scholarly journals Responses of rhizosphere soil bacteria to 2-year tillage rotation treatments during fallow period in semiarid southeastern Loess Plateau

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8853
Author(s):  
Qing Xia ◽  
Xiaoli Liu ◽  
Zhiqiang Gao ◽  
Jianming Wang ◽  
Zhenping Yang

Background Soil compaction can be mitigated by deep tillage and subsoiling practices following a long period of no-tillage. Fallow tillage rotation methods are frequently used to improve water availability in the soils of the southeastern Loess Plateau region of China. Rhizosphere soil bacteria are ecologically important for the transformation of matter and energy in the plant root system and can be influenced by tillage rotation treatments. However, the effect of tillage rotations on the bacterial community and structure of rhizosphere soil is not well understood. Methods A two-year field experiment was conducted with four tillage rotation treatments, including subsoil–subsoil (SS-SS), subsoil–deep tillage (SS-DT), deep tillage–deep tillage (DT-DT), and the control treatment of no-tillage–no-tillage (NT-NT). Our study was conducted during wheat’s fallow period to investigate the abundance, diversity, and functions of rhizosphere soil bacteria using high-throughput sequencing technology. Results Our results showed that tillage rotation methods significantly influenced the bacterial diversity and composition of the rhizosphere soil in the plough layer (20–40 cm depth) by altering the moisture content of the soil. The metabolism, environmental information processing, and genetic information processing of the bacteria in the rhizosphere soil were affected. The most abundant phyla across all samples were Proteobacteria, Actinobacteria, Acidobacteria, Planctomycetes, Bacteroidetes, Gemmatimonadetes, Frimicutes, Chloroflexi, Nitrospirae, and Verrucomicrobia, which are classic bacterial decomposers in soil. The bacterial diversity and composition was similar for treatments causing higher soil perturbation (SS-DT and DT-DT), which disrupted the balance between aerobic and anaerobic bacteria. The less disruptive tillage methods (SS-SS and NT-NT), preserved the integrity of the soil bacteria. However, the NT-NT treatment may have led to soil compaction, particularly in the 20–40 cm layer. These results suggested that SS-SS was the most effective tillage rotation practice to accumulate soil moisture, maintain the balance between aerobic and anaerobic bacteria, and to enhance the metabolic capacity of rhizosphere soil bacteria. This method may have a significant impact on the sustainable development and farming practices of dryland agriculture.

1983 ◽  
Vol 15 (5) ◽  
pp. 129-135 ◽  
Author(s):  
Z Filip ◽  
K Seidel ◽  
H Dizer

To determine whether long-term sewage treatment can take place without a pollution risk for soil and groundwater, samples from sewage irrigation fields in West Berlin which have been in use since about 1890 were analyzed for enteric viruses and microorganisms. Enteric viruses were detected in only seven samples from a total number of eighty seven taken from different soil depths. With only one exception, no viruses were found below 60 cm. No viruses were detected in groundwater samples. Long-term sewage irrigation did not result in significant changes in the colony courts of aerobic soil bacteria, but the counts of anaerobic bacteria and actinomycetes were slightly elevated and those of microscopic fungi slightly decreased. Potentially pathogenic bacteria were not detected in soil below 90 cm.


1982 ◽  
Vol 93 (sup386) ◽  
pp. 100-102 ◽  
Author(s):  
J. Luotonen ◽  
A. M. M. Jokipii ◽  
P. Sipilä ◽  
J. Väyrynen ◽  
L. Jokipii ◽  
...  

2018 ◽  
Vol 52 (4) ◽  
pp. 325-329
Author(s):  
Ekachai Chukeatirote ◽  
Thanong Phueaouan ◽  
Anong Piwkam

1988 ◽  
Vol 16 (2) ◽  
pp. 98-106 ◽  
Author(s):  
C. Matula ◽  
M. Hildebrandt ◽  
G. Nahler

Six preparations (four liquid and two solid) were tested in a double-blind crossover design for their anti-bacterial effect on aerobic and anaerobic bacteria in saliva of 12 volunteers. The four liquid preparations contained either tyrothricin, hexetidine, hydrogen peroxide or ethanol and were tested against a rinse with water as control. The two solid preparations, in the form of lozenges, contained tyrothricin in doses of 4 or 10 mg and were tested against parafilm. A single rinse with either tyrothricin or hexetidine resulted in a significant reduction of aerobic and anaerobic bacteria in saliva which lasted for the whole 1h observation period and was considerably more effective than hydrogen peroxide or ethanol. Similar results were seen with the solid preparations. The lozenge containing 10 mg tyrothricin was the most effective and was the only formulation capable of reducing oral aerobic bacterial counts by a factor of approximately 100.


1999 ◽  
Vol 43 (4) ◽  
pp. 738-744 ◽  
Author(s):  
P. J. Petersen ◽  
N. V. Jacobus ◽  
W. J. Weiss ◽  
P. E. Sum ◽  
R. T. Testa

ABSTRACT The 9-t-butylglycylamido derivative of minocycline (TBG-MINO) is a recently synthesized member of a novel group of antibiotics, the glycylcyclines. This new derivative, like the first glycylcyclines, theN,N-dimethylglycylamido derivative of minocycline and 6-demethyl-6-deoxytetracycline, possesses activity against bacterial isolates containing the two major determinants responsible for tetracycline resistance: ribosomal protection and active efflux. The in vitro activities of TBG-MINO and the comparative agents were evaluated against strains with characterized tetracycline resistance as well as a spectrum of recent clinical aerobic and anaerobic gram-positive and gram-negative bacteria. TBG-MINO, with an MIC range of 0.25 to 0.5 μg/ml, showed good activity against strains expressing tet(M) (ribosomal protection), tet(A), tet(B),tet(C), tet(D), and tet(K) (efflux resistance determinants). TBG-MINO exhibited similar activity against methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant streptococci, and vancomycin-resistant enterococci (MICs at which 90% of strains are inhibited, ≤0.5 μg/ml). TBG-MINO exhibited activity against a wide diversity of gram-negative aerobic and anaerobic bacteria, most of which were less susceptible to tetracycline and minocycline. The in vivo protective effects of TBG-MINO were examined against acute lethal infections in mice caused by Escherichia coli, S. aureus, andStreptococcus pneumoniae isolates. TBG-MINO, administered intravenously, demonstrated efficacy against infections caused byS. aureus including MRSA strains and strains containingtet(K) or tet(M) resistance determinants (median effective doses [ED50s], 0.79 to 2.3 mg/kg of body weight). TBG-MINO demonstrated efficacy against infections caused by tetracycline-sensitive E. coli strains as well asE. coli strains containing either tet(M) or the efflux determinant tet(A), tet(B), ortet(C) (ED50s, 1.5 to 3.5 mg/kg). Overall, TBG-MINO shows antibacterial activity against a wide spectrum of gram-positive and gram-negative aerobic and anaerobic bacteria including strains resistant to other chemotherapeutic agents. The in vivo protective effects, especially against infections caused by resistant bacteria, corresponded with the in vitro activity of TBG-MINO.


2018 ◽  
Vol 30 (3) ◽  
pp. 13-16
Author(s):  
Khudair A Abd ◽  
Basima G Ali ◽  
Abbas S AL-Mizraqchi

Back ground: Microbial penetration inside the implant's internal hole creates a bacterial reservoir that is related with an area of inflamed connective tissue opposite the fixture-abutment junction and this can affect the health of the peri-implant tissue. Aims of the study: Evaluate the types aerobic and anaerobic bacterial count-percentage and difference between Aerobic and Anaerobic microflora in the implant screw hole three months after implant placement. Monitor the periodontal health status of all patients, throughout the study. Material and methods: Study methodology; Eight partially edentulous patients received 20 dental implants and these implants done with flapless surgical procedure. All patients examined clinically to determined their oral health status by examination of their plaque index, Gingival index and Bleeding on probing, each two weeks for 90 days (8visits) throughout the study period. Three months after that, the plaque sample collected from the internal hole of fixture and transfer to bacterial investigation and assessment the amount of anaerobic and aerobic bacteria. Results: Although the anaerobic viable count is higher than that of aerobic, but with statistically not significant difference between those counts (P>0.05). Keywords: screw hole, flapless surgical implant placement, bacterial count, Aerobic and Anaerobic bacteria


2018 ◽  
Vol 49 (2) ◽  
pp. 110
Author(s):  
S. RANTOS (Σ. ΡΑΝΤΟΣ) ◽  
L. PANTOULAS (Λ. ΠΑΝΤΟΥΛΑΣ) ◽  
I. SARAKATSIANOS (Ι. ΣΑΡΑΚΑΤΣΙΑΝΟΣ) ◽  
G. ROZOS (Γ. ΡΟΖΟΣ) ◽  
N. PAPAIOANNOU (Ν. ΠAΠΑΪΩΑNNOΥ)

During this study canned food for dogs of six firms were investigated (42 samples totally). Microbiologically the following parameters were checked: the commercial sterility, the water activity (aw), the pH, the presence of aerobic and anaerobic bacteria after incubation of tins in 25 ° C for 28 days, 32 ° C for 21 days and 55 ' C for 8 days. Histologically, the existence of different kinds of tissues was examined. Technologically Weende's analysis and separation of the tin's content were made. Toxicologically, the concentration of lead and cadmium were countered. The results showed that dog's canned food, in Greece, are supersterillised. Muscular, adipose and connective tissue are used for their production as well as byproducts and soya proteins by some firms. The results of Weende's analysis are in full agreement with the amount of nutriments that they were written on tins' labels.


2018 ◽  
pp. 6-17
Author(s):  
І. Prymak ◽  
А. Panchenko ◽  
M. Voitovyk ◽  
V. Karpenko ◽  
S. Levandovska ◽  
...  

The evolutional way of developing of the theoretical and practical basis of main tillage since the middle of the first part of the 20th century up today is highlighted. Exaggerated ideologization of science, government monopoly of academician V.R. Williams from thirties to fifties years of the 20th century caused big losses in agrarian science, especially in farming and agriculture sciences. The disaster of Williams was caused by absolute priority of soil conditioning and universalization of developed by him grass rotation system of farming. Till fifties in crop farming arable tillage to the depth at least 20–22 cm dominated completely. The first person in the USSR to refuse from a plough was T.S. Maltsev who recommended to hold deep beardless tillage once in 4–5 years, and for the rest of the time to use surface one (up to 8 cm) or surface tillage (10–12 cm) with discs. After T.S. Maltsev works, the issues of differentiation of tilled soil layers under beardless and surface tillage became especially controversial. The final boost for the development of theoretical and practical basis of tillage minimization was soil protecting system suggested by the group of scientists of the former All-Soviet Union Research Institute of grain growing headed by O.І. Baraievyi. It was based on beardless tillage, sowing with stubbly seeding machines, flap and buffer planting, snow capture etc. Early adopters of a full refusal from beard tillage in Ukraine during 80–90ies years were І.Ye. Shcherbak, F.M. Morgun, M.K. Shykula, S.S. Antonets and their students and followers. Nowadays the majority of national scientists consider the differential different depth tillage as the most effective one during crop rotations, which includes alternation of different ways, events and means of soil tillage depending on ecological conditions, crops biological features, structure of crop acreage, fertilization systems and plants protection etc. In scientists opinion, the minimal tillage is available on 3 millions of hectare and no-tillage is available on 5,49 millions of hectare out of 30 millions of tilled soils in Ukraine. In thirties V.R. Williams and M.S. Sokolov criticized surface tillage. Academician M.M. Tulaykov who was openly against doctrines of V.R. Williams recommended surface tillage in dry regions which in his opinion had to protect tilled soil from wind formed processes. He claimed that surface tillage is economically suitable for poor husbandries and that dust storms are the result of a constant daily soil drifting caused by deep tillage. In forties an American farmer E. Folkner widely promoted surface tillage together with soil mulching with plant matters of green crops. The first person to refuse from beard tillage in the first part of the 20th century in the Soviet empire was T.S. Maltsev. He proved decisively the advantages of beardless tillage over beard one in Transurals. T.S. Maltsev suggested the whole range of tools for soil tillage developed by him, the main of which were cultrate pulverizers and a beardless plough. The necessity of tillage with plough was the main stereotype existing in the world tillage theory and practice till the fifties. Scientific inheritance of M.M. Tulaykov became one of the bases for reconsideration of the concept of V.R. Williams existing in Ukraine. He concluded that it was necessary to change beard tillage to surface one to the depth 10-12 cm under which root system of one-year-old plants would be decomposed in tight soil where anoxic conditions must dominate. And to cultivate bottom layers it was necessary to hold deep tillage with beardless ploughs once in 4–5 years. The first person to refuse from a plough in the USSR was T.S. Maltsev. He suggested using surface plowing with disc tillers and deep beardless tillage (40–50 cm) once in 4–5 years instead of tillage in crops rotation of Transural. In 1952 he made a beardless plough for the first time. The most controversial issue among the scientists of Ukraine was differentiation of the tilled layer and its effect on crop productivity under beardless and surface tillage. In sixties and seventies O.I. Baraiev and his colleagues from All Soviet Union Research Institute of grain farming headed by him developed for the first time a soil protective system based exceptionally on different depth beardless tillage in crop rotations, usage of stubbly seeding machines, subsurface cultivators, soil spikers, coulisse fallow, buffer planting etc. In Ukraine during eighties and nineties years a complete refusal from beard tillage in favor of beardless tillage was supported by І.Ye. Shcherbak, F.T. Morgun, M.K. Shykula, S.S. Antonets and other scientists and experts. High weed infestation of fields under such tillage was the main obstacle to its widespread usage. In the 21st century the majority of scientists recommend differential different depth main tillage which suggests different ways, events and means of its implementation in crop rotations depending on biological features of crop, soil and climate conditions, fertilizing systems, plant protection etc, 13 millions hectare of tilled soil is technologically suitable for minimum tillage in Ukraine, and the rest 5,5 million hectare do not even need tillage at all. Key words: tillage, soil, evolution, plough, subsurface cultivator, erosion, minimization, differentiation.


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