Genetic Diversity and Association Analyses of Canadian Maize Inbred Lines with Agronomic Traits and Simple Sequence Repeat Markers

2018 ◽  
Vol 6 (2) ◽  
pp. 159-169 ◽  
Author(s):  
Kyu Jin Sa ◽  
Tak Ki Hong ◽  
Ju Kyong Lee
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Agronomic Traits ◽  
Inbred Lines ◽  
Sequence Repeat ◽  
Association Analyses ◽  
Simple Sequence Repeat Markers ◽  
Maize Inbred Lines ◽  
Simple Sequence

Genetic diversity analysis of 119 Canadian maize inbred lines based on pedigree and simple sequence repeat markers

2011 ◽  
Vol 91 (4) ◽  
pp. 651-661 ◽  
Author(s):  
L. M. Reid ◽  
K. Xiang ◽  
X. Zhu ◽  
B. R. Baum ◽  
S. J. Molnar
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Inbred Lines ◽  
Diversity Analysis ◽  
Sequence Repeat ◽  
Heterotic Groups ◽  
Simple Sequence Repeat Markers ◽  
Genetic Diversity Analysis ◽  
Maize Inbred Lines ◽  
Simple Sequence

Reid, L. M., Xiang, K., Zhu, X., Baum, B. R. and Molnar, S. J. 2011. Genetic diversity analysis of 119 Canadian maize inbred lines based on pedigree and simple sequence repeat markers. Can. J. Plant Sci. 91: 651–661. Since the early 1920s Agriculture and Agri-Food Canada (AAFC) has been developing maize varieties and inbred lines adapted to the early maize growing regions. These inbreds represent a large group of genetically diverse genotypes; however, many of the inbreds are of unknown heterotic backgrounds. The purpose of this study was to classify 119 elite maize inbred lines released from AAFC into heterotic groups using both pedigree data and simple sequence repeat (SSR) markers and also to explore the consistency among different classification analyses. Pedigree analysis placed the inbred lines into eight groups, six of which corresponded to known major heterotic groups representing Iowa Stiff Stalk Synthetic (BSSS), European flint, Lancaster, Minnesota 13, Early Butler, and Iodent; the two remaining groups consisted of germplasm derived mostly from Pioneer 3990 or Pioneer 3994 sources. Simple sequence repeat analysis of 105 loci resulted in a clustering of the inbreds into 10 groups. In comparison with the grouping based on pedigree, the SSR clustering groups had some discrepancies and groups of genetically similar germplasm, based on pedigree, could not always be confirmed with molecular markers. The results of this study will allow researchers and maize breeders to make more informed decisions on the use of these inbreds in breeding programs.

scholarly journals Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers

2014 ◽  
Vol 13 (3) ◽  
pp. 7852-7863 ◽  
Author(s):  
R.F. Peixoto-Junior ◽  
S. Creste ◽  
M.G.A. Landell ◽  
D.S. Nunes ◽  
A. Sanguino ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Puccinia Melanocephala ◽  
Simple Sequence

scholarly journals Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers

2019 ◽  
Vol 20 (8) ◽  
Author(s):  
Ni Luh Arpiwi ◽  
I Gusti Ayu Sugi Wahyuni ◽  
I Ketut Muksin
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Pongamia Pinnata ◽  
Sequence Repeat ◽  
Binary Matrix ◽  
Simple Sequence Repeat Markers ◽  
Group I ◽  
Simple Sequence

Abstract. Arpiwi NL, Wahyuni IGAS, Muksin IK. 2019. Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers. Biodiversitas 20: 2134-2142. Pongamia pinnata (L.) Pierre is a member of family Leguminosae that produces seed oil for biodiesel feedstock. The aim of the present study was to determine genetic diversity of pongamia trees that grow in Bali using Inter Simple Sequence Repeat (ISSR) markers. This study is important to support the breeding program for the improvement of the biodiesel producing species. Leaf samples were taken from 26 pongamia trees grown on northern and southern coastal areas of Bali. Genomic DNA was isolated from fresh leaves sample and was amplified by Polymerase Chain Reaction (PCR) using 9 ISSR primers. The banding patterns of DNA after PCR were scored and tabulated into a binary matrix. Genetic distance was generated by pairwise distance using composite maximum likelihood. A dendrogram was constructed using Unweighted Pair Group Method Arithmetic (UPGMA) method. The binary matrix was further analyzed for Nonmetric Multidimensional Scaling (NMDS) with Primer E V.6 software. DNA concentrations ranged from 98.59-100.55 ng/μL with sufficient quality for PCR. The number of alleles for 9 primers was 43, the number of the polymorphic band was 35, and the number of monomorphic bands was 8. Percentage of polymorphism ranged from 50 to 100%. Cluster analysis of 26 DNA of pongamia trees showed that the trees were grouped into two, namely group I and II. Group I consisted of two trees only, namely Uma Anyar 1 and Penarukan 1. Group II consisted of 24 pongamia trees which were divided into 3 subgroups, namely IIA, IIB, and IIC with close genetic distance. Analysis of NMDS supported cluster analysis that 23 out of 26 pongamia trees had close genetic distance, and possibly they come from a similar source. Genetic diversity of pongamia in Bali needs to be widen possibly by the introduction of new planting materials from across Indonesia or seed procurement from different sources.

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