Chromoblastomycosis Caused by Phialophora verrucosa in a Costa Rican Child with Skin Sequelae due to Snake Bite

Screening bioactive secondary metabolites from Costa Rican enviromental microbial communities

Planta Medica ◽

10.1055/s-0035-1556400 ◽

2015 ◽

Vol 81 (11) ◽

Author(s):

JJ Araya ◽

M Chavarría ◽

A Pinto-Tomás ◽

C Murillo ◽

L Uribe ◽

...

Keyword(s):

Secondary Metabolites ◽

Microbial Communities ◽

Costa Rican ◽

Bioactive Secondary Metabolites

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Molecular analysis of FVIII gene in severe HA patients of Costa Rica

Hämostaseologie ◽

10.1055/s-0037-1619099 ◽

2010 ◽

Vol 30 (S 01) ◽

pp. S150-S152

Author(s):

G. Jiménez-Cruz ◽

M. Mendez ◽

P. Chaverri ◽

P. Alvarado ◽

W. Schröder ◽

...

Keyword(s):

Factor Viii ◽

Coagulation Factor ◽

Costa Rican ◽

Factor Viii Gene ◽

Intron 22 Inversion ◽

Intron 1 ◽

Polymerase Chain ◽

Inversion Analysis

SummaryHaemophilia A (HA) is X-chromosome linked bleeding disorders caused by deficiency of the coagulation factor VIII (FVIII). It is caused by FVIII gene intron 22 inversion (Inv22) in approximately 45% and by intron 1 inversion (Inv1) in 5% of the patients. Both inversions occur as a result of intrachromosomal recombination between homologous regions, in intron 1 or 22 and their extragenic copy located telomeric to the FVIII gene. The aim of this study was to analyze the presence of these mutations in 25 HA Costa Rican families. Patients, methods: We studied 34 HA patients and 110 unrelated obligate members and possible carriers for the presence of Inv22or Inv1. Standard analyses of the factor VIII gene were used incl. Southern blot and long-range polymerase chain reaction for inversion analysis. Results: We found altered Inv22 restriction profiles in 21 patients and 37 carriers. It was found type 1 and type 2 of the inversion of Inv22. During the screening for Inv1 among the HA patient, who were Inv22 negative, we did not found this mutation. Discussion: Our data highlight the importance of the analysis of Inv22 for their association with development of inhibitors in the HA patients and we are continuous searching of Inv1 mutation. This knowledge represents a step for genetic counseling and prevention of the inhibitor development.

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Plasma Thrombin Assay using a Chromogenic Substrate in Disseminated Intravascular Coagulation due to Snake Bite Envenomation

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646807 ◽

1979 ◽

Vol 41 (03) ◽

pp. 544-552 ◽

Cited By ~ 3

Author(s):

R P Herrmann ◽

P E Bailey

Keyword(s):

Disseminated Intravascular Coagulation ◽

Degradation Products ◽

Snake Bite ◽

Chromogenic Substrate ◽

Coagulation Parameters ◽

Fibrinogen Degradation Products ◽

Intravascular Coagulation

SummaryUsing the chromogenic substrate, Tos-Gly-Pro-Arg-pNA-HCL (Chromozym TH, Boehringer Mannheim) plasma thrombin was estimated in six cases of envenomation by Australian elapid snakes. All patients manifested findings chracteristic of defibrination due to envenomation by these snakes. Fibrin-fibrinogen degradation products were grossly elevated, as was plasma thrombin in all cases.Following treatment with antivenene, all abnormal coagulation parameters returned rapidly towards normal by 24 hours and plasma thrombin disappeared.

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