scholarly journals Development of Simple Diffusion Test Method by Using Solid Phase Micro Extraction Method and Its Application

2020 ◽  
Vol 23 (1) ◽  
pp. 1-9
Author(s):  
Toshiro MATSUMURA ◽  
Mika IKUTA ◽  
Yukitoki MORITA ◽  
Yoichi YAMASHITA ◽  
Tsutoshi IMANAKA ◽  
...  
Keyword(s):  
Extraction Method ◽  
Solid Phase ◽  
Test Method ◽  
Solid Phase Micro Extraction ◽  
Diffusion Test ◽  
Simple Diffusion

scholarly journals Analysis of Components in Crude Drugs by Headspace Solid Phase Micro Extraction Method. I

1996 ◽  
Vol 116 (3) ◽  
pp. 251-254
Author(s):  
Noriko YASUDA ◽  
Kazuo OTSUKI ◽  
Mayumi NISHIKAWA ◽  
Munehiro KATAGI ◽  
Hitoshi TSUCHIHASHI
Keyword(s):  
Extraction Method ◽  
Solid Phase ◽  
Solid Phase Micro Extraction ◽  
Crude Drugs

Time-Scale Shifting of Volatile Semiochemical Levels in Wild Type Lychnophora ericoides (Brazilian arnica) and Pollinator Records

Planta Medica ◽  
2020 ◽  
Author(s):  
Daniel Petinatti Pavarini ◽  
João Semir ◽  
João Luís Callegari Lopes ◽  
Ricardo Roberto da Silva ◽  
Norberto Peporine Lopes
Keyword(s):  
Extraction Method ◽  
Solid Phase ◽  
Leaf Age ◽  
Solid Phase Micro Extraction ◽  
Short Time Series ◽  
Chemical Profiling ◽  
In Situ Conditions ◽  
Short Time ◽  
First Time

Abstract Lychnophora ericoides is a Brazilian folk phytomedicine from Cerradoʼs “campus rupestris”. Its volatile organic compounds includes bisabolene-derivatives as major compounds. Herein we provide the chemical profiling of constitutive volatile sesquiterpenes from L. ericoides leaves, timeframe emissions surveys, and pollinators records. In situ samples of L. ericoides were harvested. A headspace-solid phase micro extraction method of pre-concentration was optimized. Identification was done through GC-MS. Isolation and structural elucidation were performed whenever necessary. Pollinators were registered in pictures and video. Short time-series and harmonic regressions determined rhythms of single compounds, and average chromatographic signal area was used to determine mono and sesquiterpene rhythms. Concluding, optimized headspace-solid phase micro extraction method of terpenes level analysis was reached. α-Pinene, β-pinene, α-terpinene, para-cymene, limonene, γ-terpinene, terpinen-4-ol, dehydro-sesquicineole, and β-guaiene were identified using GC-MS data. 11-dehydro cadinol and ortho-acetoxy bisabolol were elucidated. Sesquiterpenes concentrations were higher due to temperature rise, lower leaf age, and flowering seasons. Harmonic regressions determined that daylight might control levels of terpenes. Hummingbird, hemiptera insects, and wasps were recorded visiting Compositae capitulum for the first time. We studied nondomestic plants from in situ conditions and concluded that bisabolene-derivative levels were more abundant than monoterpenes during flowering throughout the summer.

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