Tomato Inducer of CBF Expression 1 (SlICE1) is Involved in Cold and Salt Stress Signaling

Abstract Background Plasma membrane intrinsic proteins (PIPs) are plant channel proteins involved in water deficit and salinity tolerance. PIPs play a major role in plant cell water balance and responses to salt stress. Although sugarcane is prone to high salt stress, there is no report on PIPs in sugarcane. Results In the present study, eight PIP family genes, termed ScPIP1–1, ScPIP1–2, ScPIP1–3, ScPIP1–4, ScPIP2–1, ScPIP2–2, ScPIP2–4 and ScPIP2–5, were obtained based on the sugarcane transcriptome database. Then, ScPIP2–1 in sugarcane was cloned and characterized. Confocal microscopy observation indicated that ScPIP2–1 was located in the plasma membrane and cytoplasm. A yeast two-hybridization experiment revealed that ScPIP2–1 does not have transcriptional activity. Real time quantitative PCR (RT-qPCR) analysis showed that ScPIP2–1 was mainly expressed in the leaf, root and bud, and its expression levels in both below- and aboveground tissues of ROC22 were up-regulated by abscisic acid (ABA), polyethylene glycol (PEG) 6000 and sodium chloride (NaCl) stresses. The chlorophyll content and ion leakage measurement suggested that ScPIP2–1 played a significant role in salt stress resistance in Nicotiana benthamiana through the transient expression test. Overexpression of ScPIP2–1 in Arabidopsis thaliana proved that this gene enhanced the salt tolerance of transgenic plants at the phenotypic (healthier state, more stable relative water content and longer root length), physiologic (more stable ion leakage, lower malondialdehyde content, higher proline content and superoxide dismutase activity) and molecular levels (higher expression levels of AtKIN2, AtP5CS1, AtP5CS2, AtDREB2, AtRD29A, AtNHX1, AtSOS1 and AtHKT1 genes and a lower expression level of the AtTRX5 gene). Conclusions This study revealed that the ScPIP2–1-mediated osmotic stress signaling cascade played a positive role in plant response to salt stress.

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The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species

Plant and Cell Physiology ◽

10.1093/pcp/pcw006 ◽

2016 ◽

Vol 57 (3) ◽

pp. 528-539 ◽

Cited By ~ 22

Author(s):

Shilian Qi ◽

Qingfang Lin ◽

Huishan Zhu ◽

Fenghua Gao ◽

Wenhao Zhang ◽

...

Keyword(s):

Salt Stress ◽

Ring Finger ◽

E3 Ligase ◽

Stress Signaling ◽

Salt Tolerant ◽

Wild Tomato ◽

Wild Tomato Species ◽

Positive Regulator ◽

Tomato Species

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Salt-stress signaling

Journal of Plant Biology ◽

10.1007/bf03030623 ◽

2007 ◽

Vol 50 (2) ◽

pp. 148-155 ◽

Cited By ~ 33

Author(s):

Mi Sun Cheong ◽

Dae-Jin Yun

Keyword(s):

Salt Stress ◽

Stress Signaling

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Rice GTPase OsRacB: Potential Accessory Factor in Plant Salt-stress Signaling

Acta Biochimica et Biophysica Sinica ◽

10.1111/j.1745-7270.2006.00172.x ◽

2006 ◽

Vol 38 (6) ◽

pp. 393-402 ◽

Cited By ~ 7

Author(s):

Min LUO ◽

Su-Hai GU ◽

Shu-Hui ZHAO ◽

Fang ZHANG ◽

Nai-Hu WU

Keyword(s):

Salt Stress ◽

Stress Signaling ◽

Accessory Factor

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In silico identification of cis-Regulatory elements and their functional annotations from assembled ESTs of Artemisia annua L. involved in abiotic stress signaling

ROMANIAN BIOTECHNOLOGICAL LETTERS ◽

10.25083/rbl/26.2/2384.2395 ◽

2021 ◽

Vol 26 (2) ◽

pp. 2384-2395

Author(s):

PRAVEJ ALAM ◽

◽

THAMER AL BALAWI ◽

Keyword(s):

Salt Stress ◽

Abiotic Stress ◽

Artemisia Annua ◽

Regulatory Elements ◽

Common Side Effect ◽

Stress Signaling ◽

Functional Annotations ◽

In Silico Identification ◽

Plant Hormone Signaling ◽

Artemisinin Content

Salt stress is a common side-effect in plants impacted on plant growth, metabolism and productivity. A. annua L. is one of the well-known antimalarial plants, biosynthesized artemisinin in its leaf, now introduced in all-over the world. In this article, we have analyzed the A. annua L. ESTs under salt stress and predicted cis-regulatory elements, roles in abiotic stress signaling. Further, the predicted abiotic stress responsive factors were analyzed in order to their function annotations as compare to the genome of Arabidopsis thaliana. 11 EST-contigs assembled from 127 were 29 signals elements were identified by CAP3 program. In order to evaluate accuracy of the identified factors, gene ontology functions were performed. GOBP analysis enriched the genes (85.71%) as the response to abiotic signaling. The co-expression analysis was revealed by gene investigators and String 10.0, these factors-oriented genes had at least 0.40 correlations and 0.7 mutual connection. In projected PPI network, the recognized factors belong to plant hormone signaling and diterpene pathways. These factors (ABF1, APX CCC1, CPK6, JAZ1, MYC2) introduced as candidate genes responsive factors could be overexpressed in A. annua L. plants either alone or in a shuttle may led the good metabolism and higher artemisinin content.

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