scholarly journals STABILITY INDICATING SPECTROPHOTOMETRIC METHODS OF NELFINAVIR AND THEIR APPLICATION TO IN-VITRO BIOEQUIVALENCE TESTING

2013 ◽  
Vol 2 (5) ◽  
pp. 29-33
Author(s):  
Fathi H Assaleh ◽  
Shanta Kumari Adiki ◽  
Shaban G Elosta ◽  
Fathi M Asseid ◽  
Prakash Katakam ◽  
...  
Keyword(s):  
Stability Indicating ◽  
Spectrophotometric Methods ◽  
Bioequivalence Testing

Acrylamide-induced oxidative stress in human erythrocytes

2009 ◽  
Vol 28 (10) ◽  
pp. 611-617 ◽  
Author(s):  
Betul Catalgol ◽  
Gül Özhan ◽  
Buket Alpertunga
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Human Erythrocytes ◽  
Antioxidant Enzyme Activities ◽  
Total Glutathione ◽  
Sod Activity ◽  
Spectrophotometric Methods ◽  
Low Concentrations ◽  
Different Doses

Acrylamide (AA), a widely used industrial chemical, is shown to be neurotoxic, mutagenic and carcinogenic. This study was carried out to investigate the effects of different doses of AA on lipid peroxidation (LPO), haemolysis, methaemoglobin (MetHb) and antioxidant system in human erythrocytes in vitro. Erythrocyte solutions were incubated with 0.10, 0.25, 0.50 and 1.00 mM of AA at 37°C for 1 hour. At the end of the incubation, malondialdehyde (MDA), an end product of LPO, was determined by liquid chromatography (LC) while total glutathione, reduced glutathione (GSH) levels, activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzymes and the rates of haemolysis and MetHb were determined by spectrophotometric methods. All of the studied concentrations of AA increased MetHb formation and SOD activity, and induced MDA formation and haemolysis due to the destruction of erythrocyte cell membrane. AA caused a decrease in the activities of GSH-Px, CAT and GSH levels. However, these effects of AA were seen only at higher concentrations than AA intake estimated for populations in many countries. We suggest that LPO process may not be involved in the toxic effects of AA in low concentrations, although the present results showed that the studied concentrations of AA exert deteriorating effects on antioxidant enzyme activities, LPO process and haemolysis.

In Vitro Bioequivalence Testing

2003 ◽  
pp. 449-455 ◽  
Author(s):  
Hansheng Wang ◽  
Ying Zhang ◽  
Jun Shao
Keyword(s):  
Bioequivalence Testing

Synthesis and DNA-binding Properties of a Cationic Seven-coordinate Manganese(II) Complex Formed with the Tripodal Ligand Tris(Nmethylbenzimidazol- 2-ylmethyl)amine and Salicylate

2012 ◽  
Vol 67 (8) ◽  
pp. 819-826 ◽  
Author(s):  
Huilu Wu ◽  
Ying Bai ◽  
Jingkun Yuan ◽  
Hua Wang ◽  
Guolong Pan ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Mode ◽  
X Ray Diffraction ◽  
Binding Properties ◽  
Tripodal Ligand ◽  
Spectrophotometric Methods ◽  
Physico Chemical ◽  
Dna Binding Properties ◽  
Hydroxyl Radical Scavenge

A ternary cationic Mn(II) complex with the tripodal ligand tris(2-(N-methyl) benzimidazylmethyl)amine (Mentb), salicylate and DMF as ligands and nitrate as counterion, [Mn(Mentb)(salicylate)DMF](NO3), was synthesized and characterized by physico-chemical and spectroscopic methods. The crystal structure of the Mn(II) complex has been determined by single-crystal X-ray diffraction and revealed that the central Mn(II) atom is seven-coordinated. The DNA-binding properties of the Mn(II) complex were investigated by spectrophotometric methods and viscosity measurements, and the results suggest that the Mn(II) complex binds to DNA via an intercalation binding mode. Additionally, the complex exhibited potential hydroxyl radical scavenge properties in in vitro studies

scholarly journals Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Rabie Kachkoul ◽  
Tarik Squalli Houssaini ◽  
Mohamed Mohim ◽  
Radouane El Habbani ◽  
Anissa Lahrichi
Keyword(s):  
Calcium Oxalate ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Stone Formation ◽  
Chemical Species ◽  
Punica Granatum ◽  
Fruit Peel ◽  
Spectrophotometric Methods ◽  
Dpph Method

The plant Punica granatum L. has several biological activities and a great curative and preventive power against chronic diseases. For this purpose, the objective of this work is to valorize the fruit peel of this plant in the field of phytomedicine, by quantifying and identifying its bioactive compounds and by evaluating their antioxidant and anticrystallization activities against calcium oxalate. This comparative study has been carried out by hydroalcoholic extract (E.PG) and infusion (I.PG) of the plant. The quantification of the phenolic compounds has been performed by spectrophotometric methods, and the chemical species identification has been performed by UPLC-PDA-ESI-MS. Moreover, the examination of the antioxidant activity has been executed by both methods of DPPH and FRAP. The crystallization inhibition has been studied in vitro by the turbidimetric model. The characterization of the synthesized crystals has been accomplished by microscopic observation and by Fourier Transform Infrared Spectroscopy. The results found show the comparable importance of the two plant extracts in the elimination of free radicals; the values of the half maximal inhibitory concentration “IC50” obtained are in the order of 60.87 ± 0.27 and 59.91 ± 0.83 μg/mL by the DPPH method and in the order of 42.17 ± 7.46 and 79.77 ± 6.91 μg/mL by the FRAP method, for both E.PG and I.PG, respectively. Furthermore, the inhibition percentages of calcium oxalate crystallization are in the range of 98.11 ± 0.17 and 98.22 ± 0.71% against the nucleation and in the order of 88.98 ± 0.98 and 88.78 ± 2.48% against the aggregation, for E.PG and I.PG, respectively. These results prove the richness of the plant in bioactive compounds, offering an antioxidant and anticrystallization capacity; therefore, it can be used in the treatment and/or the prevention of stone formation.

scholarly journals Detection of Genistein in Soy Protein Isolate and Soymilk Powder by Spectrophotometric and Chromatographic Method

2019 ◽  
Vol 11 ◽  
pp. 69-73
Author(s):  
Md. Anisur Rahman Mazumder ◽  
Parichat Hongsprabhas
Keyword(s):  
Soy Protein ◽  
Cultured Cells ◽  
Soy Protein Isolate ◽  
Milk Powder ◽  
Density Lipoprotein ◽  
Inhibitory Effect ◽  
Protein Isolate ◽  
Hplc Method ◽  
Spectrophotometric Methods

Genistein proposed as a treatment for osteoporosis for postmenopausal women, elderly men, lowering cardiovascular disease and reduces hormone dependent cancers. Genistein also exerted inhibitory effect on lipid peroxidation induced in vitro by pro-oxidant agents on model and natural membranes on cultured cells and on low density lipoprotein. Genistein detection in soy products is very much important for Food Scientist. Gensitein can be detected by UV-Visible spectrophotometric and HPLC method. This study focused on the detection of genistein by HPLC and spectrophotometric methods. Genistein content of both soy protein isolate (SPI) and spray dried soy milk powder (SMP) was determined by spectrophotometry (93.12±1.15 and 74.78±0.75 mg/100g, respectively) were slightly higher but not significantly differ than HPLC analysis (89.67±5.16 and 72.34±0.27 mg/100g, respectively). This study suggested that genistein and its glycoside could be detected by spectrophotometric methods with high accuracy.

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