scholarly journals Crossbreeding of cry1Ac/bar Transgenic Progeny with a Non-transgenic Elite Rice Cultivar for Minimizing the Underlying Grain Cost of Bt Transgenic Rice

2015 ◽  
Vol 3 (1) ◽  
pp. 21-29
Author(s):  
Mi-Ok Woo ◽  
Jin-Hyoung Lee ◽  
Seonghyu Shin ◽  
Kong-Sik Shin ◽  
Hee-Jong Koh ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Rice Cultivar ◽  
Transgenic Progeny ◽  
Bt Transgenic Rice

Cry1Ab protein from Bt transgenic rice does not residue in rhizosphere soil

2006 ◽  
Vol 143 (3) ◽  
pp. 449-455 ◽  
Author(s):  
Haiyan Wang ◽  
Qingfu Ye ◽  
Wei Wang ◽  
Licheng Wu ◽  
Weixiang Wu
Keyword(s):  
Transgenic Rice ◽  
Rhizosphere Soil ◽  
Cry1ab Protein ◽  
Bt Transgenic Rice

Determination of the movement and persistence of Cry1Ab/1Ac protein released from Bt transgenic rice under field and hydroponic conditions

2013 ◽  
Vol 58 ◽  
pp. 107-114 ◽  
Author(s):  
Yongmo Wang ◽  
Huawei Hu ◽  
Jiacheng Huang ◽  
Jianhong Li ◽  
Biao Liu ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Hydroponic Conditions ◽  
Bt Transgenic Rice

Modified accumulation of selected heavy metals in Bt transgenic rice

2009 ◽  
Vol 21 (11) ◽  
pp. 1607-1612 ◽  
Author(s):  
Haiyan WANG ◽  
Jianzhong HUANG ◽  
Qingfu YE ◽  
Dianxing WU ◽  
Ziyuan CHEN
Keyword(s):  
Heavy Metals ◽  
Transgenic Rice ◽  
Bt Transgenic Rice

No effect of Bt- transgenic rice litter on the meiobenthos community in field ditches

2016 ◽  
Vol 73 (6) ◽  
pp. 1213-1219 ◽  
Author(s):  
Yongbo Liu ◽  
Wanxiang Jiang ◽  
Yuyong Liang ◽  
Caiyun Zhao ◽  
Junsheng Li
Keyword(s):  
Transgenic Rice ◽  
Bt Transgenic Rice

scholarly journals Inheritance of NmDef02 synthetic defensin gene and hpt selectable marker in four successive generations of transgenic rice (Oryza sativa, cv. J-104)

2021 ◽  
Vol 8 (9) ◽  
pp. 34-41
Author(s):  
Maylin Pérez-Bernal ◽  
Magali Delgado ◽  
Daymí Abreu ◽  
Onel Valdivia ◽  
Raúl Armas
Keyword(s):  
Transgenic Rice ◽  
Selectable Marker ◽  
Marker Gene ◽  
Rice Cultivar ◽  
Future Research ◽  
Maturation Stage ◽  
Defensin Gene ◽  
Regenerated Plants ◽  
Marker Free ◽  
Successive Generations

The evaluation of the inheritance and stability of the transgenes is essential for the application of transgenic plants in agriculture. In this work, we studied the inheritance of two transgenes in T1, T2, T3 and T4 rice progenies. Transgenic rice plants (cv. J-104) was obtained by biolistic method using a synthetic defensin gene (NmDef02) and hpt as selectable marker gene, co-transformed in a 4:1 proportion, respectively. Regenerated plants were acclimated under natural conditions. The study started from the primary transformants that fulfilled the agronomic characters reported by the experts for the J-104 rice cultivar in the maturation stage. The integration and relative expression of NmDef02 in T1 plants was verified by Southern blot and qRT-PCR, respectively. The inheritance of transgenes over four generations was analyzed by PCR. The following transgene combinations were identified: NmDef02(+)hpt(+), NmDef02(+)hpt(-) and NmDef02(-)hpt(-). The most advantageous combination was NmDef02(+)hpt(-), which corresponded to the marker-free plants harboring the gene of interest. The inheritance of NmDef02 was confirmed in T1 and T2 progenies, but in some T3 and T4 lines the loss of this gene was verified. This inheritance analysis provided information concerning the transgenic population, but the mechanisms that destabilize the inheritance of the gene of interest will be the goal of future research.

Engineering of Bt Transgenic Rice for Insect Pest Protection

2003 ◽  
Vol 5 (2-3) ◽  
pp. 77-91 ◽  
Author(s):  
S.K. Datta ◽  
G. Chandel ◽  
J. Tu ◽  
N. Baisakh ◽  
K. Datta
Keyword(s):  
Transgenic Rice ◽  
Insect Pest ◽  
Bt Transgenic Rice

Overexpression of sedoheptulose-1,7-bisphosphatase enhances photosynthesis and growth under salt stress in transgenic rice plants

2007 ◽  
Vol 34 (9) ◽  
pp. 822 ◽  
Author(s):  
Lingling Feng ◽  
Yujun Han ◽  
Gai Liu ◽  
Baoguang An ◽  
Jing Yang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Transgenic Plants ◽  
Transgenic Rice ◽  
Calvin Cycle ◽  
Rice Cultivar ◽  
Wild Type ◽  
Bisphosphate Carboxylase ◽  
Rice Plants ◽  
Transgenic Rice Plants

Activity of the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (SBPase; EC3.1.3.37) was increased in the transgenic rice cultivar zhonghua11 (Oryza sativa L. ssp. japonica) by overexpressing OsSbp cDNA from the rice cultivar 9311 (Oryza sativa ssp. indica). This genetic engineering enabled the transgenic plants to accumulate SBPase in chloroplasts and resulted in enhanced tolerance of transgenic rice plants to salt stress at the young seedlings stage. Moreover, CO2 assimilation in transgenic rice plants was significantly more tolerant to salt stress than in wild-type plants. The analysis of chlorophyll fluorescence and the activity of SBPase indicated that the enhancement of photosynthesis in salt stress was not related to the function of PSII but to the activity of SBPase. Western-blot analysis showed that salt stress led to the association of SBPase with the thylakoid membranes from the stroma fractions. However, this association was much more prominent in wild-type plants than in transgenic plants. Results suggested that under salt stress, SBPase maintained the activation of ribulose-1,5-bisphosphate carboxylase-oxygenase by providing more regeneration of the acceptor molecule ribulose-1,5-bisphosphate in the soluble stroma and by preventing the sequestration of Rubisco activase to the thylakoid membrane from the soluble stroma, and, thus, enhanced the tolerance of photosynthesis to salt stress. Results suggested that overexpression of SBPase was an effective method for enhanncing salt tolerance in rice.

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