Phytochemical Screening, In-vitro Evaluation of Antioxidant and Free Radical Scavenging Activity of Leaves, Stems and Roots of Xanthium strumarium L., (Compositae)

2014 ◽  
Vol 4 (1) ◽  
pp. 1-22 ◽  
Author(s):  
Anjoo Kamboj
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Xanthium Strumarium ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Screening In Vitro

scholarly journals Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

2017 ◽  
Vol 9 (4) ◽  
pp. 615
Author(s):  
Mukesh Kumar Yadav ◽  
Santosh Kumar Singh ◽  
JS Tripathi ◽  
YB Tripathi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening

<p><em>Centella asiatica</em> also known as <em>mandukparni </em>or Indian pennywort or <em>jalbrahmi</em>, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. <em>Centella asiatica</em> has long been used to improve memory and cognitive function.</p><p>The study aimed to identify the phytochemicals present in different solvent extracts of <em>Centella asiatica </em>(i.e. PECA- Petroleum ether extract of <em>C. asiatica, </em>CCA- Chloroform extract of <em>C. asiatica, </em>EACA- Ethyl acetate extract of <em>C. asiatica,</em> ECA- Ethanolic extract of <em>C. asiatica, </em>HACA- Hydro-alcoholic extract of <em>C. asiatica</em>)<em> </em>and evaluate the respective in-vitro antioxidant potentials. <em></em></p><p>The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of <em>Centella asiatica</em> was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.</p><p>In all different solvent extracts of <em>C. asiatica</em> revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with  EC<sub>50</sub> values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC<sub>50</sub> values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity.</p><p> The present study indicates that the <em>Centella asiatica</em> extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.</p>

scholarly journals Phytochemical Screening, Free Radical Scavenging and α-Amylase Inhibitory Activities of Selected Medicinal Plants from Western Nepal

Medicines ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 70 ◽  
Author(s):  
Kusum Sai ◽  
Rashmi Thapa ◽  
Hari Prasad Devkota ◽  
Khem Raj Joshi
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Inhibitory Activities

Background: More than 700 plants are reported to be used for medicinal purposes in Nepal; however, many of them are not studied for their scientific evidences. The aims of the present study were the estimation of the total phenolic and flavonoid contents, and the evaluation of the free radical scavenging and α-amylase inhibitory activities of five selected medicinal plants from western Nepal: Aeschynanthus parviflorus Wall. (Gesneriaceae), Buddleja asiatica Lour. (Loganiaceae), Carica papaya L. (Caricaceae), Drepanostachyum falcatum (Nees) Keng f. (Gramineae) and Spondias pinnata (L. f.) Kurz (Anacardiaceae). Methods: The total phenolic content (TPC) and total flavonoid content (TFC) were measured using Folin-Ciocalteu’s phenol reagent and aluminium chloride methods, respectively. A 1,1–diphenyl–2–picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the free radical scavenging activity and an α-amylase inhibitory assay was carried out to determine the in vitro antidiabetic activity. Results: The phytochemical screening of five hydroalcoholic plant extracts revealed the presence of various secondary metabolites, including alkaloids, flavonoids, reducing sugars, saponins, terpenoids and tannins. The amounts of total phenolics and flavonoids were found to be the highest in B. asiatica leaf extract, which also showed the most potent free radical scavenging activity. Extract of C. papaya fruits showed the highest α-amylase inhibitory activity, whereas the extracts of B. asiatica leaves and S. pinnata leaves exhibited moderate activity. Conclusions: Some of the medicinal plants selected in this study showed high TPC and TFC values and potent bioactivities. These results may provide the scientific evidences of the traditional uses of these plants. However, further detailed studies on bioactive compounds isolation and identification and evaluation of in vivo pharmacological activities should be performed in future.

scholarly journals Antioxidant & analgesic activities of leaves of Panlata (Derris trifoliate): In vitro investigation

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Md Raihan Sarkar ◽  
Moynul Hasan ◽  
Md Sariful Islam Howlader ◽  
Mohammad Saydur Rahman ◽  
Shubhra Kanti Dey
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Immersion Method ◽  
Standard Drug ◽  
In Vitro Investigation

In the present study, the antioxidant and analgesic potential of the ethanolic extract of the leaves of Derris trifoliata was evaluated. The free radical scavenging activity of the crude extract on the stable radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) was determined by comparing the DPPH inhibitory capacity of the extract. In the quantitative assay, Derris trifoliata extract displayed a free radical scavenging activity in the DPPH assay (IC50 = 19 ?g/ml) which is comparable to that of ascorbic acid (IC50 = 7.80 ?g/ml), a well-known standard antioxidant. The analgesic responses of the given samples of extracts were evaluated using the Tail immersion method. In the analgesic activity test, extract at dose of 200 mg/kg and 400 mg/kg exhibited significant (P<0.05 and P<0.001 respectively) inhibition of pain by 166.82 and 184.95 after 120 and 180 minutes respectively while the standard drug Diclofenac Na inhibition was found to be 217.67 after 180 minutes at a dose of 25 mg/kg body weight DOI: http://dx.doi.org/10.3329/ijpls.v1i2.12951 International Journal of Pharmaceutical and Life Sciences Vol.1(2) 2012

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