Urinary Neutrophil Gelatinase-Associated Lipocalin Does Not Distinguish Acute Rejection from Other Causes of Acute Kidney Injury in Pediatric Renal Transplant Recipients

2017 ◽  
Vol 63 (01/2017) ◽  
Author(s):  
Tomas Seeman ◽  
Karel Vondrak ◽  
Jiri Dusek ◽  
Nadezda Simankova ◽  
Jakub Zieg ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Renal Transplant ◽  
Acute Rejection ◽  
Kidney Injury ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Pediatric Renal Transplant ◽  
Pediatric Renal Transplant Recipients ◽  
Neutrophil Gelatinase

scholarly journals Comparison of the Emit Immunoassay with HPLC for Therapeutic Drug Monitoring of Mycophenolic Acid in Pediatric Renal-Transplant Recipients on Mycophenolate Mofetil Therapy

2002 ◽  
Vol 48 (3) ◽  
pp. 517-525 ◽  
Author(s):  
Lutz T Weber ◽  
Maria Shipkova ◽  
Victor W Armstrong ◽  
Natalie Wagner ◽  
Ekkehard Schütz ◽  
...  
Keyword(s):  
Mycophenolate Mofetil ◽  
Renal Transplant ◽  
Acute Rejection ◽  
Mycophenolic Acid ◽  
Therapeutic Drug ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Increased Risk ◽  
Pediatric Renal Transplant ◽  
Pediatric Renal Transplant Recipients

Abstract Background: HPLC is currently the preferred method for accurate measurement of mycophenolic acid (MPA). This study was designed to validate the Emit compared with HPLC in relation to clinical outcome measurements. Methods: Pediatric renal-transplant recipients (n = 50) on an immunosuppressive triple regimen consisting of cyclosporin A, prednisone, and mycophenolate mofetil (600 mg/m2 twice per day) were investigated in an open-label prospective study. Pharmacokinetic profiles over 12 h were obtained at 1 week, 3 weeks, 3 months, and 6 months posttransplant. Plasma MPA was measured by both reversed-phase HPLC and the Emit immunoassay. Results: There was an association between the risk of acute rejection episodes and low area under the curve values from t0 to t12h (AUC0–12) for MPA (MPA-AUC0–12) or predose concentrations of MPA derived from both HPLC and Emit measurements. According to ROC analysis, an AUC value of 33.8 mg · h/L for MPA from t0 to t12h (MPA-AUC0–12) determined by HPLC had a diagnostic sensitivity of 80% and a diagnostic specificity of 57%. The corresponding value of the Emit was 36.1 mg · h/L. For the predose concentration (MPA-c12), a concentration of 1.2 mg/L determined by HPLC and 1.4 mg/L determined by Emit gave a sensitivity of 80% and a specificity of 60%, respectively. There was no association of any pharmacokinetic variables derived from total MPA measurements with an increased risk of side effects related to mycophenolate mofetil. Conclusions: The Emit assay appears to have a comparable diagnostic efficacy to HPLC for assessing the risk of acute rejection in pediatric renal-transplant recipients. However, because of the cross-reactivity of the antibody used in the Emit assay with the active MPA acyl glucuronide metabolite, the decision thresholds for the Emit were higher than those calculated from HPLC measurements.

SP873EFFECTS OF LATE ACUTE REJECTION ON GRAFT SURVIVAL AND OUTCOME IN PEDIATRIC RENAL TRANSPLANT RECIPIENTS

2015 ◽  
Vol 30 (suppl_3) ◽  
pp. iii665-iii665 ◽  
Author(s):  
Esra Baskin ◽  
Asli Kantar ◽  
Kaan Gulleroglu ◽  
Esra Ozmen ◽  
Handan Ozdemir ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Acute Rejection ◽  
Graft Survival ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Pediatric Renal Transplant ◽  
Pediatric Renal Transplant Recipients

Cyclosporin A Absorption Profiles in Pediatric Renal Transplant Recipients Predict the Risk of Acute Rejection

2004 ◽  
Vol 26 (4) ◽  
pp. 415-424 ◽  
Author(s):  
Lutz T. Weber ◽  
Victor W. Armstrong ◽  
Maria Shipkova ◽  
Reinhard Feneberg ◽  
Manfred Wiesel ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Acute Rejection ◽  
Cyclosporin A ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Pediatric Renal Transplant ◽  
Pediatric Renal Transplant Recipients
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