The application of differential pulse cathodic stripping voltammetry in the determination of trace copper in whole blood

2013 ◽  
Author(s):  
Tarik Attar ◽  
Nouria Dennouni-Medjati ◽  
Yahia Harek ◽  
Lahcène Larabi
Keyword(s):  
Whole Blood ◽  
Stripping Voltammetry ◽  
Differential Pulse ◽  
Cathodic Stripping Voltammetry ◽  
Cathodic Stripping

scholarly journals Determination of copper in whole blood by differential pulse adsorptive stripping voltammetry

2014 ◽  
Vol 2 (6) ◽  
pp. 691-700 ◽  
Author(s):  
Tarik Attar ◽  
Yahia Harek ◽  
Lahcene Larabi
Keyword(s):  
Whole Blood ◽  
Supporting Electrolyte ◽  
Stripping Voltammetry ◽  
Differential Pulse ◽  
Accumulation Time ◽  
Cathodic Stripping Voltammetry ◽  
Accumulation Potential ◽  
Determination Of Copper ◽  
Cathodic Stripping

 A selective and sensitive method for determination of copper in blood by adsorptive differential pulse cathodic stripping voltammetry is presented. The method is based on adsorptive accumulation of the complexes of Cu (II) ions with benzenesulfonyl hydrazide onto hanging mercury drop electrode (HMDE), followed by the reduction of the adsorbed species by differential pulse cathodic stripping voltammetry. The effect of various parameters such as supporting electrolyte, concentration of benzenesulfonyl hydrazide, accumulation potential, accumulation time and stirring rate on the selectivity and sensitivity were studied. The optimum conditions for determination of copper include perchloric acid 0.03 M, concentration of benzenesulfonyl hydrazide 7.5×10-5 M, the accumulation potential of -350 mV (vs. Ag/AgCl), the accumulation time of 50 s, and the scan rate of 50 mV s-1. Under optimized conditions, linear calibration curves were established for the concentration of Cu (II) in the range of 0.62-275 ng mL-1, with detection limit of 0.186 ng mL-1 for Cu (II). The procedure was successfully applied to the determination of copper ion in whole blood samples.

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