scholarly journals Collaborative networks enable the rapid establishment of serological assays for SARS-CoV-2 during nationwide lockdown in New Zealand

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9863 ◽  
Author(s):  
Reuben McGregor ◽  
Alana L. Whitcombe ◽  
Campbell R. Sheen ◽  
James M. Dickson ◽  
Catherine L. Day ◽  
...  
Keyword(s):  
New Zealand ◽  
Respiratory Infections ◽  
Serum Antibody ◽  
Clinical Diagnostics ◽  
Neutralising Antibodies ◽  
Past Infection ◽  
Travel History ◽  
The Relationship ◽  
Collaborative Efforts ◽  
Prior Infection

Background Serological assays that detect antibodies to SARS-CoV-2 are critical for determining past infection and investigating immune responses in the COVID-19 pandemic. We established ELISA-based immunoassays using locally produced antigens when New Zealand went into a nationwide lockdown and the supply chain of diagnostic reagents was a widely held domestic concern. The relationship between serum antibody binding measured by ELISA and neutralising capacity was investigated using a surrogate viral neutralisation test (sVNT). Methods A pre-pandemic sera panel (n = 113), including respiratory infections with symptom overlap with COVID-19, was used to establish assay specificity. Sera from PCR‑confirmed SARS-CoV-2 patients (n = 21), and PCR-negative patients with respiratory symptoms suggestive of COVID-19 (n = 82) that presented to the two largest hospitals in Auckland during the lockdown period were included. A two-step IgG ELISA based on the receptor binding domain (RBD) and spike protein was adapted to determine seropositivity, and neutralising antibodies that block the RBD/hACE‑2 interaction were quantified by sVNT. Results The calculated cut-off (>0.2) in the two-step ELISA maximised specificity by classifying all pre-pandemic samples as negative. Sera from all PCR-confirmed COVID-19 patients were classified as seropositive by ELISA ≥7 days after symptom onset. There was 100% concordance between the two-step ELISA and the sVNT with all 7+ day sera from PCR‑confirmed COVID-19 patients also classified as positive with respect to neutralising antibodies. Of the symptomatic PCR-negative cohort, one individual with notable travel history was classified as positive by two-step ELISA and sVNT, demonstrating the value of serology in detecting prior infection. Conclusions These serological assays were established and assessed at a time when human activity was severely restricted in New Zealand. This was achieved by generous sharing of reagents and technical expertise by the international scientific community, and highly collaborative efforts of scientists and clinicians across the country. The assays have immediate utility in supporting clinical diagnostics, understanding transmission in high-risk cohorts and underpinning longer‑term ‘exit’ strategies based on effective vaccines and therapeutics.

scholarly journals Neutralising antibodies to SARS coronavirus 2 in Scottish blood donors – a pilot study of the value of serology to determine population exposure

2020 ◽  
Author(s):  
C. Thompson ◽  
N. Grayson ◽  
R.S. Paton ◽  
J Lourenco ◽  
BS Penman ◽  
...  
Keyword(s):  
Blood Donors ◽  
Respiratory Infections ◽  
Population Exposure ◽  
Sars Coronavirus ◽  
Neutralising Antibodies ◽  
Past Infection ◽  
Time Period ◽  
History Of ◽  
The Uk

ABSTRACTBackgroundThe extent of spread of SARS coronavirus 2 (SARS-CoV-2) in the UK and elsewhere is unknown because typically only symptomatic individuals are diagnosed. We performed a serological study of recent blood donors in Scotland to detect antibodies to SARS-CoV-2 as a marker of past infection.MethodsA pseudotyped SARS-CoV-2 virus microneutralisation assay was used to detect neutralising antibodies to SARS-CoV-2. The study group comprised samples from 1000 blood donors collected in Scotland during March, 2020. Controls were collected from 100 donors in Scotland during 2019.FindingsAll samples collected on the 17th March, 2020 (n=500) were negative in the pseudotyped SARS-CoV-2 virus microneutralisation assay. Neutralising antibodies were detected in 5 of the 500 samples collected 21st – 23rd March; one further sample was reactive in an anti-spike ELISA.InterpretationAlthough we cannot use the rise in numbers seropositive to infer the contemporary seroprevalence or the growth rate of the epidemic, we note that they are consistent with frequency of reported diagnosed infections and SARS-CoV-2-associated deaths reported in that time period in Scotland, given that seroconversion takes up to 2-3 weeks. It should also be noted that blood donors are not representative of the general population; in particular, those with a history of recent respiratory infections are deferred. Finally, it is unknown what proportion of infected individuals seroconvert and become reactive in the assays used. Serial follow up studies are needed to track infection and seroconversion in this and other similar populations However, these data indicate that sero-surveys of blood banks can serve as a useful tool for tracking the emergence and progression of an epidemic like the current SARS-CoV-2 outbreak.

Australian and New Zealand Biosolids Partnership: Collaboration for more effective biosolids management

2010 ◽  
Vol 5 (2) ◽  
Author(s):  
Andrew Speers ◽  
Allen Gale ◽  
Nancy Penney
Keyword(s):  
New Zealand ◽  
Policy Development ◽  
Sustainable Management ◽  
Best Practice ◽  
Community Consultation ◽  
Critical Research ◽  
Community Attitudes ◽  
Relationship Of ◽  
The Relationship ◽  
Sustainability Framework

This paper describes an international biosolids management initiative, known as the Australian and New Zealand Biosolids Partnership (ANZBP). The ANZBP - known formerly as the Australasian Biosolids Partnership – comprises 33 members dedicated to promoting the sustainable management of biosolids across the two nations. Two critical research projects are described, each of which contributes to the ANZBP goal of promoting the sustainable management of biosolids. The first is a review of community attitudes to biosolids management, the outcomes of which will be used to refine communication tools and methods of community consultation and which will provide input to policy development over time. The second is a review of regulations in place in Australia and New Zealand carried out to identify inconsistencies and improvements that could be made. An outcome of this initiative is potentially the development of a best practice manual. The relationship of the two projects to a sustainability framework adopted by the ANZBP is also described, as is the relationship of the two projects to each other.

scholarly journals An Investigation of the Relationship between Cyniclomyces guttulatus and Rabbit Diarrhoea

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 880
Author(s):  
Tuanyuan Shi ◽  
Xinlei Yan ◽  
Hongchao Sun ◽  
Yuan Fu ◽  
Lili Hao ◽  
...  
Keyword(s):  
New Zealand ◽  
Body Weight Gain ◽  
Animal Experiments ◽  
Zealand White Rabbit ◽  
Opportunistic Pathogen ◽  
Large Numbers ◽  
New Zealand White Rabbits ◽  
Positive Rate ◽  
Relationship Of ◽  
The Relationship

Cyniclomyces guttulatus is usually recognised as an inhabitant of the gastrointestinal (GI) tract in rabbits. However, large numbers of C. guttulatus are often detected in the faeces of diarrhoeic rabbits. The relationship of C. guttulatus with rabbit diarrhoea needs to be clearly identified. In this study, a C. guttulatus Zhejiang strain was isolated from a New Zealand White rabbit with severe diarrhoea and then inoculated into SPF New Zealand white rabbits alone or co-inoculated with Eimeriaintestinalis, another kind of pathogen in rabbits. Our results showed that the optimal culture medium pH and temperature for this yeast were pH 4.5 and 40–42 °C, respectively. The sequence lengths of the 18S and 26S ribosomal DNA fragments were 1559 bp and 632 bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from dogs and 100% homology with the 26S ribosomal sequence of DPA-CGR1 and CGDPA-GP1 isolates from rabbits and guinea pigs, respectively. In animal experiments, the C. guttulatus Zhejiang strain was not pathogenic to healthy rabbits, even when 1 × 108 vegetative cells were used per rabbit. Surprisingly, rabbits inoculated with yeast showed a slightly better body weight gain and higher food intake. However, SPF rabbits co-inoculated with C. guttulatus and E. intestinalis developed more severe coccidiosis than rabbits inoculated with C. guttulatus or E. intestinalis alone. In addition, we surveyed the prevalence of C. guttulatus in rabbits and found that the positive rate was 83% in Zhejiang Province. In summary, the results indicated that C. guttulatus alone is not pathogenic to healthy rabbits, although might be an opportunistic pathogen when the digestive tract is damaged by other pathogens, such as coccidia.

Infection management processes in intensive care and their association with mortality

2021 ◽  
Author(s):  
Leigh P. Fitzpatrick ◽  
Bianca Levkovich ◽  
Steve McGloughlin ◽  
Edward Litton ◽  
Allen C. Cheng ◽  
...  
Keyword(s):  
Intensive Care ◽  
New Zealand ◽  
Hospital Mortality ◽  
Process Of Care ◽  
Infection Management ◽  
Hierarchical Logistic Regression ◽  
Management Processes ◽  
Patient Database ◽  
Improving Patient Care ◽  
The Relationship

Abstract Background ICU-specific tables of antimicrobial susceptibility for key microbial species (‘antibiograms’), antimicrobial stewardship (AMS) programmes and routine rounds by infectious diseases (ID) physicians are processes aimed at improving patient care. Their impact on patient-centred outcomes in Australian and New Zealand ICUs is uncertain. Objectives To measure the association of these processes in ICU with in-hospital mortality. Methods The Australian and New Zealand Intensive Care Society (ANZICS) Adult Patient Database and Critical Care Resources registry were used to extract patient-level factors, ICU-level factors and the year in which each process took place. Descriptive statistics and hierarchical logistic regression were used to determine the relationship between each process and in-hospital mortality. Results The study included 799 901 adults admitted to 173 ICUs from July 2009 to June 2016. The proportion of patients exposed to each process of care was 38.7% (antibiograms), 77.5% (AMS programmes) and 74.0% (ID rounds). After adjusting for confounders, patients admitted to ICUs that used ICU-specific antibiograms had a lower risk of in-hospital mortality [OR 0.95 (99% CI 0.92–0.99), P = 0.001]. There was no association between the use of AMS programmes [OR 0.98 (99% CI 0.94–1.02), P = 0.16] or routine rounds with ID physicians [OR 0.96 (99% CI 0.09–1.02), P = 0.09] and in-hospital mortality. Conclusions Use of ICU-specific antibiograms was associated with lower in-hospital mortality for patients admitted to ICU. For hospitals that do not perform ICU-specific antibiograms, their implementation presents a low-risk infection management process that might improve patient outcomes.

scholarly journals Infliximab is associated with attenuated immunogenicity to BNT162b2 and ChAdOx1 nCoV-19 SARS-CoV-2 vaccines in patients with IBD

Gut ◽  
2021 ◽  
pp. gutjnl-2021-324789
Author(s):  
Nicholas A Kennedy ◽  
Simeng Lin ◽  
James R Goodhand ◽  
Neil Chanchlani ◽  
Benjamin Hamilton ◽  
...  
Keyword(s):  
Single Dose ◽  
Primary Outcome ◽  
Geometric Mean ◽  
Antibody Responses ◽  
Antibody Assay ◽  
Past Infection ◽  
White Ethnicity ◽  
Registration Number ◽  
Inflammatory Bowel ◽  
Prior Infection

ObjectiveDelayed second dose SARS-CoV-2 vaccination trades maximal effectiveness for a lower level of immunity across more of the population. We investigated whether patients with inflammatory bowel disease treated with infliximab have attenuated serological responses to a single dose of a SARS-CoV-2 vaccine.DesignAntibody responses and seroconversion rates in infliximab-treated patients (n=865) were compared with a cohort treated with vedolizumab (n=428), a gut-selective anti-integrin α4β7 monoclonal antibody. Our primary outcome was anti-SARS-CoV-2 spike (S) antibody concentrations, measured using the Elecsys anti-SARS-CoV-2 spike (S) antibody assay 3–10 weeks after vaccination, in patients without evidence of prior infection. Secondary outcomes were seroconversion rates (defined by a cut-off of 15 U/mL), and antibody responses following past infection or a second dose of the BNT162b2 vaccine.ResultsGeometric mean (SD) anti-SARS-CoV-2 antibody concentrations were lower in patients treated with infliximab than vedolizumab, following BNT162b2 (6.0 U/mL (5.9) vs 28.8 U/mL (5.4) p<0.0001) and ChAdOx1 nCoV-19 (4.7 U/mL (4.9)) vs 13.8 U/mL (5.9) p<0.0001) vaccines. In our multivariable models, antibody concentrations were lower in infliximab-treated compared with vedolizumab-treated patients who received the BNT162b2 (fold change (FC) 0.29 (95% CI 0.21 to 0.40), p<0.0001) and ChAdOx1 nCoV-19 (FC 0.39 (95% CI 0.30 to 0.51), p<0.0001) vaccines. In both models, age ≥60 years, immunomodulator use, Crohn’s disease and smoking were associated with lower, while non-white ethnicity was associated with higher, anti-SARS-CoV-2 antibody concentrations. Seroconversion rates after a single dose of either vaccine were higher in patients with prior SARS-CoV-2 infection and after two doses of BNT162b2 vaccine.ConclusionInfliximab is associated with attenuated immunogenicity to a single dose of the BNT162b2 and ChAdOx1 nCoV-19 SARS-CoV-2 vaccines. Vaccination after SARS-CoV-2 infection, or a second dose of vaccine, led to seroconversion in most patients. Delayed second dosing should be avoided in patients treated with infliximab.Trial registration numberISRCTN45176516.

The Impact of Organisational, Social Environmental and Job Content Stressors on the Work Related Strains of Probation Officers

2001 ◽  
Vol 34 (2) ◽  
pp. 193-203 ◽  
Author(s):  
Clare O'donnell ◽  
Christine Stephens
Keyword(s):  
New Zealand ◽  
Health And Safety ◽  
Workplace Stress ◽  
Probation Officers ◽  
Curvilinear Relationship ◽  
Work Stressors ◽  
Work Related ◽  
Job Content ◽  
The Impact ◽  
The Relationship

In recent years workplace stress has been seen as an important occupational health and safety problem and probation officers in New Zealand have been identified as suffering from increasing perceptions of stress. Accordingly, the present study was undertaken with a sample of 50 New Zealand Probation Officers in three offices to examine the relationship of individual, organisational and work stressors with work related strains. It was predicted that work stressors would be positively related to strains and that individual differences (e.g., age or gender) would have a moderating effect on the relationship between stressors and strains. The results showed that stressors caused by organisational problems, such as role boundary and overload, were related to strains, more strongly than job content problems, such as difficult clients. Secondly, age may have a curvilinear relationship to strains. Thirdly, the office, or place of work, moderates the stressor strain relationship.

Clinical Supervision: A Review of Underlying Concepts and Developments

2002 ◽  
Vol 36 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Joanna Macdonald
Keyword(s):  
New Zealand ◽  
American Literature ◽  
Clinical Supervision ◽  
Developmental Process ◽  
Empirical Support ◽  
Personal Contact ◽  
Role Conflicts ◽  
Effective Feedback ◽  
Zealand College ◽  
The Relationship

Objective: This review examines how psychiatric clinical supervision is represented in the psychiatric literature and its relevance for Australasian psychiatry. Method: The literature was first identified then reviewed using Medline and Psychlit, manual searches of relevant journals and personal contact with some key workers in Australia and New Zealand. Results: The predominantly American literature written two to three decades ago reflected the conditions in which psychiatry was practised at that time, largely based in asylums or private offices and informed by the dominant psychoanalytic discourse of that era. These articles, frequently anecdotal and with little empirical support, conceptualized supervision as a developmental process, a syndrome, or a process of identification. They focused substantially on the nature of the relationship between the trainee and supervisor. More recent writers have included trainees’ perspectives. They have identified a number of problems with supervision, including role conflicts, uncertainty about boundaries, lack of supervisory training and lack of effective feedback, and have introduced the concepts of adult learning as highly relevant. These concerns, however, have led to little change to date. Conclusion: The implementation by the Royal Australian and New Zealand College of Psychiatrists (RANZCP) of new training by-laws provides an opportunity to define the meaning of supervision in the current clinical context, to undertake research to clarify the key elements in the process, and to evaluate different techniques of supervision.

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