scholarly journals Biological function of Klebsiella variicola and its effect on the rhizosphere soil of maize seedlings

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9894
Author(s):  
Lijuan Yang ◽  
Kejun Yang
Keyword(s):  
Biological Function ◽  
Rhizosphere Soil ◽  
Potassium Dichromate ◽  
Colorimetric Method ◽  
Soil Salinization ◽  
Available Phosphorus ◽  
Plant Growth Promoting Bacteria ◽  
Alkali Stress ◽  
Maize Seedlings ◽  
Plant Growth Promoting

Background Deterioration of the ecological environment in recent years has led to increasing soil salinization, which severely affects the cultivation of agricultural crops. While research has focused on improving soil environment through the application of pollution-free microbial fertilizers, there are relatively few plant growth-promoting bacteria suitable for saline-alkali environments. Although Klebsiella variicola can adapt to saline-alkali environments to successfully colonize rhizosphere microenvironments, only a few studies have investigated its role in promoting crop growth. Its effect on the crop rhizosphere soil microenvironment is especially unclear. Methods In this study, the biological function of K. variicola and its colonization in maize seedling rhizosphere soil were studied in detail through selective media and ultraviolet spectrophotometry. The effects of K. variicola on the rhizosphere soil microenvironment and the growth of maize seedlings in saline-alkali and neutral soils were systematically analysed using the colorimetric method, the potassium dichromate volumetric method, and the diffusion absorption method. Results Our results showed that K. variicola played a role in indole acetic acid, acetoin, ammonia, phosphorus, and potassium production, as well as in nitrogen fixation. A high level of colonization was observed in the rhizosphere soil of maize seedlings. Following the application of K. variicola in neutral and saline-alkali soils, the nutrient composition of rhizosphere soil of maize seedlings increased in varying degrees, more notably in saline-alkali soil. The content of organic matter, alkali-hydrolysable nitrogen, available phosphorus, available potassium, alkaline phosphatase, sucrase, urease, and catalase increased by 64.22%, 117.39%, 175.64%, 28.63%, 146.08%, 76.77%, 86.60%, and 45.29%, respectively, insaline-alkalisoil. Conclusion K.variicola, therefore, performed a variety of biological functions to promote the growth of maize seedlings and effectively improve the level of soil nutrients and enzymes in the rhizosphere of maize seedlings, undersaline-alkali stress conditions. It played an important role in enhancing the rhizosphere microenvironment of maize seedlings under saline-alkali stress.

scholarly journals Metabolomic Analysis of Rhizosphere Soil Fertility in Maize (Zea mays) at Milking Stage

2021 ◽  
Vol 25 (03) ◽  
pp. 555-566
Author(s):  
Yunpeng Luan
Keyword(s):  
Total Nitrogen ◽  
Rhizosphere Soil ◽  
Potassium Dichromate ◽  
Colorimetric Method ◽  
Acid Soils ◽  
Ammonium Nitrogen ◽  
Available Phosphorus ◽  
Kjeldahl Method ◽  
Phosphorus And Potassium ◽  
Tof Ms

In this study rhizosphere soil of cornat a milk stage was collected to investigate characteristic metabolites and their potential functions. Total nitrogen, organic matters, ammonium nitrogen, pH values, available phosphorus and potassium were determined by semimicro-Kjeldahl method, potassium dichromate (external heating) method, indophenol blue colorimetric method, potentiometry, NaHCO3leaching-molybdenum-antimony colorimetric method and NH4OAc leaching-flame spectrometry, respectively. In addition, UPLC-Q/TOF-MS was adopted for non-targeted metabolomicanalysis. As revealed by results, the total nitrogen contents in soils collected from Dongchuang (i.e., DCMRS for short)0.67±0.14mg/kg lower than from Fumin (i.e., FMMRS for short); moreover, both DCMRS and FMMRS were acid soils. DCMRS contains higher levels of AN (Ammonium nitrogen), SOC (Soil organic carbon), and AP (Available phosphorus) than FMMRS. The amount of TN (Total nitrogen) contained in FMMRS soil was2.410±0.422mg/kg, which is higher than DCMRS. All data derived from UPLC-Q/TOF-MS met the corresponding requirements for further analysis. Metabolites such as 2-methyl-1-propylamine, gamma-butyrolactone and 3-methyl-1-butylamine were detected in DCMRS and FMMRS samples. Several pathways were included, such as lipid metabolism, xenobioticsbiodegradation and metabolism, terpenoids and polyketides, and amino acid metabolism. Through comparison of FMMRS and DCMRS, metabolic pathways associated with nitrogen, carbon, and antibiotic metabolism including iron transport were significantly different between them. Taken together, FMMRS is more fertile, less acidic, and higher in nitrogen than DCMRS.© 2021 Friends Science Publishers

scholarly journals Plant-Growth-Promoting Bacteria Mitigating Soil Salinity Stress in Plants

2020 ◽  
Vol 10 (20) ◽  
pp. 7326
Author(s):  
Stefan Shilev
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Soil Salinity ◽  
Soil Salinization ◽  
Plant Growth Promoting Bacteria ◽  
Plant Tolerance ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil deterioration has led to problems with the nutrition of the world’s population. As one of the most serious stressors, soil salinization has a negative effect on the quantity and quality of agricultural production, drawing attention to the need for environmentally friendly technologies to overcome the adverse effects. The use of plant-growth-promoting bacteria (PGPB) can be a key factor in reducing salinity stress in plants as they are already introduced in practice. Plants having halotolerant PGPB in their root surroundings improve in diverse morphological, physiological, and biochemical aspects due to their multiple plant-growth-promoting traits. These beneficial effects are related to the excretion of bacterial phytohormones and modulation of their expression, improvement of the availability of soil nutrients, and the release of organic compounds that modify plant rhizosphere and function as signaling molecules, thus contributing to the plant’s salinity tolerance. This review aims to elucidate mechanisms by which PGPB are able to increase plant tolerance under soil salinity.

scholarly journals Dynamics ofPanax ginsengRhizospheric Soil Microbial Community and Their Metabolic Function

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Yong Li ◽  
YiXin Ying ◽  
WanLong Ding
Keyword(s):  
Bacterial Communities ◽  
Rhizosphere Soil ◽  
Restriction Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Plant Growth Promoting Bacteria ◽  
Metabolic Function ◽  
Rhizosphere Soils ◽  
Soil Microbial ◽  
Culture Independent ◽  
Plant Growth Promoting

The bacterial communities of 1- to 6-year ginseng rhizosphere soils were characterized by culture-independent approaches, random amplified polymorphic DNA (RAPD), and amplified ribosomal DNA restriction analysis (ARDRA). Culture-dependent method (Biolog) was used to investigate the metabolic function variance of microbe living in rhizosphere soil. Results showed that significant genetic and metabolic function variance were detected among soils, and, with the increasing of cultivating years, genetic diversity of bacterial communities in ginseng rhizosphere soil tended to be decreased. Also we found thatVerrucomicrobia,Acidobacteria, andProteobacteriawere the dominants in rhizosphere soils, but, with the increasing of cultivating years, plant disease prevention or plant growth promoting bacteria, such asPseudomonas,Burkholderia, andBacillus, tended to be rare.

scholarly journals A Multifactorial Approach to Untangle Graphene Oxide (GO) Nanosheets Effects on Plants: Plant Growth-Promoting Bacteria Inoculation, Bacterial Survival, and Drought

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 771
Author(s):  
Tiago Lopes ◽  
Catarina Cruz ◽  
Paulo Cardoso ◽  
Ricardo Pinto ◽  
Paula A. A. P. Marques ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plant Growth ◽  
Food Production ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Survival ◽  
Maize Seedlings ◽  
Combined Application ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rhizobium Sp

Drought is a limiting factor for agricultural productivity. Climate change threatens to expand the areas of the globe subjected to drought, as well as to increase the severity and duration of water shortage. Plant growth-promoting bacteria (PGPB) are widely studied and applied as biostimulants to increase plant production and to enhance tolerance to abiotic and biotic constraints. Besides PGPB, studies on the potential of nanoparticles to be used as biostimulants are also thriving. However, many studies report toxicity of tested nanoparticles in bacteria and plants in laboratory conditions, but few studies have reported effects of nanoparticles towards bacterial cells and communities in the soil. The combined application of nanoparticles and PGPB as biostimulant formulations are poorly explored and it is important to unravel the potentialities of their combined application as a way to potentiate food production. In this study, Rhizobium sp. E20-8 and graphene oxide (GO) nanosheets were applied on container-grown maize seedlings in watered and drought conditions. Bacterial survival, seedling growth (dry weight), and biochemical endpoints (photosynthetic pigments, soluble and insoluble carbohydrates, proline, lipid peroxidation, protein, electron transport system, and superoxide dismutase) were evaluated. Results showed that the simultaneous exposure to GO and Rhizobium sp. E20-8 was able to alleviate the stress induced by drought on maize seedlings through osmotic and antioxidant protection by GO and mitigation of GO effects on the plant’s biochemistry by Rhizobium sp. E20-8. These results constitute a new lead on the development of biostimulant formulations to improve plant performance and increase food production in water-limited conditions.

Phosphate Solubilization and Plant Growth Promoting Actinobacteria from Rhizosphere Soil

2020 ◽  
Author(s):  
K. Balakrishnan ◽  
J. Thirumalairaj ◽  
M. Radhakrishnan ◽  
V. Gopikrishnan ◽  
R. Balagurunathan
Keyword(s):  
Plant Growth ◽  
Phosphatase Activity ◽  
Rhizosphere Soil ◽  
Phosphate Solubilization ◽  
Control Plant ◽  
Agar Plate ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Background: Phosphorus has been considered as the key element as it is directly involved in most of the life processes including in plants. Soil contains both organic and inorganic forms of phosphorus. Phosphate solubilization is the important property of soil bacteria to develop them as plant growth promoting bacteria in the agricultural field. The present study report the phosphate solubilizing and plant growth promoting properties of selected actinobacteria isolated from rhizosphere soil.Methods: Actinobacterial strains were isolated from rhizosphere soil and screened for in vitro phosphate solubilizing properties using agar plate method. The efficiency of phosphate solubilization and phosphatase activity of isolated actinobacterial strains were tested using Pikovskaya broth. Result: In the present study phosphate solubilization and phosphatase activity of isolated actinobacteria, Streptomyces sp. CTD-2 was comparatively higher in lab conditions. In pot trial experiment strain CTD-2 showed higher growth when compared to the control plant. The chlorophyll content of leaves in the experimental plants were found maximum with actinobacteria strain CTD-2 treated plant. Plant growth measurements such as root length, shoot length, leaf length, total plant growth measurements, quick yield production were also observed. 

scholarly journals Bacillus subtilis HG-15, a Halotolerant Rhizoplane Bacterium, Promotes Growth and Salinity Tolerance in Wheat (Triticum aestivum)

2020 ◽  
Author(s):  
Chao Ji ◽  
Huimei Tian ◽  
Xiaohui Wang ◽  
Liping Hao ◽  
Changdong Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Salt Stress ◽  
Plant Growth ◽  
Rhizosphere Soil ◽  
Yellow River ◽  
Plant Growth Promoting Bacteria ◽  
Total N ◽  
Available N ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background : Certain plant growth-promoting bacteria (PGPB) reduce salt stress damage in plants. Bacillus subtilis HG-15 is a halotolerant bacterium (able to withstand NaCl concentrations as high as 30%) isolated from the wheat rhizoplane in the Yellow River delta. A qualitative and quantitative investigation of the plant growth-promoting characteristics of this strain confirmed nitrogen fixation, potassium dissolution, and ammonia, plant hormone, 1-aminocyclopropane-1-carboxylic acid deaminase, and proline production abilities. B. subtilis HG-15 colonization of wheat roots, stems, and leaves was examined via scanning electron microscopy , rep-PCR, and double antibiotic screening.Results : Compared with a no B. subtilis HG-15 treatment control, in rhizosphere soil inoculated with the HG-15 strain, the pH (1.08–2.69%), electrical conductivity (3.17–11.48%), and Na + (12.98–15.55%) concentrations significantly decreased ( p < 0.05). Inoculation with the HG-15 strain increased the total N, available N, organic matter, K + , Ca 2+ , and Mg 2+ concentrations in the rhizosphere soil of wheat. Under non-salt stress (0.15% NaCl), low-salt stress (0.25% NaCl), and high-salt stress (0.35% NaCl) conditions, respectively, this strain also significantly increased ( p < 0.05) the dry weight (17.76%, 24.46%, 9.31%), fresh weight (12.80%, 20.48%, 7.43%), plant height (7.79%, 5.86%, 13.13%), root length (10.28%, 17.87%, 48.95%), and other wheat parameters. Through redundancy analysis and Pearson correlation analyses, photosynthesis, biomass accumulation, and osmotic regulation by the wheat plants showed a significant negative correlation with pH, EC, and Na + concentrations in rhizosphere soil.Conclusions : Our results indicated that B . subtilis HG-15 can effectively improve the growth of wheat and elicit induced systemic tolerance in these plant, thus, showing its potential as a microbial inoculant that can protect wheat in salt stress conditions. Furthermore, we determined that the rhizoplane of saline-alkali land plants is an important reservoir for salt-tolerant PGPB.

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

Effect of plant growth promoting bacteria and drought on spring maize (Zea mays L.)

2020 ◽  
Vol 53 (2) ◽  
Author(s):  
Muhammad Mubeen ◽  
Asghari Bano ◽  
Barkat Ali ◽  
Zia Ul Islam ◽  
Ashfaq Ahmad ◽  
...  
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Zea Mays L ◽  
Plant Growth Promoting Bacteria ◽  
Spring Maize ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant Growth Promoting Bacteria (PGPB) - A Versatile Tool for Plant Health Management

2019 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Salah Eddin Khabbaz ◽  
D. Ladhalakshmi ◽  
Merin Babu ◽  
A. Kandan ◽  
V. Ramamoorthy ◽  
...  
Keyword(s):  
Plant Growth ◽  
Health Management ◽  
Plant Health ◽  
Plant Growth Promoting Bacteria ◽  
Versatile Tool ◽  
Plant Growth Promoting ◽  
Growth Promoting
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