scholarly journals Assessment of the Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Mycoplasma pneumoniae in Pediatric Community-Acquired Pneumonia

2013 ◽  
Vol 66 (6) ◽  
pp. 539-542 ◽  
Author(s):  
Kensei Gotoh ◽  
Naoko Nishimura ◽  
Suguru Takeuchi ◽  
Fumihiko Hattori ◽  
Kazuhiro Horiba ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Community Acquired Pneumonia ◽  
Rapid Diagnosis ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

scholarly journals Rapid detection of Mycoplasma pneumoniae by loop-mediated isothermal amplification assay

Medicine ◽  
2018 ◽  
Vol 97 (25) ◽  
pp. e10806 ◽  
Author(s):  
Xin Yuan ◽  
Changqing Bai ◽  
Qian Cui ◽  
Han Zhang ◽  
Jing Yuan ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

Rapid Diagnosis of Soya Bean Root Rot Caused byFusarium culmorumUsing a Loop-Mediated Isothermal Amplification Assay

2017 ◽  
Vol 165 (4) ◽  
pp. 249-256 ◽  
Author(s):  
Dandan Zeng ◽  
Wenwu Ye ◽  
Miao Xu ◽  
Chenchen Lu ◽  
Qing Tian ◽  
...  
Keyword(s):  
Root Rot ◽  
Rapid Diagnosis ◽  
Soya Bean ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Bean Root ◽  
Amplification Assay

scholarly journals Evaluation of Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Acanthamoeba Keratitis

2017 ◽  
Vol 35 (1) ◽  
pp. 90-94 ◽  
Author(s):  
Abhishek Mewara ◽  
Sumeeta Khurana ◽  
Shakila Yoonus ◽  
Kirti Megha ◽  
Parveen Tanwar ◽  
...  
Keyword(s):  
Acanthamoeba Keratitis ◽  
Rapid Diagnosis ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

Clinical utility of loop-mediated isothermal amplification for rapid diagnosis of Mycoplasma pneumoniae in children

2014 ◽  
Vol 63 (2) ◽  
pp. 248-251 ◽  
Author(s):  
Yuta Aizawa ◽  
Tomohiro Oishi ◽  
Shinya Tsukano ◽  
Tetsuo Taguchi ◽  
Akihiko Saitoh
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Lamp Assay ◽  
Cost Effective ◽  
Community Acquired Pneumonia ◽  
Isothermal Amplification ◽  
City Hospital ◽  
Serum Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Paired Serum ◽  
Median Interval

Loop-mediated isothermal amplification (LAMP) is a cost-effective and rapid method for identifying Mycoplasma pneumoniae (MP). We investigated the utility of the LAMP assay in diagnosing MP pneumonia among children in a clinical setting. In this prospective study, the cause of community-acquired pneumonia was evaluated in 111 patients for whom MP was the suspected pathogen. All participants were patients at a city hospital in Japan between April 2012 and September 2012. Throat swabs for the LAMP assay were obtained at admission, and paired serum samples to measure antibody titres to MP by particle agglutination were obtained at admission and during convalescence. Overall, 45 of 111 (41 %) patients had a fourfold or greater increase in MP titres and received a diagnosis of MP pneumonia. Among them, 43 (96 %) patients (median age, 9 years) were positive on the LAMP assay and had a fourfold or greater increase in MP titres. The median interval from fever onset to collection of throat swabs was 7 days (range, 4–10 days). As compared with paired serum titres, the LAMP assay enabled quicker diagnosis of MP (median interval, 13 vs. 7 days), thereby allowing early initiation of appropriate antimicrobial therapy.

Evaluation of a loop-mediated isothermal amplification assay for rapid diagnosis of soft-shelled turtle iridovirus

2011 ◽  
Vol 173 (2) ◽  
pp. 328-333 ◽  
Author(s):  
Zongxiao Liu ◽  
Hong Liu ◽  
Xiayang Xie ◽  
Junqiang He ◽  
Tingrong Luo ◽  
...  
Keyword(s):  
Rapid Diagnosis ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay
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