scholarly journals The Potential of Glioblastoma Patient Symptoms to Diagnose and Predict Survival

Cureus ◽  
2021 ◽  
Author(s):  
Oliver D Mrowczynski ◽  
Ae L Yang ◽  
Jiangang Liao ◽  
Elias Rizk
Keyword(s):  
Glioblastoma Patient

Eight-year survival of a recurrent glioblastoma patient treated with molecularly tailored therapy: a case report

2018 ◽  
Vol 160 (12) ◽  
pp. 2387-2391 ◽  
Author(s):  
Quintino Giorgio D’Alessandris ◽  
Nicola Montano ◽  
Maurizio Martini ◽  
Tonia Cenci ◽  
Liverana Lauretti ◽  
...  
Keyword(s):  
Case Report ◽  
Recurrent Glioblastoma ◽  
Tailored Therapy ◽  
Glioblastoma Patient

scholarly journals Copper-Binding Small Molecule Induces Oxidative Stress and Cell-Cycle Arrest in Glioblastoma-Patient-Derived Cells

2018 ◽  
Vol 25 (5) ◽  
pp. 585-594.e7 ◽  
Author(s):  
Kenichi Shimada ◽  
Eduard Reznik ◽  
Michael E. Stokes ◽  
Lakshmi Krishnamoorthy ◽  
Pieter H. Bos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Small Molecule ◽  
Copper Binding ◽  
Glioblastoma Patient ◽  
Cycle Arrest

IMMU-51. SINGLE-CELL ANALYSIS OF TUMOR-ASSOCIATED MICROGLIA AND MACROPHAGES FROM HUMAN GLIOBLASTOMA

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi104-vi104
Author(s):  
Atul Anand ◽  
Rikke Sick Andersen ◽  
Mark Burton ◽  
Dylan Scott Lykke Harwood ◽  
Frantz Rom Poulsen ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Primary Brain Tumor ◽  
Tumor Type ◽  
Cell Analysis ◽  
Data Set ◽  
Human Glioblastoma ◽  
Glioblastoma Patient ◽  
Single Cell Rna Sequencing ◽  
Therapeutic Problem

Abstract Patients with glioblastoma, the most frequent and malignant primary brain tumor type, have a poor prognosis with a median survival of 14 months. A major therapeutic problem is chemoresistance. In surgically removed glioblastoma tissue, tumor-associated microglia and macrophages (TAMs) constitute up to 30 % of the total cells. TAMs are capable of secreting cytokines, chemokines and growth factors, thereby influencing the tumor microenvironment. However, the existence of different TAM subtypes and their role in glioblastoma is not fully comprehended and rarely considered therapeutically. This could explain why many glioblastoma clinical trials fail despite of promising preclinical results. This project aims to interrogate the existence and characteristics of different TAM subtypes in human glioblastoma biopsies in order to identify novel subpopulations and therapeutic targets. To study the heterogeneity in TAMs, CD11b+ cells were isolated from glioblastoma patient′s tissue, and single-cell RNA sequencing was performed using the 10X Genomics Chromium platform for single-cell generation and an Illumina NovaSeq6000 system for sequencing. We have sequenced TAMs from three glioblastomas and CD11b+ cells from brain tissue adjacent to two brain metastases samples. In the filtered data set of almost 71,000 CD11b+ cells, we were able to identify recently described TAM populations, such as an interferon-induced, a phagocytic, a hypoxic and a proliferating subset. Interestingly, we also discovered potential novel TAM subsets, such as a pro-angiogenic subset. We have detected a TAM population which is more complex than the established M1 and M2 phenotypes, constituting novel TAM subsets. We are currently investigating these findings to validate specific markers associated with these subpopulations, and for the identification of novel clinically relevant targets.

Targeted therapy with anlotinib for a leptomeningeal spread recurrent glioblastoma patient

2021 ◽  
pp. 407-414
Author(s):  
Cong Li ◽  
Wenyu Li ◽  
Shuang Dai ◽  
Aruna Sharma ◽  
Hari Shanker Sharma ◽  
...  
Keyword(s):  
Targeted Therapy ◽  
Recurrent Glioblastoma ◽  
Leptomeningeal Spread ◽  
Glioblastoma Patient

scholarly journals IMST-39. IDO1 IS PROGNOSTIC FOR GLIOBLASTOMA PATIENT SURVIVAL AND CENTRALLY CORRELATES WITH POTENTLY IMMUNOSUPPRESSIVE MEDIATORS

2016 ◽  
Vol 18 (suppl_6) ◽  
pp. vi95-vi95
Author(s):  
Lijie Zhai ◽  
Matthew Genet ◽  
Erik Ladomersky ◽  
Kristen Lauing ◽  
Meijing Wu ◽  
...  
Keyword(s):  
Patient Survival ◽  
Glioblastoma Patient

Glioblastoma Patient-Derived Cell Lines: Generation of Nonadherent Cellular Models from Brain Tumors

2020 ◽  
pp. 105-117
Author(s):  
Joris Guyon ◽  
Tiffanie Chouleur ◽  
Andreas Bikfalvi ◽  
Thomas Daubon
Keyword(s):  
Brain Tumors ◽  
Cell Lines ◽  
Cellular Models ◽  
Glioblastoma Patient

scholarly journals TMOD-01. CHARACTERIZATION OF PATIENT-DERIVED PRIMARY CELL LINES AND XENOGRAFTS FOR GLIOBLASTOMA

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi262-vi262 ◽  
Author(s):  
Noriyuki Kijima ◽  
Daisuke Kanematsu ◽  
Tomoko Shofuda ◽  
Masahiro Nonaka ◽  
Ryoichi Iwata ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Surface ◽  
Cell Lines ◽  
Primary Cell Culture ◽  
Surface Marker ◽  
Primary Cell ◽  
Cell Surface Marker ◽  
High Expression ◽  
Glioblastoma Patient ◽  
Primary Cell Lines

Abstract Patient-derived primary cell culture and xenograft are essential tools for translational research for glioblastoma. However, characteristics of each patient derived cell line and xenograft is not extensively studied. In this study, we aim to analyze the characteristics of our glioblastoma patient-derived cell lines and xenografts based on cell surface markers and their differentiation patterns. We have established 20 glioblastoma primary cell culture lines by serum free medium containing EGF and bFGF and found that primary cell culture lines could be classified based on the expression of CD133 and CD44. Four cell lines had high expression of both CD133 and CD44. Eleven cell lines had high expression of only CD44, three cell lines had high expression of only CD133, two cell lines had low expression of both CD133 and CD44. In addition when we induce differentiation, these cell lines showed differentiation to both glial and neuronal differentiation, but differentiation patterns were different depending on each cell line. Four cell lines showed predominant neuronal differentiation and others showed predominant glial differentiation. We next investigated in vivo characteristics of glioblastoma patient derived xenografts from these established cell lines. We have injected these cell lines into NOD/Shi-scid IL2Rγ KO mouse and histopathologically analyzed characteristics of xenografts. Each xenograft well recapitulated histological features of original patients’ tumors and tumor cells remarkably invade through subventricular zone. These results suggest that glioblastoma patient derived primary cell lines and xenografts have different characteristics of cell surface marker expressions and differentiation patterns, thus can classify these cell lines depending on cell surface marker expressions and differentiation patterns. Further analysis is needed to examine the biological importance of the differences in cell surface marker expressions and differentiation patterns.

scholarly journals High-Resolution Mutational Profiling Suggests the Genetic Validity of Glioblastoma Patient-Derived Pre-Clinical Models

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56185 ◽  
Author(s):  
Shawn E. Yost ◽  
Sandra Pastorino ◽  
Sophie Rozenzhak ◽  
Erin N. Smith ◽  
Ying S. Chao ◽  
...  
Keyword(s):  
High Resolution ◽  
Glioblastoma Patient ◽  
Mutational Profiling ◽  
Clinical Models
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