scholarly journals Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) RTFS’ye (Rainbow trout Fry Syndrome) Neden Olan Flavobacterium psychrophilum Etkeninin Izolasyonu ve Antibakteriyel Sağaltım Seçeneğinin Belirlenmesi

2009 ◽  
Author(s):  
Murat BOYACIOGLU ◽  
Ferda AKAR
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Flavobacterium Psychrophilum ◽  
Rainbow Trout Fry Syndrome

Intramuscular challenge of rainbow trout (Oncorhynchus mykiss) with two Norwegian field strains of Flavobacterium psychrophilum

2013 ◽  
Vol 35 (2) ◽  
pp. 595-598 ◽  
Author(s):  
Børge N. Fredriksen ◽  
Anette Furevik ◽  
David Gauthier ◽  
Marie Egenberg ◽  
Erik D. Paulsen ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Flavobacterium Psychrophilum ◽  
Rainbow Trout Oncorhynchus Mykiss

Identification and expression of a host-recognized antigen, FspA, from Flavobacterium psychrophilum

Microbiology ◽  
2005 ◽  
Vol 151 (9) ◽  
pp. 3127-3135 ◽  
Author(s):  
Elizabeth M. Crump ◽  
Ján Burian ◽  
Philippe D. Allen ◽  
William W. Kay
Keyword(s):  
Rainbow Trout ◽  
Yersinia Pseudotuberculosis ◽  
Signal Sequence ◽  
Pcr Primers ◽  
Specific Protein ◽  
Two Dimensional Gel Electrophoresis ◽  
Flavobacterium Psychrophilum ◽  
Flavobacterium Johnsoniae ◽  
Vaccine Candidate Antigen ◽  
Rainbow Trout Fry Syndrome

Flavobacterium psychrophilum is the aetiological agent of rainbow trout fry syndrome, an economically important disease of immature salmonid fish for which there is no vaccine. Convalescent serum from the host, rainbow trout (Oncorhynchus mykiss), reacted strongly with a ∼20 kDa, Flavobacterium-specific protein antigen (subsequently named FspA) from F. psychrophilum. Protein-enriched, detergent-partitioned samples were separated by two-dimensional gel electrophoresis and the protein target was excised, proteolytically cleaved and the resulting peptides analysed by MS. Quadrupole-time-of-flight MS was used to generate a fragmented peptide spectrum. The resulting peptide sequences were then used to design degenerate PCR primers to amplify the gene (fspA) of interest: 612 bp encoding 203 aa, including a putative 19 aa N-terminal signal sequence which predicted a processed 19 303·6 Da protein. FspA proved to be unique and only homologous to two unspecified sequences reported from Flavobacterium johnsoniae, although weakly homologous to a Yersinia pseudotuberculosis adhesin. An amplified gene fragment (537 bp, encoding 179 aa) was further cloned into an expression vector, expressed as a ∼30 kDa N-terminal fusion protein and found to retain its strong reactivity with host serum antibodies. These results suggest that the surface-localized FspA may be an important subunit vaccine candidate antigen against F. psychrophilum.

Transcriptomic response of rainbow trout (Oncorhynchus mykiss) skeletal muscle to Flavobacterium psychrophilum

2019 ◽  
Vol 31 ◽  
pp. 100596
Author(s):  
Andrea Rivas-Aravena ◽  
Marcia Fuentes-Valenzuela ◽  
Sebastian Escobar-Aguirre ◽  
Cristian Gallardo-Escarate ◽  
Alfredo Molina ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Transcriptomic Response ◽  
Flavobacterium Psychrophilum ◽  
Rainbow Trout Oncorhynchus Mykiss
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