
Author(s):  
Ziad A. Massy ◽  
Irène Ceballos ◽  
Bernadette Chadefaux-Vekemens ◽  
Thao Nguyen-Khoa ◽  
Beatrice Descamps-Latscha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelium Function ◽  
Uremic Patients

scholarly journals Proteome analysis reveals roles of L-DOPA in response to oxidative stress in neurons

2014 ◽  
Vol 15 (1) ◽  
pp. 93 ◽  
Author(s):  
Mohammad-Saeid Jami ◽  
Ramavati Pal ◽  
Esthelle Hoedt ◽  
Thomas A Neubert ◽  
Jan Larsen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Proteome Analysis

scholarly journals Elucidating the cellular response of silver nanoparticles as a potential combinatorial agent for cisplatin chemotherapy

2020 ◽  
Author(s):  
Renata Rank Miranda ◽  
Micaella Pereira da Fonseca ◽  
Barbara Korzeniowska ◽  
Lilian Skytte ◽  
Kaare Lund Rasmussen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Cellular Response ◽  
Proteome Analysis ◽  
Antioxidant Defense System ◽  
Normal Cells ◽  
Cancer Hallmarks ◽  
Antioxidant Proteins ◽  
Tumoral Cells

Abstract Background: Combination chemotherapy uses drugs that target different cancer hallmarks, resulting in synergistic or additive toxicity. This strategy enhances therapeutic efficacy as well as minimizes drug resistance and side effects. In this study, we investigated whether silver nanoparticles act as a combinatorial partner to cisplatin. In so doing, we compared post-exposure biological endpoints, intracellular drug accumulation, and changes in the proteome profile of tumoral and normal cell lines. Results: Combinatorial exposure corresponded to cytotoxicity and oxidative stress in both cell lines, yet was substantially more effective against tumoral cells. Proteome analysis revealed that proteins related to energy metabolism pathways were upregulated in both cell lines, suggesting that combinatorial exposure corresponded to energetic modulation. However, proteins and upstream regulators involved in the cell cycle were downregulated, indicating reduced cell proliferation. The response to oxidative stress was markedly different in both cell lines; downregulation of antioxidant proteins in tumoral cells, yet upregulation of the antioxidant defense system in normal cells. These outcomes may have avoided higher cell death rates in normal cells. Conclusions: Taken together, our results indicate that combining silver nanoparticles with cisplatin increases the biological activity of the latter, and the combination warrants further exploration for future therapies.

scholarly journals Proteome Analysis of Whole-Body Responses in Medaka Experimentally Exposed to Fish-Killing Dinoflagellate Karenia mikimotoi

2021 ◽  
Vol 22 (21) ◽  
pp. 11625
Author(s):  
Celia Sze-Nga Kwok ◽  
Kaze King-Yip Lai ◽  
Winnie Lam ◽  
Steven Jing-Liang Xu ◽  
Sai-Wo Lam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Responses ◽  
Time Course ◽  
Harmful Algal Bloom ◽  
Proteome Analysis ◽  
Whole Body ◽  
Marine Medaka ◽  
Two Dimensional Gel Electrophoresis ◽  
Karenia Mikimotoi ◽  
Part Time

Karenia mikimotoi is a well-known harmful algal bloom species. Blooms of this dinoflagellate have become a serious threat to marine life, including fish, shellfish, and zooplanktons and are usually associated with massive fish death. Despite the discovery of several toxins such as gymnocins and gymnodimines in K. mikimotoi, the mechanisms underlying the ichthyotoxicity of this species remain unclear, and molecular studies on this topic have never been reported. The present study investigates the fish-killing mechanisms of K. mikimotoi through comparative proteomic analysis. Marine medaka, a model fish organism, was exposed to K. mikimotoi for a three-part time period (LT25, LT50 and LT90). Proteins extracted from the whole fish were separated by using two-dimensional gel electrophoresis, and differentially expressed proteins were identified with reference to an untreated control. The change in fish proteomes over the time-course of exposure were analyzed. A total of 35 differential protein spots covering 19 different proteins were identified, of which most began to show significant change in expression levels at the earliest stage of intoxication. Among the 19 identified proteins, some are closely related to the oxidative stress responses, energy metabolism, and muscle contraction. We propose that oxidative stress-mediated muscle damage might explain the symptoms developed during the ichthyotoxicity test, such as gasping for breath, loss of balance, and body twitching. Our findings lay the foundations for more in-depth studies of the mechanisms of K. mikimotoi’s ichthyotoxicity.

scholarly journals Proteome analysis of myocardium from chronic Chagas’ disease patients: identification of immune response, oxidative stress, apoptosis, and metabolism proteins

2006 ◽  
Vol 20 (5) ◽  
Author(s):  
Priscila Camillo Teixeira ◽  
Leo Kei Iwai ◽  
Ronaldo Honorato ◽  
Alfredo Fiorelli ◽  
Noedir Stolf ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Immune Response ◽  
Chagas Disease ◽  
Proteome Analysis ◽  
Chronic Chagas Disease

Supplementation of Emblica Officinalis (Amla) Extract Reduces Oxidative Stress in Uremic Patients

2009 ◽  
Vol 37 (01) ◽  
pp. 19-25 ◽  
Author(s):  
Tung-Sheng Chen ◽  
Show-Yih Liou ◽  
Yen-Lin Chang
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Total Antioxidant Status ◽  
Atherogenic Index ◽  
Emblica Officinalis ◽  
Antioxidant Power ◽  
Uremic Patients ◽  
Physiological Benefits ◽  
High Level ◽  
Plasma Antioxidant

Emblica Officinalis (also known as Amla or Indian Gooseberry), a natural, traditional and functional food in Asia, has physiological benefits such as hepato-, cyto- and radio- protection, as well as hypolipidemic effects. In addition, Amla often functions as a potent antioxidant due to the high level of ascorbic acid (ranging from 1,100 to 1,700 mg/100 g of fruit) in its fruit. The aim of this study was to determine whether supplementation with Amla extract could reduce oxidative stress in patients with uremia. The findings show that supplementation with Amla extract for 4 months reduced the plasma oxidative marker, 8-iso-prostaglandin, (M0 vs. M4 = 1415 ± 1234 pg/ml vs. 750 ± 496 pg/ml, p < 0.05) and increased plasma total antioxidant status (TAS) (M0 vs. M4 = 2.32 ± 0.14 mM vs. 2.55 ± 0.24 mM, p < 0.05) in uremic patients. On the other hand, there were no significant differences observed in liver function (GOP and GPT), renal function (creatinine, blood urea nitrogen and uric acid), diabetic index (plasma glucose and adiponectin) and atherogenic index (LDL/HDL ratio, total cholesterol and homocysteine) in patients treated with Amla for 4 months. Our data suggest that Amla supplementation may increase plasma antioxidant power and decrease oxidative stress in uremic patients. However, Amla extract did not influence hepatic or renal function, or diabetic and atherogenic indices in uremic patients.

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