In vitro Rapid Regeneration of Betel Vine (Piper betle L.)

2017 ◽  
Vol 16 (3) ◽  
pp. 1-11
Author(s):  
Md Elahi ◽  
Homayra Huq ◽  
M Hoque ◽  
Fahima Khatun
Keyword(s):  
Piper Betle ◽  
Rapid Regeneration

scholarly journals In vitro toxin production by Fusarium solani f. sp. piperis

2003 ◽  
Vol 28 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Maria de Lourdes R. Duarte ◽  
Simon A. Archer
Keyword(s):  
Fusarium Solani ◽  
Biological Response ◽  
Black Pepper ◽  
Biologically Active ◽  
Piper Nigrum ◽  
Piper Betle ◽  
Toxic Metabolites ◽  
Culture Filtrates ◽  
Detached Leaves

Fusarium solani f. sp. piperis (teleomorph: Nectria haematococca f. sp. piperis), causal agent of root rot and stem blight on black pepper (Piper nigrum), produces secondary metabolites with toxigenic properties, capable of inducing vein discoloration in detached leaves and wilting in transpiring microcuttings. Production of F. solani f. sp. piperis (Fsp) toxic metabolites reached a peak after 25 days of static incubation on potato sucrose broth at 25 ºC under illumination. Changes in the pH of the culture filtrate did not alter the effect of toxic metabolites. However, when the pH was changed before the medium had been autoclaved, a more intense biological response was observed, with an optimum at pH 6.0. Isolates that produced red pigments in liquid cultures were more efficient in producing biologically active culture filtrates than those which produced pink coloured or clear filtrates suggesting that these pigments could be related to toxigenic activity. Detached leaves of seven black pepper cultivars and Piper betle showed symptoms of vein discoloration after immersion in autoclaved and non-autoclaved Fsp culture filtrates indicating the thermostable nature of these toxic metabolites.

scholarly journals Analisa Aktivitas Antibakteri Rebusan Daun Sirih Dengan Rebusan Daun Kemangi Terhadap Pertumbuhan Bakteri Streptococcus mutans

2020 ◽  
Vol 18 (1) ◽  
pp. 36
Author(s):  
ENNY WILLIANTI ◽  
THEODORA THEODORA ◽  
WAHYUNI DYAH PARMASARI
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Observational Research ◽  
Ocimum Sanctum ◽  
Piper Betle ◽  
Oral Flora ◽  
Betel Leaf ◽  
Growth Of Bacteria

<p><strong>ABSTRACT</strong><strong></strong></p><p><strong> </strong></p><p><strong>Background</strong>: Betel leaf contains essential oils consisting of bethelphenol, kavikol, sesquiterpenes, hydroxycavikol, cavibetol, estragol, eugenol and carvacrol. Essential oils are antibacterial due to the presence of phenol compounds and their derivatives that can denature the bacterial cell proteins. Basil leaves contain compounds from essential oils, namely 1,8-cineole, ß-bisabolene, and methyl eugenol. These three ingredients are soluble to ethanol and can cause damage to the cell membranes of the Streptococcus mutans bacteria, which are members of the normal oral flora but can turn into pathogens if the balance of normal flora is disturbed. The aim of this study was to determine the difference in the activity of the antibacterial  of decoction betel leaf (piper betle L. ) with a decoction of basil leaves (ocimum sanctum) against growth of bacteria <em>Streptococcus mutans</em> (in vitro study).</p><p><strong>M</strong><strong>ethod:</strong> this observational research with disk diffusion techniques. This study observed and measured the diameter of the inhibitory zone in MHA formed by decoction of betel leaf (piper betle L) and basil leaf (ocimum sanctum) in units of millimeters (mm). There were 2 groups with 16 replications.</p><p><strong>R</strong><strong>esults</strong>: the results of the description test showed that the antibacterial activity of the betel leaf decoction and the highest decoction of basil leaf was 17 mm and the lowest was 15 mm, but the average antibacterial value of betel leaf decoction (15,81) greater than the average value of antibacterial activity of basil leaf (15.75). This is because there are chemicals contained in betel leaf similar as contained in basil leaf, namely essential oils.</p><p><strong>Conclusion</strong>: there is no difference in the antibacterial activity of decoction  betel leaf with decoction basil leaf against growth of bacteria <em>Streptococcus mutans</em>.</p><p><strong> </strong></p><p><strong>Keywords</strong>: Betel leaf decoction, basil leaf  decoction, Streptococcus <strong>mutans.      </strong></p><p><strong> </strong></p><p><strong> </strong></p><p><strong>Abstrak</strong><strong></strong></p><p><strong> </strong></p><p><strong>Latar Belakang</strong>: Daun sirih mengandung minyak atsiri yang terdiri dari <em>bethelphenol, kavikol, </em>seskuiterpen, hydroxycavikol,cavibetol, estragol, eugenol dan carvacrol. Minyak atsiri bersifat antibakteri karena adanya senyawa phenol dan turunannya yang dapat mendenaturasi protein sel bakteri. Daun kemangi mengandung senyawa dari minyak atsiri yaitu <em>1,8-cineole</em>, <em>ß-bisabolene</em>, <em>metyl eugenol</em>. Ketiga bahan tersebut memiliki sifat larut terhadap etanol dan dapat menyebabkan kerusakan membran sel bakteri <em>streptococcus mutans</em> yang merupakan anggota flora normal rongga mulut tetapi dapat berubah menjadi patogen jika keseimbangan flora normal terganggu.Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antibakteri rebusan daun sirih (<em>piper betle</em> L) dengan rebusan daun kemangi (<em>ocimum sanctum</em>) terhadap pertumbuhan bakteri <em>Streptococcus mutans</em> (penelitian in vitro).</p><p><strong>Metode</strong>: penelitian observasional ini dengan teknik difusi. Penelitian ini dilakukan dengan mengamati dan mengukur diameter zona hambat pada MHA yang dibentuk oleh rebusan daun sirih (<em>piper betle</em> L) dan daun kemangi (<em>ocimum sanctum</em>) dalam satuan milimeter (mm). Terdapat 2 kelompok dengan replikasi sebanyak 16.</p><p><strong>Hasil</strong> : Hasil uji deskripsi menunjukkan bahwa aktivitas antibakteri pada rebusan daun sirih maupun rebusan daun kemangi yang tertinggi sebesar 17 mm dan yang terendah 15 mm. Tetapi pada nilai rata-rata efektifitas antibakteri rebusan daun sirih (15,81) lebih besar daripada nilai rata-rata efektifitas antibakteri rebusan daun kemangi (15,75). Hal ini dikarenakan ada zat kimia yang terkandung dalam daun sirih mirip dengan yang terkandung dalam daun kemangi, yaitu minyak atsiri.</p><p><strong>Kesimpulan</strong> : tidak ada perbedaan aktivitas antibakteri rebusan daun sirih dengan rebusan daun kemangi terhadap pertumbuhan bakteri <em>Streptococcus </em><em>m</em><em>utans</em>.</p><p><strong> </strong></p><p><strong>Kata kunci</strong>:  rebusan daun sirih, rebusan daun kemangi<em>, Streptococcus mutans</em>.</p><p> </p><p>     </p>

scholarly journals Inhibition of Heme Polymerization Invitro Assay Of Extract of Sirih Leaf (Piper betle Linn.) and Sun Flower Leaves (Helianthus annuus L.)

2020 ◽  
Vol 7 (1) ◽  
pp. 22 ◽  
Author(s):  
Ami Tjitraresmi ◽  
Moelyono Moektiwardoyo ◽  
Yasmiwar Susilawati
Keyword(s):  
Helianthus Annuus ◽  
Antimalarial Activity ◽  
Antimalarial Drugs ◽  
Piper Betle ◽  
Phytochemical Screening ◽  
Helianthus Annuus L ◽  
Betel Leaf ◽  
The People ◽  
Ic50 Values

Malaria is a disease that occurs in tropical countries like Indonesia. The incidence of malaria in the world is still quite high and the occurrence of cases of Plasmodium resistance to antimalarial drugs and the widespread of resistance have prompted researchers to look for new antimalarial drugs, especially from natural materials. Betel leaf (Piper betle Linn.) And sunflower leaf (Helianthus annuus L.) have long been used by the people of Indonesia as an antimalarial drug. The purpose of this study was to determine antimalarial activity through inhibition of heme polymerization and determine secondary metabolite compounds by phytochemical screening from betel leaves and sunflower leaves. The heme polymerization inhibition activity assay was carried out by the in-vitro method using a microplate reader at 415 nm and 630 nm wavelengths. IC50 values of betel leaf extract and sunflower leaf were 178.67 μg/ml and 160.10 μg/ml, respectively. Phytochemical screening results from betel leaf showed the presence of flavonoids, polyphenols, tannins, quinones, saponins, and monoterpenoids-sesquiterpenoids, while sunflower leaves contain alkaloids, polyphenols, flavonoids, steroids and monoterpenoids-sesquiterpenoids.Keywords: Piper betle Linn., Helianthus annuus L., Malaria, Heme Polymerization

scholarly journals BLUE LIGHT AND THE REGENERATION OF HUMAN RHODOPSIN IN SITU

1957 ◽  
Vol 41 (2) ◽  
pp. 419-428 ◽  
Author(s):  
W. A. H. Rushton
Keyword(s):  
Blue Light ◽  
Yellow Light ◽  
Human Eye ◽  
Equilibrium Level ◽  
Rapid Regeneration ◽  
Fundus Oculi

Hubbard has found that the photoisomerization of retinene was important for the regeneration of rhodopsin in vitro, and the object of the present investigation was to find whether this was also true for regeneration in the living human eye. In the Appendix is described a device which permits the rhodopsin density to be measured by analysing the light reflected from the fundus oculi in an ophthalmoscopic arrangement, the measurement taking about 5 seconds. Now if a blue and a yellow light viewed scotopically are adjusted in intensity so as to appear identical, they must bleach rhodopsin equally, but the blue will be more than 10 times as effective in isomerizing retinene. Therefore if retinene isomerization is important for rhodopsin regeneration, blue light should cause a more rapid regeneration after bleaching, and during bleaching the equilibrium level attained should be less profound. But, as the figures show, the course of bleaching and regeneration is identical for the matched yellow or blue bleaching lights, therefore isomerization of retinene is not important for rhodopsin regeneration in the living human eye.

Biochemical profiling of antifungal soap activity of betel leaf (Piper betle L.) extract and garlic oil by In vitro method

2020 ◽  
Vol 10 (4) ◽  
pp. 260-262
Author(s):  
Rutuja R Shah ◽  
Poonam J Patil ◽  
Pratibha R Adnaik ◽  
Rahul S Adnaik
Keyword(s):  
Piper Betle ◽  
In Vitro Method ◽  
Betel Leaf ◽  
Garlic Oil

In vitro mass propagation of Piper betle L

2019 ◽  
Vol 48 (3) ◽  
pp. 559-566
Author(s):  
Salim Khan ◽  
Barna Goswami ◽  
Shahina Akter ◽  
Mousona Islam ◽  
Afsana Huq Noon ◽  
...  
Keyword(s):  
Nodal Explants ◽  
Direct Regeneration ◽  
Leaf Segment ◽  
Piper Betle ◽  
Nodal Segment ◽  
Root Induction ◽  
Regeneration System ◽  
Petiole Explants ◽  
Best Response

An efficient in vitro regeneration system was developed for Piper betle L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best response on shoot/explants 13.2 ± 4.5 after 8 weeks of callus culture on MS medium with 0.5 mg/l BAP. The best response towards root induction was observed on half strength of MS with 0.25 mg/l IBA. The well rooted plants were successfully acclimatized and transferred to soil.

Evaluation of anti rheumatic activity of Piper betle L. (Betelvine) extract using in silico, in vitro and in vivo approaches

2020 ◽  
Vol 103 ◽  
pp. 104227
Author(s):  
Selvakumar Murugesan ◽  
Devibala Ravichandran ◽  
Dinesh Kumar Lakshmanan ◽  
Guna Ravichandran ◽  
Velusamy Arumugam ◽  
...  
Keyword(s):  
In Silico ◽  
Piper Betle
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