scholarly journals Aquatic insect community structure revealed by eDNA metabarcoding derives indices for environmental assessment

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9176
Author(s):  
Noriko Uchida ◽  
Kengo Kubota ◽  
Shunsuke Aita ◽  
So Kazama
Keyword(s):  
Survey Data ◽  
Environmental Assessment ◽  
Aquatic Insects ◽  
Nitrogen Concentration ◽  
Aquatic Insect ◽  
Ecological Status ◽  
Organic Pollution ◽  
Taxonomic Resolution ◽  
Survey Method ◽  
Insect Community

Environmental DNA (eDNA) analysis provides an efficient and objective approach for monitoring and assessing ecological status; however, studies on the eDNA of aquatic insects, such as Ephemeroptera, Plecoptera, and Trichoptera (EPT), are limited despite its potential as a useful indicator of river health. Here, we investigated the community structures of aquatic insects using eDNA and evaluated the applicability of eDNA data for calculating assessment indices. Field surveys were conducted to sample river water for eDNA at six locations from upstream to downstream of two rivers in Japan in July and November 2016. Simultaneously, aquatic insects were collected using the traditional Surber net survey method. The communities of aquatic insects were revealed using eDNA by targeting the cytochrome oxidase subunit I gene in mitochondrial DNA via metabarcoding analyses. As a result, the eDNA revealed 63 families and 75 genera of aquatic insects, which was double than that detected by the Surber net survey (especially for families in Diptera and Hemiptera). The seasonal differences of communities were distinguished by both the eDNA and Surber net survey data. Furthermore, the total nitrogen concentration, a surrogate of organic pollution, showed positive correlations with biotic environmental assessment indices (i.e., EPT index and Chironomidae index) calculated using eDNA at the genus-level resolution but the indices calculated using the Surber net survey data. Our results demonstrated that eDNA analysis with higher taxonomic resolution can provide as a more sensitive environmental assessment index than the traditional method that requires biotic samples.

Spatial and seasonal variability of emergent aquatic insects and nearshore spiders in a subtropical estuary

2019 ◽  
Vol 70 (4) ◽  
pp. 541 ◽  
Author(s):  
Martha J. Zapata ◽  
S. Mažeika P. Sullivan
Keyword(s):  
Aquatic Insects ◽  
High Salinity ◽  
Aquatic Insect ◽  
Freshwater Ecosystems ◽  
Dispersal Ability ◽  
Insect Community ◽  
Insect Communities ◽  
Low Salinity ◽  
Subtropical Estuary ◽  
Adult Aquatic Insects

Variability in the density and distribution of adult aquatic insects is an important factor mediating aquatic-to-terrestrial nutritional subsidies in freshwater ecosystems, yet less is understood about insect-facilitated subsidy dynamics in estuaries. We surveyed emergent (i.e. adult) aquatic insects and nearshore orb-weaving spiders of the families Tetragnathidae and Araneidae in a subtropical estuary of Florida (USA). Emergent insect community composition varied seasonally and spatially; densities were lower at high- than low-salinity sites. At high-salinity sites, emergent insects exhibited lower dispersal ability and a higher prevalence of univoltinism than low- and mid-salinity assemblages. Orb-weaving spider density most strongly tracked emergent insect density rates at low- and mid-salinity sites. Tetragnatha body condition was 96% higher at high-salinity sites than at low-salinity sites. Our findings contribute to our understanding of aquatic insect communities in estuarine ecosystems and indicate that aquatic insects may provide important nutritional subsidies to riparian consumers despite their depressed abundance and diversity compared with freshwater ecosystems.

scholarly journals Assessment of Water Quality Parameters and Aquatic Insect Assemblages in Dalvoy Lake, Mysore District, Karnataka, India.

2021 ◽  
Vol 16 (2) ◽  
pp. 514-529
Author(s):  
Hebbal Rajendra Abhilash ◽  
Malliah Mahadevaswamy
Keyword(s):  
Water Quality ◽  
Aquatic Insects ◽  
Aquatic Insect ◽  
Well Being ◽  
Quality Parameters ◽  
Shannon Index ◽  
Water Quality Parameters ◽  
Quality Analysis ◽  
Total Hardness ◽  
Insect Community

Water is an indispensable natural resource vital for the survival of all life forms. It contributes significantly to the country's economic prosperity and general well-being. As a result, understanding the status of water bodies is crucial to assure their long-term use. A study on water quality parameters and aquatic insect community was therefore carried on the surface waters of Dalvoy Lake which is spread over an area of 133.43 acres and having a length of 2.2 km. Monthly water samples were collected using one-litre labeled plastic containers from three locations between the hours of 8 a.m. and 10 a.m. Water quality analysis was conducted following standard methods and compared to drinking water specifications. The dissolved oxygen content, total hardness, conductivity, total dissolved solids, and turbidity exceeded the permissible limits. Aquatic insects were collected from the same locations and preserved in 4% formalin. A total of 15 species of aquatic insects belonging to four orders, Hemiptera, Coleoptera, Diptera, and Ephemeroptera, were identified in this study. The Hemiptera was shown to be the largest group comprising of 8 species; 4 species of Diptera; 2 species of Coleoptera and a lone species of Ephemeroptera. The computation of aquatic insect dominance status using Engelmann's Scale revealed Diplonychus rusticus, Anisops sp., Enithares sp., Chironomous sp. and Culex sp. as dominant. Biodiversity metrics like the Shannon Index (1.4-2.11) and the evenness index (0.50-0.66) reflect the agitation of the system. Based on the results of the water quality index (163.67) and aquatic insect assemblage, it is evident that the water in Dalvoy Lake is deteriorated and is unfit for domestic usage. The study also indicated the abundance of two genera Chironomous and Culex of the Diptera order which are the index of water pollution. Thus, the present study calls for urgent and strict vigilance and continuous monitoring of this perennial water body for conservation and sustainable management.

scholarly journals Diet and trophic groups of an aquatic insect community in a tropical stream

2004 ◽  
Vol 64 (4) ◽  
pp. 809-817 ◽  
Author(s):  
R. L. Motta ◽  
V. S. Uieda
Keyword(s):  
Organic Matter ◽  
Seasonal Variation ◽  
Aquatic Insects ◽  
Community Organization ◽  
Aquatic Insect ◽  
Limited Resource ◽  
Trophic Groups ◽  
Insect Community ◽  
Tropical Stream ◽  
Feeding Specialization

The diet and trophic groups of an assemblage of aquatic insects were studied in a tropical stream. Genera of the orders Ephemeroptera, Odonata, Plecoptera, Lepidoptera, and Hemiptera showed feeding specialization. Others, such as Trichoptera, Coleoptera, and Diptera, showed great diet variation with genera of different trophic groups. Seasonal variation of insect diet, evident only for some genera of the orders Trichoptera, Lepidoptera, Coleoptera, and Diptera, was due to the differences observed in community composition and to generalist habits of these genera. However, the seasonal comparison of trophic groups showed no significant statistical differences. The great importance of organic matter, a non-limited resource, in the diet of Ribeirão do Atalho aquatic insects may be the explanation for the trophic stability in this community organization.

scholarly journals Species richness and diversity of aquatic insects inhabiting rice fields in Bogor, West Java, Indonesia

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Wakhid Wakhid ◽  
Aunu Rauf ◽  
Majariana Krisanti ◽  
I Made Sumertajaya ◽  
Nina Maryana
Keyword(s):  
Species Richness ◽  
Aquatic Insects ◽  
Agricultural Landscape ◽  
Group Analysis ◽  
Aquatic Insect ◽  
Abundant Species ◽  
Rice Fields ◽  
Insect Community ◽  
West Java ◽  
Species Richness And Diversity

Abstract. Wakhid, Rauf A, Krisanti M, Sumertajaya IM, Maryana N. 2020. Species richness and diversity of aquatic insects inhabiting rice fields in Bogor, West Java, Indonesia. Biodiversitas 21: 34-42. Rice fields occupy the largest cultivated area in agricultural landscape in Indonesia and support a variety of living organisms, including aquatic insects. This study was conducted with the objective to determine the species richness and diversity of aquatic insects inhabiting rice fields. Sampling was made in March, May, and July 2017 in rice fields at Situgede, Pandansari, and Kawungluwuk (Bogor region, West Java, Indonesia), and carried out by dragging dip net on the bottom along the edge of the rice plots. A total of 3,306 individuals representing 45 species of aquatic insects belonging to 30 genera, 20 families, and seven orders were recorded. Order Hemiptera was the most abundance comprising 28.89% of the total insects collected, followed by Diptera (24.80%), Coleoptera (24.41%), and Odonata (21.42%). Functional feeding group analysis showed that collectors-gatherers had the highest proportion (40 - 46%), followed by predators (23-44%) and scrapers (10-35%). Rank-abundance curve showed low species evenness with the four most abundant species were Micronecta siva (Kirkaldy) (Hemiptera: Micronectidae), Chironomus sp. (Diptera: Chironomidae), Orthetrum sabina (Drury) (Odonata: Libellulidae), and Helochares sp. (Coleoptera: Hydrophilidae). The Shannon-Wiener index showed the lowest value (H’=1.84) at Situgede and the highest (H’=2.05) at Pandansari. Richness estimate and individual-based rarefaction curve revealed that rice fields at Pandansari have more species richness than the other two sites. This study provides some insights into the aquatic insect community of the human-made ecosystem and suggests that the ecological approach to pest management is necessary for maintaining ecosystem health and promoting biodiversity.

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