scholarly journals Engaging inexpensive hands-on activities using Chlamydomonas reinhardtii (a green micro-alga) beads to teach the interplay of photosynthesis and cellular respiration to K4–K16 Biology students

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9817
Author(s):  
Mautusi Mitra ◽  
Sara Michelle Broom ◽  
Kysis Pinto ◽  
Sovi-Mya Doan Wellons ◽  
Ariel Dominique Roberts
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Cellular Respiration ◽  
Successful Implementation ◽  
Biology Students ◽  
Ph Changes ◽  
Laboratory Activities ◽  
Hands On ◽  
Dark Exposure ◽  
Changes Over Time ◽  
Over Time

Background Photosynthesis and cellular respiration play major roles in energy metabolism and are important Life Science topics for K16 Biology students. Algae beads are used for photosynthesis and cellular respiration labs. Currently there are a few companies that sell biology educational kits for making algae beads using non-motile green micro-algae to introduce students to photosynthesis. These kits are expensive and, do not come with detailed guidelines for trouble shooting and customizations for different grade levels. Chlamydomonas reinhardtii is a motile green micro-alga and is an excellent model system for photosynthesis studies. In this article, we are presenting the work conducted in the student-driven, American Society of Plant Biologists-funded, Plant-BLOOME educational outreach project. This project is a supervised collaborative effort of three undergraduates and one high school student. We have generated a protocol which can be used to make Chlamydomonas beads. We have used these beads to design two simple and inexpensive plant biology hands-on activities. These laboratory activities have been customized to teach the interplay of photosynthesis and cellular respiration to K4–K16 Biology students. Methods Chlamydomonas beads were used for two different laboratory activities that involved monitoring pH changes over time using a pH indicator. Our first activity centers on making and, using light-powered algae bead bracelets to monitor dramatic color/pH changes over time when exposed to darkness or light. Our second activity employs strain-specific algae beads with approximately equal cell numbers to conduct comparative photosynthesis and cellular respiration studies in two Chlamydomonas strains namely, wild type, 4A+ and, a high light-sensitive, photosynthetic mutant, 10E35/lsr1a. Results We optimized our experimental protocol using algae beads in a 5.5 mL screw capped glass vials before performing the same experiment in algae bead bracelets. We found that the algal cell density/bead, water type used in the experiment and, the duration of dark exposure of algal beads can affect successful implementation of the lab activities. Light-powered algae bead bracelets showed dramatic color/pH changes within 3 h upon exposure to light or darkness. These bracelets could be switched back and forth between darkness and light multiple times within 48–72 h to display color/pH changes, provided prior dark exposure time did not exceed 9 h. Our comparative studies of photosynthesis and cellular respiration in 10E35 and in 4A+ showed that relative respiration rate and photosynthetic rate is higher and lower in 10E35, respectively, compared to that in 4A+. Additionally, 10E35 failed to display the expected photosynthesis-induced pH/color changes in the light after prolonged exposure to darkness which indicated that prolonged dark exposure of 10E35, hindered photosynthesis.

scholarly journals ONLINE ACCESS TO RESEARCH PAPERS – CHANGES OVER TIME

2018 ◽  
Author(s):  
Julie Burke ◽  
Goeran Fiedler
Keyword(s):  
Data Collection ◽  
Business Models ◽  
Knowledge Generation ◽  
Successful Implementation ◽  
Relevant Research ◽  
Research Papers ◽  
National Assembly ◽  
Online Access ◽  
Changes Over Time ◽  
Over Time

INTRODUCTION The successful implementation of Evidence Based Practice (EBP) depends significantly on practitioners’ access to relevant research articles. It has been argued that the time consuming nature of EBP is a major detriment to its acceptance and widespread use,1 and the logistical difficulties, like having to retrieve research publications of interest from the local university library, are likely to exacerbate this issue. In recognizing the associated adverse effects that this problem could present for knowledge generation and dissemination, the idea to make all research findings publicly available online led to the conception of the world wide web in the early 1990s.2 However, many scientific journals that are slow to abandon their subscription-based business models hide their online content behind paywalls, charging article fees that are usually in the range between $20 and $40. Acknowledging the barriers to EBP that these fees can impose, we have previously compared different strategies for prosthetists and orthotists to maximize their free online access to relevant research literature. The respective data collection in the Spring of 2017 resulted in the finding that approximately 40% of search results in Google Scholar linked to freely available full papers, whereas the remaining 60% links offered only the abstract, but not the full paper, free-of-charge.3 In light of the ever progressing efforts to improve public availability of research, such as the open-access publishing movement or the respective requirements mandated by research funding agencies, we hypothesized that the ratio of freely available online articles is increasing over time, and we repeated our data collection one year after the initial study. Abstract PDF  Link: https://jps.library.utoronto.ca/index.php/cpoj/article/view/32010/24429 How to cite: Burke J, Fiedler G. ONLINE ACCESS TO RESEARCH PAPERS – CHANGES OVER TIME. CANADIAN PROSTHETICS & ORTHOTICS JOURNAL, VOLUME 1, ISSUE 2, 2018, ABSTRACT, POSTER PRESENTATION AT THE AOPA’S 101ST NATIONAL ASSEMBLY, SEPT. 26-29, VANCOUVER, CANADA, 2018.                                                                            Abstracts were Peer-reviewed by the American Orthotic Prosthetic Association (AOPA) 101st National Assembly Scientific Committee.  http://www.aopanet.org/  

scholarly journals Of Heart & Kidneys

2014 ◽  
Vol 76 (8) ◽  
pp. 559-562 ◽  
Author(s):  
Robert M. Kao
Keyword(s):  
Traffic Flow ◽  
Physiological Function ◽  
Organ Development ◽  
Organ Formation ◽  
Cellular Changes ◽  
Hands On ◽  
Evolutionarily Conserved ◽  
Biological Concepts ◽  
Changes Over Time ◽  
Over Time

A major challenge in teaching organ development and disease is deconstructing a complex choreography of molecular and cellular changes over time into a linear stepwise process for students. As an entry toward learning developmental concepts, I propose two inexpensive hands-on activities to help facilitate learning of (1) how to identify defects in heart and kidneys and (2) what evolutionarily conserved strategies from organ development can be applied to understand how to repair these defects. The ease of assembling these activities, combined with traffic flow as a metaphor for physiological function of heart and kidneys, provides students the opportunity to explore and discover biological concepts in organ formation and disease.

Comparison of two devices for measuring exercise transcutaneous oxygen pressures in patients with claudication

VASA ◽  
2015 ◽  
Vol 44 (5) ◽  
pp. 355-362 ◽  
Author(s):  
Marie Urban ◽  
Alban Fouasson-Chailloux ◽  
Isabelle Signolet ◽  
Christophe Colas Ribas ◽  
Mathieu Feuilloy ◽  
...  
Keyword(s):  
Oxygen Pressure ◽  
Optical Sensors ◽  
Chemical Sensors ◽  
Transcutaneous Oxygen ◽  
Starting Values ◽  
Two Systems ◽  
Transcutaneous Oxygen Pressure ◽  
New Sensors ◽  
Changes Over Time ◽  
Over Time

Abstract. Summary: Background: We aimed at estimating the agreement between the Medicap® (photo-optical) and Radiometer® (electro-chemical) sensors during exercise transcutaneous oxygen pressure (tcpO2) tests. Our hypothesis was that although absolute starting values (tcpO2rest: mean over 2 minutes) might be different, tcpO2-changes over time and the minimal value of the decrease from rest of oxygen pressure (DROPmin) results at exercise shall be concordant between the two systems. Patients and methods: Forty seven patients with arterial claudication (65 + / - 7 years) performed a treadmill test with 5 probes each of the electro-chemical and photo-optical devices simultaneously, one of each system on the chest, on each buttock and on each calf. Results: Seventeen Medicap® probes disconnected during the tests. tcpO2rest and DROPmin values were higher with Medicap® than with Radiometer®, by 13.7 + / - 17.1 mm Hg and 3.4 + / - 11.7 mm Hg, respectively. Despite the differences in absolute starting values, changes over time were similar between the two systems. The concordance between the two systems was approximately 70 % for classification of test results from DROPmin. Conclusions: Photo-optical sensors are promising alternatives to electro-chemical sensors for exercise oximetry, provided that miniaturisation and weight reduction of the new sensors are possible.

Neuropsychological Symptoms in Posttraumatic Stress Disorder and Changes Over Time

2007 ◽  
Author(s):  
Miranda Olff ◽  
Mirjam Nijdam ◽  
Kristin Samuelson ◽  
Julia Golier ◽  
Mariel Meewisse ◽  
...  
Keyword(s):  
Posttraumatic Stress Disorder ◽  
Posttraumatic Stress ◽  
Stress Disorder ◽  
Neuropsychological Symptoms ◽  
Changes Over Time ◽  
Over Time

Changes Over Time in Adaptive Behavior Functioning of Twice-Exceptional Children

2010 ◽  
Author(s):  
Rebecca D. Stinson ◽  
Zachary Sussman ◽  
Megan Foley Nicpon ◽  
Allison L. Allmon ◽  
Courtney Cornick ◽  
...  
Keyword(s):  
Adaptive Behavior ◽  
Exceptional Children ◽  
Twice Exceptional ◽  
Changes Over Time ◽  
Over Time
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