scholarly journals Transcriptional Profiling of Soft-rot Resistant Transgenic Chinese Cabbage (Brassica rapa L.) Constitutively Overexpressing a Human Cathelicidin Antimicrobial Peptide (hCAP18/LL-37)

2013 ◽  
Vol 1 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Yu-Jin Jung
Keyword(s):  
Antimicrobial Peptide ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Transcriptional Profiling ◽  
Soft Rot ◽  
Transgenic Chinese Cabbage

Enhancement of tolerance to soft rot disease in the transgenic Chinese cabbage (Brassica rapa L. ssp. pekinensis) inbred line, Kenshin

2009 ◽  
Vol 28 (10) ◽  
pp. 1581-1591 ◽  
Author(s):  
Enkhchimeg Vanjildorj ◽  
Seo Young Song ◽  
Zhi Hong Yang ◽  
Jae Eul Choi ◽  
Yoo Sun Noh ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Inbred Line ◽  
Chinese Cabbage ◽  
Soft Rot ◽  
Soft Rot Disease ◽  
Rot Disease ◽  
Transgenic Chinese Cabbage

scholarly journals DNA Methylation Level Changes in Transgenic Chinese Cabbage (Brassica rapa ssp. pekinensis) Plants and Their Effects on Corresponding Gene Expression Patterns

Genes ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1563
Author(s):  
Jee-Soo Park ◽  
Yun-Hee Shin ◽  
Young-Doo Park
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Methylation Level ◽  
Bisulfite Sequencing ◽  
Expression Patterns ◽  
Methylation Status ◽  
Transgenic Chinese Cabbage

Plant tissue culture is an in vitro technique used to manipulate cells, tissues, or organs, and plays an important role in genetic transformation. However, plants cultured in vitro often exhibit unintended genetic and epigenetic variations. Since it is important to secure the stability of endogenous and exogenous gene expressions in transgenic plants, it is preferable to avoid the occurrence of such variations. In this study, we focused on epigenetic variations, exclusively on methylation level changes of DNA, in transgenic Chinese cabbage (Brassica rapa ssp. pekinensis) plants. To detect these methylation level changes of DNA, bisulfite sequencing was performed and the obtained sequences were compared with the ‘CT001’ reference genome. Differentially methylated regions (DMRs) of DNA between the non-transgenic and transgenic lines were detected by bisulfite sequencing, and ten DMRs located in exonic regions were identified. The regions with methylation variations that were inherited and consistently maintained in the next generation lines were selected and validated. We also analyzed the relationship between methylation status and expression levels of transformant-conserved DMR (TCD) genes by quantitative reverse transcription-PCR. These results suggested that the changes in methylation levels of these DMRs might have been related to the plant transformation process, affecting subsequent gene expression. Our findings can be used in fundamental research on methylation variations in transgenic plants and suggest that these variations affect the expression of the associated genes.

HETEROLOGOUS EXPRESSION OF ANTIMICROBIAL PEPTIDE LL-37 IN CHINESE CABBAGE WITH ENHANCED RESISTANCE TO PATHOGENS

2015 ◽  
Vol 13 (2) ◽  
pp. 124-130
Author(s):  
Bayartbat Ishvaanjil ◽  
Yu-Jin Jung ◽  
Uyangaa Temuujin ◽  
Soon-Youl Lee ◽  
Kwon-Kyoo Kang
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Western Blot ◽  
Antimicrobial Peptide ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Blot Analysis ◽  
Wide Spectrum ◽  
Intron Sequence ◽  
Rt Pcr ◽  
Enhanced Resistance

The human antimicrobial peptide, LL-37 gene was overexpressed in Chinese cabbage ‘Osome’ (Brassica rapa) by Agrobacterium tumefaciens-mediated transformation. In order to increase the expression of the antimicrobial peptide, we used RolA intron sequence in front of the LL-37peptide gene. We confirmed the expression of LL-37 in cabbage by RT-PCR and Western Blot analysis. Four transgenic T1 plants were confirmed that LL-37 was expressed. Cabbages expressing the humanLL-37 gene were challenged by various plant pathogen. Transgenic cabbage plants overproducing human LL-37 are expected to possess a durable and wide-spectrum resistance against various pathogens.Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 124-130

scholarly journals Comprehensive Analysis of the Histone Deacetylase Gene Family in Chinese Cabbage (Brassica rapa): From Evolution and Expression Pattern to Functional Analysis of BraHDA3

Agriculture ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 244
Author(s):  
Seung Hee Eom ◽  
Tae Kyung Hyun
Keyword(s):  
Functional Analysis ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Stress Responses ◽  
Histone Deacetylases ◽  
Expression Patterns ◽  
Evolutionary Relationship ◽  
Gene Families ◽  
Physiological Processes ◽  
Lysine Residues

Histone deacetylases (HDACs) are known as erasers that remove acetyl groups from lysine residues in histones. Although plant HDACs play essential roles in physiological processes, including various stress responses, our knowledge concerning HDAC gene families and their evolutionary relationship remains limited. In Brassica rapa genome, we identified 20 HDAC genes, which are divided into three major groups: RPD3/HDA1, HD2, and SIR2 families. In addition, seven pairs of segmental duplicated paralogs and one pair of tandem duplicated paralogs were identified in the B. rapa HDAC (BraHDAC) family, indicating that segmental duplication is predominant for the expansion of the BraHDAC genes. The expression patterns of paralogous gene pairs suggest a divergence in the function of BraHDACs under various stress conditions. Furthermore, we suggested that BraHDA3 (homologous of Arabidopsis HDA14) encodes the functional HDAC enzyme, which can be inhibited by Class I/II HDAC inhibitor SAHA. As a first step toward understanding the epigenetic responses to environmental stresses in Chinese cabbage, our results provide a solid foundation for functional analysis of the BraHDAC family.

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