scholarly journals High Voltage Electron Microscopic Image of Red Blood Cell in the Blood Vessel of Mouse Brain

2017 ◽  
Vol 47 (2) ◽  
pp. 75-76
Author(s):  
Im Joo Rhyu
Keyword(s):  
Blood Vessel ◽  
Blood Cell ◽  
Mouse Brain ◽  
High Voltage ◽  
Red Blood Cell ◽  
Microscopic Image ◽  
Electron Microscopic ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Electron Microscopic Image

High-voltage electron microscopic studies of inflammatory cell attachment during blood-brain barrier inflammation

1990 ◽  
Vol 48 (3) ◽  
pp. 276-277
Author(s):  
A.S. Lossinsky ◽  
M.J. Song
Keyword(s):  
High Voltage ◽  
Inflammatory Cell ◽  
Cell Attachment ◽  
Electron Microscopic ◽  
Vascular Perfusion ◽  
Tissue Samples ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
High Voltage Electron

Previous studies have suggested the usefulness of high-voltage electron microscopy (HVEM) for investigating blood-bram barrier (BBB) injury and the mechanism of inflammatory-cell (IC) attachment. These studies indicated that, in evaluating standard conventional thin sections, one might miss cellular attachment sites of ICs in their process of attaching to the luminal endothelial cell (EC) surface of cerebral blood vessels. Our current studies in animals subjected to autoimmune disease suggest that HVEM may be useful in localizing precise receptor sites involved in early IC attachment.Experimental autoimmune encephalomyelitis (EAE) was induced in mice and rats according to standard procedures. Tissue samples from cerebellum, thalamus or spinal cords were embedded in plastic following vascular perfusion with buffered aldehyde. Thick (0.5-0.7 μm) sections were cut on glass knives and collected on Formvar-coated slot grids stained with uranylacetate and lead citrate and examined with the AEI EM7 1.2 MV HVEM in Albany, NY at 1000 kV.

Three-Dimensional Analysis of Tranverse Tubules in Normal and Failing Heart: A Combined Confocal and High Voltage Electron Microscope Study

1997 ◽  
Vol 3 (S2) ◽  
pp. 231-232
Author(s):  
M. E. Martone ◽  
V. M. Edelman ◽  
A. Thor ◽  
S. J. Young ◽  
S. P. Lamont ◽  
...  
Keyword(s):  
High Voltage ◽  
Three Dimensional ◽  
Cardiac Cells ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Spontaneously Hypertensive ◽  
3 Dimensional ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
T Tubules

Early electron microscopic studies documented that significant changes in the membrane systems of cardiac cells occur in both ischemic and non-ischemic heart failure. These studies relied on analysis of two-dimensional sections and although quantitative changes were observed, the overall organization of the tranverse tubules (T-tubules) and the sarcoplasmic reticulum could not be assessed. In a 3-dimensional study using high voltage electron microscopy (EM) of the T-tubules in spontaneously hypertensive rats, Nakamura and Hama (1991) observed that concomitant with an increase in surface area, the T-tubule system becomes progressively more disorganized and exhibits structural irregularities such as increased numbers of longitudinal tubules, numerous short dead end branches and complex tubular aggregates. These authors suggested that this disorganization may interfere with synchronous contraction over the entire cell.In the present study, we examined the 3-dimensional organization of T-tubules in the left ventricle of explanted human hearts using confocal microscopy and EM tomography.

High-Voltage Electron Microscopic Study of Dislocations in Hydroxyapatite

1978 ◽  
Vol 57 (5-6) ◽  
pp. 708-708 ◽  
Author(s):  
T. Aoba ◽  
J. Takahashi ◽  
T. Yagi ◽  
M. Okazaki ◽  
Y. Moriwaki
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
High Voltage ◽  
Electron Microscopic ◽  
Voltage Electron ◽  
High Voltage Electron

High-voltage electron microscopic studies of the interrelationship between microglial cells and amyloid fibrils in scrapie-infected mouse brains

1991 ◽  
Vol 49 ◽  
pp. 86-87
Author(s):  
A. S. Lossinsky ◽  
M. J. Song ◽  
H. M. Wisniewski
Keyword(s):  
High Voltage ◽  
Cell Attachment ◽  
Microglial Cells ◽  
Stereo Pair ◽  
Amyloid Formation ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Amyloid Deposits

We have previously demonstrated the usefulness of high-voltage electron microscopy (HVEM) in the study of microvessels and inflammatory cell attachment in the central nervous system (CNS). In the present study, we used HVEM to further explore the interrelationship between microglial cells (MCs) and amyloid deposits in scrapie-infected mice. Scrapie infection in the mouse has been employed as an animal model to study the pathogenesis of amyloid fibril formation. The central question was whether three-dimensional (3-D) stereo-pair reconstruction would offer further insight into amyloid formation by MCs, which is currently the view of our group. Brains or cervical spinal cords from IM mice previously inoculated with 87V scrapie agent were used. One-half-micrometer thick plastic sections were stained with uranyl acetate and lead citrate. Light-microscopy views enabled us to target primary inoculation channels associated with amyloid deposits. Cells located at the periphery of the amyloid were identified as MCs (Fig. 1).

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