scholarly journals Detection of Anaplasma phagocytophilum and Anaplasma bovis in Small Wild Mammals from Taichung and Kinmen Island, Taiwan

2014 ◽  
Vol 67 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Toshiyuki Masuzawa ◽  
Yoshiyuki Uchishima ◽  
Takashi Fukui ◽  
Yoshihiro Okamoto ◽  
Ming-Jeng Pan ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Wild Mammals ◽  
Anaplasma Bovis

scholarly journals Granulocytic anaplasmosis in captive ring-tailed lemur (Lemur catta) in Poland

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Łukasz Adaszek ◽  
Anna Wilczyńska ◽  
Jerzy Ziętek ◽  
Marcin Kalinowski ◽  
Oliwier Teodorowski ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Polymerase Chain Reaction Test ◽  
Anaplasma Ovis ◽  
Case Presentation ◽  
Granulocytic Anaplasmosis ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Obligate Intracellular Bacteria ◽  
Chain Reaction Test ◽  
Anaplasma Bovis

Abstract Background Anaplasma are obligate intracellular bacteria and aetiological agents of tick-borne diseases of both veterinary and medical interest. The genus Anaplasma comprises six species: Anaplasma marginale, Anaplasma centrale, Anaplasma ovis, Anaplasma phagocytophilum, Anaplasma bovis and Anaplasma platys. They can infect humans, carnivores, ruminants, rodents, insectivores, birds and reptiles. The aim of this study was to present the first clinical case of granulocytic anaplasmosis in a captive ring-tailed lemur in Poland. Case presentation A 4-year-old female lemur presented anorexia, epistaxis and tick infestation. The microscopic examination of a blood smear revealed morulae in neutrophils. Polymerase chain reaction test and sequencing of obtained PCR product confirmed infection by the GU183908 Anaplasma phagocytophilum strain. Therapeutic protocol included doxycycline (2.5 mg/kg p.o., b.i.d.) for 3 weeks and the lemur recovered within 24 h. Conclusions This is the first report on granulocytic anaplasmosis in a ring-tailed lemur in Europe, indicating that A. phagocytophilum infection must also be considered in differential diagnosis in this animal species, especially in individuals with thrombocytopenia associated with Ixodes ricinus parasitism.

Detection of Anaplasma bovis and Anaplasma phagocytophilum DNA from Haemaphysalis megaspinosa in Hokkaido, Japan

2010 ◽  
Vol 168 (1-2) ◽  
pp. 170-172 ◽  
Author(s):  
Kaoru Yoshimoto ◽  
Yuuki Matsuyama ◽  
Hironori Matsuda ◽  
Leo Sakamoto ◽  
Kotaro Matsumoto ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Anaplasma Bovis

Detection of Anaplasma bovis and Anaplasma phagocytophilum from cattle on Yonaguni Island, Okinawa, Japan

2008 ◽  
Vol 154 (3-4) ◽  
pp. 360-364 ◽  
Author(s):  
Mamoru Ooshiro ◽  
Satoshi Zakimi ◽  
Yoshimasa Matsukawa ◽  
Yoshito Katagiri ◽  
Hisashi Inokuma
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Anaplasma Bovis

scholarly journals Survey of Tick-borne-disease from Ornithodoros spp. in Uninhabited Islands of Korea.

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Sun-Woo Han ◽  
Yong-Sun Jo ◽  
Jeong Byoung Chae ◽  
Yoon Kyoung Cho ◽  
Nam-Shik Shin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Anaplasma Phagocytophilum ◽  
Economic Losses ◽  
Rrna Gene ◽  
Gene Fragment ◽  
Sequence Identification ◽  
Tick Borne Disease ◽  
Anaplasma Bovis ◽  
Breeding Place

ObjectiveThe aim of this study is to survey data of ticks distribution of Korean islands and to investigate pathogens in Argasid ticks.IntroductionTicks and tick-borne diseases have been thought global important issues, because it's affect to animal and human health and are the cause of significant economic losses. The genus Ornithodoros spp., which is included in Family Argasidae, is usually associated with wild animals including seabirds and it was difficult to investigate because seabirds’ nests are found in inaccessible uninhabited islands. However, Ornithodoros spp. has been known for the vector of many diseases including African swine fever.MethodsIn this study, nest with soil and litter of seabirds were collected, to investigate Ornithodoros species from 9 uninhabited islands, Nan-do, Chilsan-do, Chilbal-do, Sogukhol-do, Googul-do, Gaerin-do, Sasu-do, Hong-do (Hallyeohaesang) and Dok-do located western and southern part of the Korea from July, 2017 to September in 2018. The islands are known for breeding places of migratory and resident birds. Maximum ten nests with soil and litter of seabirds were collected from one uninhabited island for the conservation of the islands environment. Ticks were collected from nest with soil and litter of seabirds using Tullgren funnel and were assayed for tick identification by PCR using 16S rRNA gene and tick-borne pathogens including Rickettia spp., Borrelia spp., Bartonella spp., Ehrlichia chaffensis, Ehrlichia canis, Anaplasma phagocytophilum and Anaplasma bovis by nested PCR.ResultsTotal 65 Ornithodoros species ticks from 338 seabird's (black-tailed gull, Larus crassirotris; streaked shearwater, Calonectris leucomelas and Swinhoe's storm petrel, Oceanodroma monorhis) nesting soil with litter in 9 uninhabited islands. In the sequence identification of 16S rRNA gene fragment of Ornithodoros species, O. capensis and O. sawaii were 37 and 28, respectively. In the analyses of tick-borne pathogens, Borrelia spp. (n=5) was detected in O. sawaii from Gaerin-do and Googul-do. The total detection rate of Borrelia sp. from Ornithodoros spp. was 7.69% (5/65).ConclusionsIn this research, we discovered that O. sawaii are habitat in west and southern part of uninhabited islands to breeding place of black-tailed gulls and streaked shearwater and O. capensis are habitat in uninhabited islands related to breeding place of streaked shearwater. This is first report of the Borrelia spp. from Ornithodoros sawaii in Korea. 

scholarly journals Novel Genetic Variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a Novel Ehrlichia sp. in Wild Deer and Ticks on Two Major Islands in Japan

2006 ◽  
Vol 72 (2) ◽  
pp. 1102-1109 ◽  
Author(s):  
Makoto Kawahara ◽  
Yasuko Rikihisa ◽  
Quan Lin ◽  
Emiko Isogai ◽  
Kenji Tahara ◽  
...  
Keyword(s):  
United States ◽  
Anaplasma Phagocytophilum ◽  
The United States ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Blood Samples ◽  
Reservoir Hosts ◽  
Anaplasma Centrale ◽  
Wild Deer ◽  
Anaplasma Bovis

ABSTRACT Wild deer are one of the important natural reservoir hosts of several species of Ehrlichia and Anaplasma that cause human ehrlichiosis or anaplasmosis in the United States and Europe. The primary aim of the present study was to determine whether and what species of Ehrlichia and Anaplasma naturally infect deer in Japan. Blood samples obtained from wild deer on two major Japanese islands, Hokkaido and Honshu, were tested for the presence of Ehrlichia and Anaplasma by PCR assays and sequencing of the 16S rRNA genes, major outer membrane protein p44 genes, and groESL. DNA representing four species and two genera of Ehrlichia and Anaplasma was identified in 33 of 126 wild deer (26%). DNA sequence analysis revealed novel strains of Anaplasma phagocytophilum, a novel Ehrlichia sp., Anaplasma centrale, and Anaplasma bovis in the blood samples from deer. None of these have been found previously in deer. The new Ehrlichia sp., A. bovis, and A. centrale were also detected in Hemaphysalis longicornis ticks from Honshu Island. These results suggest that enzootic cycles of Ehrlichia and Anaplasma species distinct from those found in the United States or Europe have been established in wild deer and ticks in Japan.

scholarly journals Molecular survey of zoonotic Anaplasma phagocytophilum and genetic evidence of a putative novel Anaplasma species in goats from Taif, Saudi Arabia

2019 ◽  
pp. 54-59
Author(s):  
Mohamed W. Ghafar ◽  
Sayed A. M. Amer
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Public Health Importance ◽  
Specific Primers ◽  
Anaplasma Ovis ◽  
Variant Strain ◽  
Molecular Identity ◽  
Complete Identification ◽  
Polymerase Chain ◽  
Anaplasma Bovis ◽  
Positive Results

Aim: Genus Anaplasma is of veterinary and public health importance, and its members utilize ruminants as key hosts in their epidemiology. To date, information about the occurrence and molecular identity of Anaplasma phagocytophilum and other Anaplasma species in Saudi Arabian goats is scarce. This study aimed to molecularly detect and characterize zoonotic A. phagocytophilum and other Anaplasma spp. in goats from Taif District, KSA. Materials and Methods: Blood samples collected from 67 goats were polymerase chain reaction tested using common and A. phagocytophilum-specific primers targeting 16S rRNA and msp4 genes, respectively. Amplicons of common reactions were purified, sequenced, and analyzed. Results: Six goats yielded positive results with common primers, whereas all animals proved negative for A. phagocytophilum. Analysis of the two successfully sequenced amplicons revealed the presence of a variant strain of Anaplasma ovis (99.52% ID) and a new Anaplasma organism, which was clustered with Anaplasma bovis (95.9% ID) and Aegyptianella pullorum (94.99% ID) and distinctly separated from all other recognized species of the genus Anaplasma. Conclusion: The tested goats proved negative for A. phagocytophilum; however, we could not confirm that the area is pathogen free. A variant strain of A. ovis and a putative novel Anaplasma spp. were reported raising the concern of veterinary and zoonotic potential. Other genes should be sequenced and analyzed for complete identification of the detected organisms.

Sign in / Sign up

Export Citation Format

Share Document