scholarly journals Epiphytic bryophyte biomass estimation on tree trunks and upscaling in tropical montane cloud forests

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9351 ◽  
Author(s):  
Guan-Yu Lai ◽  
Hung-Chi Liu ◽  
Ariel J. Kuo ◽  
Cho-ying Huang
Keyword(s):  
Three Dimensional ◽  
Ground Level ◽  
Biomass Estimation ◽  
Cloud Forests ◽  
Study Region ◽  
Conversion Model ◽  
Point Measure ◽  
Stem Surface Area ◽  
Tropical Montane Cloud Forests

Epiphytic bryophytes (EB) are some of the most commonly found plant species in tropical montane cloud forests, and they play a disproportionate role in influencing the terrestrial hydrological and nutrient cycles. However, it is difficult to estimate the abundance of EB due to the nature of their “epiphytic” habitat. This study proposes an allometric scaling approach implemented in twenty-one 30 × 30 m plots across an elevation range in 16,773 ha tropical montane cloud forests of northeastern Taiwan to measure EB biomass, a primary metric for indicating plant abundance and productivity. A general allometry was developed to estimate EB biomass of 100 cm2 circular-shaped mats (n = 131) with their central depths. We developed a new point-intercept instrument to rapidly measure the depths of EB along tree trunks below 300 cm from the ground level (sampled stem surface area (SSA)) (n = 210). Biomass of EB of each point measure was derived using the general allometry and was aggregated across each SSA, and its performance was evaluated. Total EB biomass of a tree was estimated by referring to an in-situ conversion model and was interpolated for all trees in the plots (n = 1451). Finally, we assessed EB biomass density at the plot scale of the study region. The general EB biomass-depth allometry showed that the depth of an EB mat was a salient variable for biomass estimation (R2 = 0.72, p < 0.001). The performance of upscaling from mats to SSA was satisfactory, which allowed us to further estimate mean (±standard deviation) EB biomass of the 21 plots (272 ± 104 kg ha−1). Since a significant relationship between tree size and EB abundance is commonly found, regional EB biomass may be mapped by integrating our method and three-dimensional remotely sensed airborne data.

scholarly journals An allometric scaling approach to estimate epiphytic bryophyte biomass in tropical montane cloud forests

2020 ◽  
Author(s):  
Guan-Yu Lai ◽  
Hung-Chi Liu ◽  
Ariel J. Kuo ◽  
Cho-ying Huang
Keyword(s):  
Allometric Scaling ◽  
Three Dimensional ◽  
Ground Level ◽  
Biomass Estimation ◽  
Cloud Forests ◽  
Study Region ◽  
Conversion Model ◽  
Point Measure ◽  
Stem Surface Area ◽  
Tropical Montane Cloud Forests

AbstractEpiphytic bryophytes (EB) are some of the most commonly found plant species in tropical montane cloud forests, and they play a disproportionate role in influencing the terrestrial hydrological and nutrient cycles. However, it is difficult to estimate the abundance of EB due to the nature of their “epiphytic” habitat. This study proposes an allometric scaling approach to measure EB biomass, implemented in 16,773 ha tropical montane cloud forests of northeastern Taiwan. A general allometry was developed to estimate EB biomass of 100 cm2 circular-shaped mats (n = 131) and their central depths. A point-intercept instrument was invented to measure the depths of EB along tree trunks (n = 210) below 3-m from the ground level (sampled stem surface area [SSA]) in twenty-one 30 × 30 m plots. Biomass of EB of each point measure was derived using the general allometry and was aggregated across each SSA, and its performance was evaluated. Total EB biomass of a tree was estimated by referring to an in-situ conversion model and was interpolated for all trees in the plots (n = 1451). Finally, we assessed EB biomass density at the plot scale and preliminarily estimated EB biomass of the study region. The general EB biomass-depth allometry showed that the depth of an EB mat was a salient variable for biomass estimation (R2 = 0.72, p < 0.001). The performance of upscaling from mats to SSA was satisfactory, which allowed us to further estimate mean (± standard deviation) EB biomass of the 21 plots (272 ± 104 kg ha-1) and to provide preliminary estimation of the total EB biomass of 4562 Mg for the study region. Since a significant relationship between tree size and EB abundance is commonly found, regional EB biomass may be mapped by integrating our method and three-dimensional airborne data.

scholarly journals Biogeography of Tropical Montane Cloud Forests. Part II: Mapping of Orographic Cloud Immersion

2008 ◽  
Vol 47 (8) ◽  
pp. 2183-2197 ◽  
Author(s):  
Udaysankar S. Nair ◽  
Salvi Asefi ◽  
Ronald M. Welch ◽  
D. K. Ray ◽  
Robert O. Lawton ◽  
...  
Keyword(s):  
Costa Rica ◽  
Cloud Forest ◽  
United Nations Environment Programme ◽  
Cloud Base ◽  
Trade Wind ◽  
Cloud Forests ◽  
Study Region ◽  
Modis Data ◽  
Monitoring Centre ◽  
Tropical Montane Cloud Forests

Abstract This study details two unique methods to quantify cloud-immersion statistics for tropical montane cloud forests (TMCFs). The first technique uses a new algorithm for determining cloud-base height using Moderate Resolution Imaging Spectroradiometer (MODIS) cloud products, and the second method uses numerical atmospheric simulation along with geostationary satellite data. Cloud-immersion statistics are determined using MODIS data for March 2003 over the study region consisting of Costa Rica, southern Nicaragua, and northern Panama. Comparison with known locations of cloud forests in northern Costa Rica shows that the MODIS-derived cloud-immersion maps successfully identify known cloud-forest locations in the United Nations Environment Programme (UNEP) World Conservation Monitoring Centre (WCMC) database. Large connected regions of cloud immersion are observed in regions in which the trade wind flow is directly impinging upon the mountain slopes; in areas in which the flow is parallel to the slopes, a fractured spatial distribution of TMCFs is observed. Comparisons of the MODIS-derived cloud-immersion map with the model output show that the MODIS product successfully captures the important cloud-immersion patterns in the Monteverde region of Costa Rica. The areal extent of cloud immersion is at a maximum during morning hours and at a minimum during the afternoon, before increasing again in the evening. Cloud-immersion frequencies generally increase with increasing elevation and tend to be higher on the Caribbean Sea side of the mountains. This study shows that the MODIS data may be used successfully to map the biogeography of cloud forests and to quantify cloud immersion over cloud-forest locations.

scholarly journals Vertical and Horizontal Profiles of Particulate Matter and Black Carbon Near Elevated Highways Based on Unmanned Aerial Vehicle Monitoring

2020 ◽  
Vol 12 (3) ◽  
pp. 1204 ◽  
Author(s):  
Rong Cao ◽  
Bai Li ◽  
Hong-Wei Wang ◽  
Shikang Tao ◽  
Zhong-Ren Peng ◽  
...  
Keyword(s):  
Black Carbon ◽  
Dispersion Model ◽  
Three Dimensional ◽  
Ground Level ◽  
In Situ Monitoring ◽  
Pollution Dispersion ◽  
Vehicle Monitoring ◽  
Aerial Vehicle ◽  
Monitoring Technologies

Highways passing through cities cause additional pollution inside the city. However, most of the current studies are using ground-based monitoring technologies, which make it difficult to capture the dispersion patterns of pollutants near elevated highways or transportation interchanges. The purpose of this study is to discover short-term three-dimensional variations in traffic-related pollutants based on unmanned aerial vehicles. The monitoring locations are at suburban elevated highway and transportation interchanges. The monitoring parameters include the particle number concentration (PN), particle mass concentration (PM), and black carbon (BC). The vertical profiles showed that most air pollutants increased significantly with the height of the elevated highways. Compared with the ground level, PNs increased by 54%–248% and BC increased by 201%. The decline rate of particle concentrations decreased with the increase of height and remained stable after 120 m. Furthermore, the R2 heatmap for regressions between each altitude showed that the linear relationship between 0–120 m was higher than that of other altitudes. In horizontal profiles, PNs spread to 100 m and then began to decline, BC began to decay rapidly after 50 m, but PMs varied less. After crossing another highway, PNs increased by 69–289%, PMs by 7–28%, and BC by 101%. Furthermore, the formation of new particles was observed at both locations as PN3 increased with distance within 100 m from the highway. This paper fills in the void of three-dimensional in situ monitoring near elevated highways, and can help develop and refine a three-dimensional traffic-related air pollution dispersion model and assess the impacts of transportation facilities on the urban environment.

scholarly journals Fine-Scale Ocean Currents Derived From in situ Observations in Anticipation of the Upcoming SWOT Altimetric Mission

2021 ◽  
Vol 8 ◽  
Author(s):  
Bàrbara Barceló-Llull ◽  
Ananda Pascual ◽  
Antonio Sánchez-Román ◽  
Eugenio Cutolo ◽  
Francesco d'Ovidio ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Atlantic Water ◽  
Western Mediterranean ◽  
Fine Scale ◽  
Lagrangian Analysis ◽  
Study Region ◽  
Geostrophic Velocity ◽  
Western Mediterranean Sea ◽  
Ocean Topography

After the launch of the Surface Water and Ocean Topography (SWOT) satellite planned for 2022, the region around the Balearic Islands (western Mediterranean Sea) will be the target of several in situ sampling campaigns aimed at validating the first available tranche of SWOT data. In preparation for this validation, the PRE-SWOT cruise in 2018 was conceived to explore the three-dimensional (3D) circulation at scales of 20 km that SWOT aims to resolve, included in the fine-scale range (1–100 km) as defined by the altimetric community. These scales and associated variability are not captured by contemporary nadir altimeters. Temperature and salinity observations reveal a front that separates local Atlantic Water in the northeast from recent Atlantic Water in the southeast, and extends from the surface to ~150 m depth with maximum geostrophic velocities of the order of 0.20 m s−1 and a geostrophic Rossby number that ranges between −0.24 and 0.32. This front is associated with a 3D vertical velocity field characterized by an upwelling cell surrounded by two downwelling cells, one to the east and the other to the west. The upwelling cell is located near an area with high nitrate concentrations, possibly indicating a recent inflow of nutrients. Meanwhile, subduction of chlorophyll-a in the western downwelling cell is detected in glider observations. The comparison of the altimetric geostrophic velocity with the CTD-derived geostrophic velocity, the ADCP horizontal velocity, and drifter trajectories, shows that the present-day resolution of altimetric products precludes the representation of the currents that drive the drifter displacement. The Lagrangian analysis based on these velocities demonstrates that the study region has frontogenetic dynamics not detected by altimetry. Our results suggest that the horizontal component of the flow is mainly geostrophic down to scales of 20 km in the study region and during the period analyzed, and should therefore be resolvable by SWOT and other future satellite-borne altimeters with higher resolutions. In addition, fine-scale features have an impact on the physical and biochemical spatial variability, and multi-platform in situ sampling with a resolution similar to that expected from SWOT can capture this variability.

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

In situ investigation of the primary recrystallization of alpha titanium in HVEM

1978 ◽  
Vol 36 (1) ◽  
pp. 634-635
Author(s):  
S. Naka ◽  
R. Penelle ◽  
R. Valle
Keyword(s):  
Dimensional Analysis ◽  
Texture Evolution ◽  
Cold Work ◽  
Three Dimensional ◽  
Primary Recrystallization ◽  
Thin Foils ◽  
In Situ Investigation ◽  
Grain Growth Model ◽  
Alpha Titanium

The in situ experimentation technique in HVEM seems to be particularly suitable to clarify the processes involved in recrystallization. The material under investigation was unidirectionally cold-rolled titanium of commercial purity. The problem was approached in two different ways. The three-dimensional analysis of textures was used to describe the texture evolution during the primary recrystallization. Observations of bulk-annealed specimens or thin foils annealed in the microscope were also made in order to provide information concerning the mechanisms involved in the formation of new grains. In contrast to the already published work on titanium, this investigation takes into consideration different values of the cold-work ratio, the temperature and the annealing time.Two different models are commonly used to explain the recrystallization textures i.e. the selective grain growth model (Beck) or the oriented nucleation model (Burgers). The three-dimensional analysis of both the rolling and recrystallization textures was performed to identify the mechanismsl involved in the recrystallization of titanium.

Detection and mapping of interactions between chromatin and the nuclear envelope in drosophila embryos

1995 ◽  
Vol 53 ◽  
pp. 764-765
Author(s):  
W.F. Marshall ◽  
A.F. Dernburg ◽  
B. Harmon ◽  
J.W. Sedat
Keyword(s):  
Nuclear Envelope ◽  
Chromatin Condensation ◽  
Three Dimensional ◽  
Physiological Role ◽  
Nuclear Surface ◽  
Intact Cells ◽  
Molecular Determinants ◽  
Surface Harmonic ◽  
Drosophila Embryos

Interactions between chromatin and nuclear envelope (NE) have been implicated in chromatin condensation, gene regulation, nuclear reassembly, and organization of chromosomes within the nucleus. To further investigate the physiological role played by such interactions, it will be necessary to determine which loci specifically interact with the nuclear envelope. This will not only facilitate identification of the molecular determinants of this interaction, but will also allow manipulation of the pattern of chromatin-NE interactions to probe possible functions. We have developed a microscopic approach to detect and map chromatin-NE interactions inside intact cells.Fluorescence in situ hybridization (FISH) is used to localize specific chromosomal regions within the nucleus of Drosophila embryos and anti-lamin immunofluorescence is used to detect the nuclear envelope. Widefield deconvolution microscopy is then used to obtain a three-dimensional image of the sample (Fig. 1). The nuclear surface is represented by a surface-harmonic expansion (Fig 2). A statistical test for association of the FISH spot with the surface is then performed.

Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

1996 ◽  
Vol 54 ◽  
pp. 288-289
Author(s):  
Greg V. Martin ◽  
Ann L. Hubbard
Keyword(s):  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Polarized Secretion ◽  
Microscope Images ◽  
Computer Aided ◽  
Intracellular Organelles ◽  
Cytoskeletal Elements ◽  
Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

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