scholarly journals Investigation of Yersinia ruckeri Infection in Rainbow Trout (Oncorhynchus mykiss Walbaum 1792) Farms by Polymerase Chain Reaction (PCR) and Bacteriological Culture

2012 ◽  
Author(s):  
Engin ŞEKER ◽  
Murat KARAHAN ◽  
Ünal İSPİR ◽  
Burhan ÇETİNKAYA ◽  
Naim SAĞLAM ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Yersinia Ruckeri ◽  
Chain Reaction ◽  
Bacteriological Culture ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Polymerase Chain

Identification of Atlantic Salmon (Salmo salar) and Rainbow Trout (Oncorhynchus mykiss) by Using Polymerase Chain Reaction Amplification and Restriction Analysis of the Mitochondrial Cytochrome b Gene

1998 ◽  
Vol 61 (4) ◽  
pp. 482-486 ◽  
Author(s):  
ESTHER CARRERA ◽  
TERESA GARCÍA ◽  
ANA CÉSPEDES ◽  
ISABEL GONZÁLEZ ◽  
BERNABÉ SANZ ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rainbow Trout ◽  
Atlantic Salmon ◽  
Oncorhynchus Mykiss ◽  
Cytochrome B ◽  
Salmo Salar ◽  
Cytochrome B Gene ◽  
Chain Reaction ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Polymerase Chain

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the identification of fresh and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Digestion of the 359-bp PCR product with the endonucleases EcoRV and TaqI yielded specific banding patterns for salmon and trout. This genetic marker can be very useful for detecting fraudulent substitution of the cheaper smoked trout for the more expensive smoked salmon.

Differential Expression of the Dmrt1 Gene from Ovaries of Diploid and Triploid Rainbow Trout (Oncorhynchus mykiss)

2013 ◽  
Vol 634-638 ◽  
pp. 934-938 ◽  
Author(s):  
Ying Han ◽  
Kun Wang ◽  
Hai Long Li ◽  
Dana X Gao
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Differential Expression ◽  
Quantitative Polymerase Chain Reaction ◽  
Relative Difference ◽  
Molecular Evidence ◽  
Chain Reaction ◽  
Dmrt1 Gene ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Polymerase Chain

Reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) was used to determine the differential expression of the Dmrt1 gene in ovaries of diploid and triploid rainbow trout (Oncorhynchus mykiss) 10 and 20 months post-hatch. At 10 months post-hatch there was little relative difference in the expression of the Dmrt1 gene between triploid and diploid trout. However, at 20 months the expression of the Dmrt1 gene in triploids was more than 14 times higher than in diploids. Dmrt1 expression from 10 to 20 months suggests masculinization of the ovaries, which may be virilescent in triploid females at the later development phase (20 months). This study provides molecular evidence supporting the general claims that ovaries of female triploid rainbow trout do not develop normally resulting in sterility.

Sign in / Sign up

Export Citation Format

Share Document