scholarly journals Isolation of an Intertypic Recombinant Human Adenovirus (Candidate Type 56) from the Pharyngeal Swab of a Patient with Pharyngoconjunctival Fever

2012 ◽  
Vol 65 (5) ◽  
pp. 457-459 ◽  
Author(s):  
Miki Enomoto ◽  
Teruo Okafuji ◽  
Takao Okafuji ◽  
Masatsugu Chikahira ◽  
Masami Konagaya ◽  
...  
Keyword(s):  
Human Adenovirus ◽  
Pharyngeal Swab ◽  
Intertypic Recombinant

scholarly journals An Outbreak of Acute Respiratory Infection at a Training Base in Beijing, China Due to Human Adenovirus Type B55

2020 ◽  
Author(s):  
Guilan Lu ◽  
Xiaomin Peng ◽  
Renqing Li ◽  
Yimeng Liu ◽  
Zhanguo Wu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Real Time ◽  
Respiratory Infection ◽  
Real Time Pcr ◽  
Acute Respiratory Infection ◽  
Index Case ◽  
Human Adenovirus ◽  
Whole Genome ◽  
Pharyngeal Swab ◽  
Tract Infections

Abstract Background: Twelve students experienced symptoms of acute respiratory infection (ARI) at a training base in Beijing from August 26 to August 30, 2015. We investigated the cause of this ARI outbreak. Methods: In partnership with the local center for disease control, we collected a total of twelve pharyngeal swab specimens as well as demographic information for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate HAdV, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber, and penton genes of the isolated adenoviruses. In addition, we analyzed the entire genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students using multiplex real-time PCR. None of the students were co-infected with other viruses. We successfully isolated seven HAdV strains from the pharyngeal swab specimens. The coding sequences of the hexon, fiber, and penton genes of these seven HAdV strains were identical, suggesting that they represented seven strains from a single virus clone. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. The genome of BJDX-01-2015 clustered with HAdV-B55 in phylogenetic analyses and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the strain associated with the August 2015 ARI outbreak at a training base in Beijing. This was the first reported outbreak in Beijing due to HAdV-B55. Continuous surveillance of respiratory adenoviruses is urgently needed to understand the epidemiological and evolutionary features of HAdV-B55, and an epidemiological modeling approach may provide further insights into this emerging public health threat. Furthermore, the clinical laboratory data from this outbreak provides important reference for the clinical diagnosis and may ultimately aid in informing the development of strategies to control and prevent respiratory tract infections caused by HAdV-B55. Keywords: Outbreak, Human adenovirus, Acute Respiratory Infection, Phylogenetic Analysis, Whole Genome Sequencing

scholarly journals Identification of a novel intertypic recombinant species D human adenovirus in a pediatric stem cell transplant recipient

2014 ◽  
Vol 61 (4) ◽  
pp. 496-502 ◽  
Author(s):  
Adriana E. Kajon ◽  
Daryl Lamson ◽  
Matthew Shudt ◽  
Zacharoula Oikonomopoulou ◽  
Brian Fisher ◽  
...  
Keyword(s):  
Stem Cell ◽  
Transplant Recipient ◽  
Stem Cell Transplant ◽  
Human Adenovirus ◽  
Cell Transplant ◽  
Intertypic Recombinant

scholarly journals An Outbreak of Acute Respiratory Illness at a Training Base in Beijing, China, Due to Human Adenovirus Type B55

2020 ◽  
Author(s):  
Guilan Lu ◽  
Xiaomin Peng ◽  
Renqing Li ◽  
Yimeng Liu ◽  
Zhanguo Wu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Index Case ◽  
Laboratory Data ◽  
Human Adenovirus ◽  
Respiratory Illness ◽  
Acute Respiratory Illness ◽  
Pharyngeal Swab ◽  
Evolutionary Features ◽  
Tract Infections

Abstract Background: Twelve students were diagnosed with symptoms of acute respiratory illness (ARI) at a training base in Beijing from August 26 to August 30, 2015. We investigated the cause of this ARI outbreak. Methods: In partnership with the local center for disease control, we collected a total of twelve pharyngeal swab specimens as well as demographic information for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate the causative agent, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber, and penton genes of the isolated adenoviruses. In addition, we analyzed the entire genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students using multiplex real-time PCR. None of the students were co-infected with other viruses. We successfully isolated seven HAdV strains from the pharyngeal swab specimens. The coding sequences of the hexon, fiber, and penton genes of these seven HAdV strains were identical, suggesting that they represented seven isolates of a single virus strain. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. The genome of BJDX-01-2015 clustered with HAdV-B55 in phylogenetic analyses and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the cause of the August 2015 ARI outbreak at this base. This outbreak was the first reported in Beijing due to HAdV-B55. Continuous surveillance of respiratory adenoviruses is urgently needed to understand the epidemiological and evolutionary features of HAdV-B55, and an epidemiological modeling approach may provide further insights into this emerging public health threat. Furthermore, the clinical laboratory data in this outbreak provided important reference for the clinical diagnosis and may ultimately aid to inform the development of strategies to control and prevent respiratory tract infections caused by HAdV-B55 later.

scholarly journals An Outbreak of Acute Respiratory Illness at a Training Base in Beijing, China, Due to Human Adenovirus Type B55

2019 ◽  
Author(s):  
Guilan Lu ◽  
Xiaomin Peng ◽  
Renqing Li ◽  
Yimeng Liu ◽  
Zhanguo Wu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Real Time ◽  
Real Time Pcr ◽  
Index Case ◽  
Human Adenovirus ◽  
Respiratory Illness ◽  
Whole Genome ◽  
Acute Respiratory Illness ◽  
Pharyngeal Swab ◽  
Evolutionary Features

Abstract Background: An outbreak of acute respiratory illness (ARI) occurred at a training base in Beijing. A total of 12 students were diagnosed with symptoms of ARI from August 26 to August 30, 2015. The cause of the ARI outbreak was investigated. Methods: In partnership with the local center for disease control, we conducted an epidemiological investigation on the outbreak, collecting a total of twelve pharyngeal swab specimens as well as patient demographic for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate the causative virus, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber and penton genes of the isolated adenoviruses. In addition, we analyzed the whole genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students by multiplex real-time PCR. None of the students were co-infected with other viruses. We successfully isolated seven strains from the pharyngeal swab specimens. Genetic analysis showed that the coding sequences of the hexon, fiber, and penton genes obtained from those seven HAdV strains were identical, suggesting that they represented seven isolates of a single virus strain. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. Phylogenetic analysis showed that the genome of BJDX-01-2015 clustered with HAdV-B55 and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the culprit of the ARI outbreak. This was the first reported outbreak in Beijing due to HAdV-B55 virus. Continuous surveillance of respiratory adenoviruses is urgently needed for understanding the epidemiological and evolutionary features of HAdV-B55 and could also find value in an epidemiological modeling approach.

scholarly journals An Outbreak of Acute Respiratory Infection at a Training Base in Beijing, China Due to Human Adenovirus Type B55

2020 ◽  
Author(s):  
Guilan Lu ◽  
Xiaomin Peng ◽  
Renqing Li ◽  
Yimeng Liu ◽  
Zhanguo Wu ◽  
...  
Keyword(s):  
Real Time ◽  
Respiratory Infection ◽  
Real Time Pcr ◽  
Acute Respiratory Infection ◽  
Index Case ◽  
Laboratory Data ◽  
Human Adenovirus ◽  
Pharyngeal Swab ◽  
Evolutionary Features ◽  
Tract Infections

Abstract Background: Twelve students experienced symptoms of acute respiratory infection (ARI) at a training base in Beijing from August 26 to August 30, 2015. We investigated the cause of this ARI outbreak. Methods: In partnership with the local center for disease control, we collected a total of twelve pharyngeal swab specimens as well as demographic information for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate HAdV, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber, and penton genes of the isolated adenoviruses. In addition, we analyzed the entire genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students using multiplex real-time PCR. None of the students were co-infected with other viruses. We successfully isolated seven HAdV strains from the pharyngeal swab specimens. The coding sequences of the hexon, fiber, and penton genes of these seven HAdV strains were identical, suggesting that they represented seven strains from a single virus clone. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. The genome of BJDX-01-2015 clustered with HAdV-B55 in phylogenetic analyses and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the strain associated with the August 2015 ARI outbreak at a training base in Beijing. This was the first reported outbreak in Beijing due to HAdV-B55. Continuous surveillance of respiratory adenoviruses is urgently needed to understand the epidemiological and evolutionary features of HAdV-B55, and an epidemiological modeling approach may provide further insights into this emerging public health threat. Furthermore, the clinical laboratory data from this outbreak provides important reference for the clinical diagnosis and may ultimately aid in informing the development of strategies to control and prevent respiratory tract infections caused by HAdV-B55.

scholarly journals An Outbreak of Acute Respiratory Infection at a Training Base in Beijing, China Due to Human Adenovirus Type B55

2020 ◽  
Author(s):  
Guilan Lu ◽  
Xiaomin Peng ◽  
Renqing Li ◽  
Yimeng Liu ◽  
Zhanguo Wu ◽  
...  
Keyword(s):  
Real Time ◽  
Respiratory Infection ◽  
Real Time Pcr ◽  
Acute Respiratory Infection ◽  
Index Case ◽  
Laboratory Data ◽  
Human Adenovirus ◽  
Pharyngeal Swab ◽  
Evolutionary Features ◽  
Tract Infections

Abstract Background: Twelve students experienced symptoms of acute respiratory infection (ARI) at a training base in Beijing from August 26 to August 30, 2015. We investigated the cause of this ARI outbreak. Methods: In partnership with the local center for disease control, we collected a total of twelve pharyngeal swab specimens as well as demographic information for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate HAdV, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber, and penton genes of the isolated adenoviruses. In addition, we analyzed the entire genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students using multiplex real-time PCR. None of the students were co-infected with other viruses. We successfully isolated seven HAdV strains from the pharyngeal swab specimens. The coding sequences of the hexon, fiber, and penton genes of these seven HAdV strains were identical, suggesting that they represented seven strains from a single virus clone. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. The genome of BJDX-01-2015 clustered with HAdV-B55 in phylogenetic analyses and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the strain associated with the August 2015 ARI outbreak at a training base in Beijing. This was the first reported outbreak in Beijing due to HAdV-B55. Continuous surveillance of respiratory adenoviruses is urgently needed to understand the epidemiological and evolutionary features of HAdV-B55, and an epidemiological modeling approach may provide further insights into this emerging public health threat. Furthermore, the clinical laboratory data from this outbreak provides important reference for the clinical diagnosis and may ultimately aid in informing the development of strategies to control and prevent respiratory tract infections caused by HAdV-B55.

Human Adenovirus Uptake by Preirnplantation Mouse Embryos

1972 ◽  
Vol 30 ◽  
pp. 268-269
Author(s):  
D. G. Chase ◽  
W. Winters ◽  
L. Piko
Keyword(s):  
Hela Cells ◽  
Human Adenovirus ◽  
Mouse Embryos ◽  
Equilibrium Density ◽  
Cell Stage ◽  
Virus Attachment ◽  
Preimplantation Mouse Embryos ◽  
Embryo Culture Medium ◽  
Preimplantation Mouse ◽  
Mouse Cells

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.

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