scholarly journals The role and proteomic analysis of ethylene in hydrogen gas-induced adventitious rooting development in cucumber (Cucumis sativus L.) explants

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8896 ◽  
Author(s):  
Dengjing Huang ◽  
Biting Bian ◽  
Meiling Zhang ◽  
Chunlei Wang ◽  
Changxia Li ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Adventitious Rooting ◽  
Hydrogen Gas ◽  
Mrna Levels ◽  
Related Protein ◽  
Cucumis Sativus L ◽  
Downstream Signaling ◽  
Biological Responses ◽  
Cytosolic Ascorbate Peroxidase ◽  
Oxygen Evolving

Previous studies have shown that both hydrogen gas (H2) and ethylene (ETH) play positive roles in plant adventitious rooting. However, the relationship between H2and ETH during this process has not been explored and remains insufficiently understood. In this study, cucumber (Cucumis sativus L.) was used to explore the proteomic changes in ETH-H2-induced rooting. Our results show that hydrogen-rich water (HRW) and ethylene-releasing compound (ethephon) at proper concentrations promote adventitious rooting, with maximal biological responses occurring at 50% HRW or 0.5 µM ethephon. ETH inhibitors aminoethoxyvinylglycine (AVG) and AgNO3 cause partial inhibition of adventitious rooting induced by H2, suggesting that ETH might be involved in H2-induced adventitious rooting. According to two-dimensional electrophoresis (2-DE) and mass spectrometric analyses, compared with the control, 9 proteins were up-regulated while 15 proteins were down-regulated in HRW treatment; four proteins were up-regulated while 10 proteins were down-regulated in ethephon treatment; and one protein was up-regulated while nine proteins were down-regulated in HRW+AVG treatment. Six of these differentially accumulated proteins were further analyzed, including photosynthesis -related proteins (ribulose-1,5-bisphosphate carall boxylase smsubunit (Rubisco), sedoheptulose-1,7-bisphosphatase (SBPase), oxygen-evolving enhancer protein (OEE1)), amino and metabolism-related protein (threonine dehydratase (TDH)), stress response-related protein (cytosolic ascorbate peroxidase (CAPX)), and folding, modification and degradation-related protein (protein disulfide-isomerase (PDI)). Moreover, the results of real-time PCR about the mRNA levels of these genes in various treatments were consistent with the 2-DE results. Therefore, ETH may be the downstream signaling molecule during H2- induced adventitious rooting and proteins Rubisco, SBPase, OEE1, TDH, CAPX and PDI may play important roles during the process.

scholarly journals Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber (Cucumis sativus L.) explants

2019 ◽  
Author(s):  
Li Yutong ◽  
Yue Wu ◽  
Weibiao Liao ◽  
Linli Hu ◽  
Mohammed Mujitaba Dawuda ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cucumis Sativus ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Adventitious Rooting ◽  
Adventitious Root Formation ◽  
No Donor ◽  
Cucumis Sativus L ◽  
Positive Effects ◽  
Relative Expression Level

Abstract Background: Brassinolide (BR), as a new type of plant hormones, is involved in the process of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. In my study, the main purpose is to explore whether nitric oxide (NO) is involved in the process of BR-induced adventitious root formation in cucumber (Cucumis sativus L.), and whether it plays a certain role. Results: Exogenous application of 1 μM BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 μM) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP mixed treatment significantly promoted adventitious rooting and the promoted effects was significantly superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 μM also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR. Conclusion: BR promoted the formation of adventitious roots via inducing the generation of endogenous NO.

Hydrogen peroxide is involved in abscisic acid-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress

2016 ◽  
Vol 59 (5) ◽  
pp. 536-548 ◽  
Author(s):  
Xue-Ping Li ◽  
Qing-Qing Xu ◽  
Wei-Biao Liao ◽  
Zhan-Jun Ma ◽  
Xiao-Ting Xu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Abscisic Acid ◽  
Drought Stress ◽  
Cucumis Sativus ◽  
Adventitious Rooting ◽  
Cucumis Sativus L
Sign in / Sign up

Export Citation Format

Share Document