scholarly journals Morphological Anomalies in Somatic Embryo Structure in Catharanthus roseus: Improving Embryo Germination by Amending Plant Growth Regulators, Activated Charcoal and Sucrose Level

2014 ◽  
Vol 4 (1) ◽  
pp. 10-20 ◽  
Keyword(s):  
Plant Growth ◽  
Somatic Embryo ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Catharanthus Roseus ◽  
Activated Charcoal ◽  
Embryo Germination ◽  
Morphological Anomalies ◽  
Sucrose Level

Effect of plant growth regulators on the biosynthesis of vinblastine, vindoline and catharanthine in Catharanthus roseus

2009 ◽  
Vol 60 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Qifang Pan ◽  
Yu Chen ◽  
Quan Wang ◽  
Fang Yuan ◽  
Shihai Xing ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Catharanthus Roseus

scholarly journals Enhanced Alkaloid Production from Cell Culture System of Catharanthus roseus in Combined Effect of Nutrient Salts, Sucrose and Plant Growth Regulators

2018 ◽  
Vol 1 (4) ◽  
pp. 14-34 ◽  
Author(s):  
Malay Ranjan Mishra ◽  
Rajesh Kumar Srivastava ◽  
Nasim Akhtar
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Suspension Culture ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Catharanthus Roseus ◽  
Culture System ◽  
Agar Medium ◽  
Alkaloid Content ◽  
Suspension Medium

Callus and biomass culture of Catharanthus roseus L. were established to check for the presence of total alkaloid and its subsequent yield. Various treatments like strength of nutrient salts, sucrose concentrations and combinations of plant growth regulators (PGR’s) were applied to both MS and B5 in agar as well as suspension medium to test their effects on enhanced alkaloid content and its yield. There was no significant difference in any of the observable parameters of fresh wt, dry wt, alkaloid content, production, productivity and yield if the culture were treated similarly in both types of media formulations (MS or B5 salts). Physical state (agar solidified or the liquid suspension) of the medium had significant effect on all the parameters particular on fresh wt, alkaloid content and yields. Although, the fresh wt. and dry wt. of biomass in suspension culture was 2-3 times less than that of callus obtained from agar medium. However, the alkaloid content and yield was 2-3 times higher in suspension culture compared to agar medium in similar treatments. The highest alkaloid content observed was 5.67mg/g dwt in B5 suspension medium containing 3% sucrose and modified with 0.5mg/l 2,4-Dichlorophenoxy acetic acid (2,4-D) + 1 mg/l Kinetin (KIN) + 2mg/l α- naphthalene acetic acid (NAA). The combined effects of these factors on the enhanced production of total alkaloids were expected to contain higher yield of anticancer vinblastine and vincristine in the cell suspension culture system.

scholarly journals Asymbiotic germination, seedling development and plantlet propagation of Encyclia aff. oncidioides - an endangered orchid

2011 ◽  
Vol 74 (3) ◽  
pp. 193-198 ◽  
Author(s):  
Ewa Łojkowska ◽  
Aleksandra Królicka ◽  
Matylda Sidwa-Gorycka ◽  
Jan J. Rybczyński ◽  
Dariusz L. Szlachetko ◽  
...  
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Beneficial Effect ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Activated Charcoal ◽  
Casein Hydrolysate ◽  
Seedling Development ◽  
Naphthaleneacetic Acid ◽  
Effect Of Light

In order to estimate the best germination conditions of <em>Encyclia</em> aff. <em>oncidioides</em> seeds, five different media (Fast, Knudson C modified by Vajrabhaya, Murashige and Skoog, PB2 and modified Vacin and Went) with different concentrations of plant growth regulators such as benzyladenine (BA), naphthaleneacetic acid (NAA) and gibberellic acid (GA3) were tested. No beneficial effect was observed when BA and NAA were applied to the germination medium and GA<sub>3</sub> inhibited germination. The effect of light, activated charcoal, coconut water and casein hydrolysate on seed germination was also studied. The growth rate of seedlings on three different media supplemented with activated charcoal and plant growth regulators was checked. The applied plant growth regulators had no beneficial effect on the further growth of seedlings. Fast and PB<sub>2</sub> media with 0.2% activated charcoal proved to be the best for <em>E.</em> aff. <em>oncidioides</em> seed germination, seedling development and plantlet propagation.

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