scholarly journals The Role of Computed Tomography and Real-Time Polymerase Chain Reaction in the Diagnosis and Prognostication of COVID-19

Cureus ◽  
2021 ◽  
Author(s):  
James T Roberts ◽  
Jaron M Hrushka ◽  
Samuel O Krider ◽  
Quan D Nguyen
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

Periodontitis Salivary Microbiota Worsens Colitis

2021 ◽  
pp. 002203452110497
Author(s):  
J. Qian ◽  
J. Lu ◽  
Y. Huang ◽  
M. Wang ◽  
B. Chen ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bowel Disease ◽  
Chain Reaction ◽  
Salivary Microbiota ◽  
Inflammatory Bowel ◽  
Polymerase Chain

Evidence suggests that periodontitis contributes to the pathogenesis of inflammatory bowel disease, including Crohn’s disease and ulcerative colitis. However, few studies have examined the role of swallowing and saliva in the pathogenesis of gastrointestinal diseases. Saliva contains an enormous number of oral bacteria and is swallowed directly into the intestine. Here, we explored the influence of periodontitis salivary microbiota on colonic inflammation and possible mechanisms in dextran sulfate sodium (DSS)–induced colitis. The salivary microbiota was collected from healthy individuals and those with periodontitis and gavaged to C57BL/6 mice. Periodontitis colitis was induced by DSS for 5 d and ligature for 1 wk. The degree of colon inflammation was evaluated through hematoxylin and eosin staining, ELISA, and quantitative real-time polymerase chain reaction. Immune parameters were measured with quantitative real-time polymerase chain reaction, flow cytometry, and immunofluorescence. The gut microbiota and metabolome analyses were performed via 16S rRNA gene sequencing and liquid chromatography–mass spectrometry. Although no significant colitis-associated phenotypic changes were found under physiologic conditions, periodontitis salivary microbiota exacerbated colitis in a periodontitis colitis model after DSS induction. The immune response more closely resembled the pathology of ulcerative colitis, including aggravated macrophage M2 polarization and Th2 cell induction (T helper 2). Inflammatory bowel disease–associated microbiota, such as Blautia, Helicobacter, and Ruminococcus, were changed in DSS-induced colitis after periodontitis salivary microbiota gavage. Periodontitis salivary microbiota decreased unsaturated fatty acid levels and increased arachidonic acid metabolism in DSS-induced colitis, which was positively correlated with Aerococcus and Ruminococcus, suggesting the key role of these metabolic events and microbes in the exacerbating effect of periodontitis salivary microbiota on experimental colitis. Our study demonstrated that periodontitis contributes to the pathogenesis of colitis through the swallowing of salivary microbiota, confirming the role of periodontitis in systemic disease and providing new insights into the etiology of gastrointestinal inflammatory diseases.

scholarly journals Transferring of Lactobacillus antibiotic resistant genes to Salmonella

2021 ◽  
pp. 145-151
Author(s):  
Muhammad Ashraf ◽  
Sajjad-ur- Rahman ◽  
Muhammad Jawad Bashir ◽  
Rizwan Aslam ◽  
Sultan Ali ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Antibiotic Resistance ◽  
Real Time ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Growth Promoters ◽  
Chain Reaction ◽  
Antibiotic Resistant ◽  
Polymerase Chain

Antibiotic resistance is a worldwide issue and becoming more problematic due to extensive misuse of antibiotics. The present study was aimed to analyze role of Lactobacillus in transmission of antibiotic resistance genes (tetM, ermB, sul2) to Salmonella and verification of these genes by real time polymerase chain reaction. A total of thirty fecal samples (15 were indigenous and 15 were broilers) were collected and analyzed by real time polymerase chain reaction. The results indicated that there was high expression of antibiotic resistance genes in Lactobacillus in case of broiler chicken than indigenous ones indicating Lactobacillus as a reservoir of antibiotic resistance genes but found to be non-significant in transferring these genes to Salmonella. In conclusion, the excessive use of animal growth promoters in poultry assists in acquisition of antibiotic resistance genes by normal micro-biota. Keywords: Broiler, Non-significant, Antibiotic resistance, Real time polymerase chain

scholarly journals Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138249 ◽  
Author(s):  
Aquino Albino Nhantumbo ◽  
Vlademir Vicente Cantarelli ◽  
Juliana Caireão ◽  
Alcides Moniz Munguambe ◽  
Charlotte Elizabeth Comé ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Meningitis ◽  
Real Time ◽  
Burden Of Disease ◽  
Critical Role ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Role of Chest Computed Tomography versus Real Time Reverse Transcription Polymerase Chain Reaction for Diagnosis of COVID-19: A Systematic Review and Meta-Analysis

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Dina M. Ali ◽  
Lamiaa G. Zake ◽  
Nevine K. El Kady
Keyword(s):  
Computed Tomography ◽  
Systematic Review ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Meta Analysis ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Chest Computed Tomography ◽  
Polymerase Chain

Background. The current global pandemic of COVID-19 is considered a public health emergency. The diagnosis of COVID-19 depends on detection of the viral nucleic acid by real time reverse transcription polymerase chain reaction (RT-PCR). However, false-negative RT-PCR tests are reported and could hinder the control of the pandemic. Chest computed tomography could achieve a more reliable diagnosis and represent a complementary diagnostic tool. Aim. To perform a meta-analysis and systematic review to find out the role of chest computed tomography versus RT-PCR for precise diagnosis of COVID-19 infection. Methods. We searched three electronic databases (PubMed, ScienceDirect, and Scopus) from April 1 to April 20, 2020, to find out articles including the accuracy of chest computed tomography scan versus RT-PCR for diagnosis of SARS-CoV-2 infection. Observational studies, case series, and case reports were included. Results. A total of 238 articles were retrieved from the search strategy. Following screening, 39 articles were chosen for full text assessment and finally 35 articles were included for qualitative and quantitative analysis. Chest computed tomography showed a wide range of sensitivity varied from 12%–100%. Conclusion. Chest computed tomography is playing a key role for diagnosis and detection of COVID-19 infection. Computed tomography image findings may precede the initially positive RT-PCR assay.

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