Use of Elisa for Preliminary Screening of 19 Nortestosterone Anabolic Steroid in Cattle Meat in Republic of Macedonia

SummarySix lyophilized plasma samples were sent to 20 “expert” laboratories for assessment of lupus anticoagulant (LA). Four samples contained pooled LA of graded potency mixed with aged normal plasma. One contained LA plus cephalin phospholipid and one contained a nonspecific venom anticoagulant. Sixteen methods were used overall with some participants using up to 8 methods. Results were scored in regard to the known potencies of LA in the samples and other known induced defects.Activated partial thromboplastin time (APTT) tests used by most participants for preliminary screening were relatively sensitive, but non-specific. Platelet or phospholipid neutralization procedures (PNP) appeared to be sensitive and specific but showed a non-linear response to increased LA content. Kaolin clotting time (KCT) tests showed the most sensitive response to increased LA content but the weaker LA were not scored as abnormal by most laboratories as the samples may have contained platelet fragments. Other commonly used tests such as the tissue thromboplastin inhibition (TTI) test and the dilute Russell’s viper venom test (DRVVT) were carried out somewhat inconsistently. The variability in performance of tests in different laboratories indicates that standardization of methodology is urgently required.Generally it seemed that most clotting tests were “bypassed” by the addition of phospholipid to a known LA-positive sample in apparently direct proportion to their sensitivity. Sample preparation, especially prevention of contamination with activated platelets is a vital preliminary part in the assay of LA.

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Plasma Fibrinolytic Activity Following Oral Anabolic Steroid Therapy

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651371 ◽

1975 ◽

Vol 34 (01) ◽

pp. 236-245 ◽

Cited By ~ 3

Author(s):

I. D Walker ◽

J. F Davidson ◽

P Young ◽

J. A Conkie

Keyword(s):

Heart Disease ◽

Ischaemic Heart Disease ◽

Anabolic Steroid ◽

Fibrinolytic Activity ◽

Anabolic Steroids ◽

Long Term Effects ◽

Lysis Time ◽

Detailed Evaluation ◽

Euglobulin Lysis Time

SummarySix anabolic steroids were assessed for their ability to enhance plasma fibrinolytic activity in males with ischaemic heart disease. Five 17α-alkylated steroids (Ethyloestrenol, Norethandrolone, Methandienone, Methylandrostenediol and Oxymetholone) were examined and all produced a significant increase in plasma plasminogen activator as measured by the euglobulin lysis time. The only non-17α-alkylated steroid studied (Methenolone acetate) failed to enhance fibrinolysis. The 17α-alkylated steroids studied all deserve more detailed evaluation of their long term effects on plasma fibrinolytic activity.

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The importance of recognition of the Republic of Kosovo by the Republic of Macedonia for both states

10.33107/ubt-ic.2018.408 ◽

2018 ◽

Author(s):

Gurakuq Kuci ◽

Bejtush Gashi

Keyword(s):

Republic Of Macedonia ◽

The Republic

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OXYDATION DES ANABOLIKUMS 1-METHYL-ANDROST-1-EN-17β-OL-3-ON MIT WASSERSTOFFTRANSFER AUF ÖSTRON

Acta Endocrinologica ◽

10.1530/acta.0.0670517 ◽

1971 ◽

Vol 67 (3) ◽

pp. 517-530 ◽

Cited By ~ 1

Author(s):

Martin Wenzel

Keyword(s):

Rat Liver ◽

Anabolic Steroid ◽

Female Rats ◽

Male Rats ◽

Female Rat ◽

Liver Slices ◽

Androgen Effects ◽

Biochemical Difference

ABSTRACT With the aid of metenolon-17α-T a tritium-transfer to oestrone in rat liver slices was demonstrated. This tritium-transfer from metenolon17α-T to oestrone yielding tritium-labelled oestradiol had a higher efficiency in male than in female rat liver. Correspondingly in the presence of metenolon the relation of oestrone to oestradiol is changed more in male than in female rat liver. Looking for biochemical differences between the anabolic steroid metenolon and testosterone the oxydation at C17 was measured in different organs of the rat using 17α-T-labelled steroids. The highest oxydation rate was found for both steroids in the liver. In the sexual organs of male rats the oxydation rate of testosterone was 50–10 times higher than that of the anabolic steroid. This difference was less in sexual organs of female rats. This result of a greater biochemical difference between both steroids in males than in females leads to the question, whether the dissociation between the anabolic and the androgen effects is higher in males than in females.

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