scholarly journals Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8716
Author(s):  
Lihua Xie ◽  
Miaomiao Cai ◽  
Xiangyu Li ◽  
Huifang Zheng ◽  
Yali Xie ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Leaf Senescence ◽  
Stress Responses ◽  
Abiotic Stress Tolerance ◽  
Moso Bamboo ◽  
Biotic And Abiotic Stress ◽  
Phyllostachys Edulis ◽  
Salt And Drought Stress ◽  
Nac Family

The NAC family is one of the largest transcription factor families unique to plants, which regulates the growth and development, biotic and abiotic stress responses, and maturation and senescence in plants. In this study, PheNAC3, a NAC gene, was isolated and characterized from moso bamboo (Phyllostachys edulis). PheNAC3 belong to the NAC1 subgroup and has a conserved NAC domain on the N-terminus, which with 88.74% similarity to ONAC011 protein. PheNAC3 localized in the nucleus and exhibited transactivation activity. PheNAC3 was upregulated during the process of senescence of leaves and detected shoots. PheNAC3 was also induced by ABA, MeJA, NaCl and darkness, but it had no remarkable response to PEG and SA treatments. Overexpression of PheNAC3 could cause precocious senescence in Arabidopsis. Transgenic Arabidopsis displayed faster seed germination, better seedling growth, and a higher survival rate than the wild-type under salt or drought stress conditions. Moreover, AtSAG12 associated with senescence and AtRD29A and AtRD29b related to ABA were upregulated by PheNAC3 overexpression, but AtCAB was inhibited. These findings show that PheNAC3 may participate in leaf senescence and play critical roles in the salt and drought stress response.

scholarly journals Histone Deacetylase Gene SlHDA3 Involves in ABA, Drought and Salt Response in Tomato 

2021 ◽  
Author(s):  
Jun-E Guo
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Histone Deacetylase ◽  
Stress Responses ◽  
Abiotic Stress Tolerance ◽  
Histone Deacetylation ◽  
New Members ◽  
Modifying Factors ◽  
Mda Content

Abstract Histone deacetylation, one of vital modifying factors of post-translation modifications, which is catalyzed by histone deacetylase. The genes of histone deacetylase(HDACs) play critical roles in various stress responses. However, detailed functions for most SlHDAC members in tomato still unknown. In this work, we found that a histone deacetylase, SlHDA3, involved in response to NaCl and drought abiotic stresses. The expression of SlHDA3 was also induced significantly by NaCl, drought stress and endogenous hormone treatments. Silencing of SlHDA3 in tomato, the RNAi transgenic plants presented depressed tolerance to drought and salt stresses compared with WT tomato. The results of sensitivity analysis indicated that the length of hypocotyl and roots in RNAi plants were more inhibited by ABA and salt stress than that of WT at post-germination stage. Worse growth status were exhibited in SlHDA3 transgenic plants under salt and drought stress as are evaluated by a series of physiological parameters related to stress responses, such as decreased RWC, survival rate, ABA content, chlorophyll content and CAT activity, and increased MDA content and proline content. Besides, the expressions analysis of transgenic plants showed that the transcripts of genes which associated with responses to abiotic stress were down-regulated under salt-stressed conditions. To sum up, SlHDA3 acts as a stress-responsive gene, plays a role in the positive regulation of abiotic stress tolerance, and may be one of the new members in the engineering breeding of salt- and drought-tolerant tomato.

Genome-wide identification of citrus calmodulin-like genes and their expression in response to arbuscular mycorrhizal fungi colonization and drought

2021 ◽  
Author(s):  
Bo Shu ◽  
YaChao Xie ◽  
Fei Zhang ◽  
Dejian Zhang ◽  
Chunyan Liu ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Arbuscular Mycorrhizal Fungi ◽  
Stress Responses ◽  
Mycorrhizal Fungi ◽  
Expression Patterns ◽  
Arbuscular Mycorrhizal ◽  
Biotic And Abiotic Stress ◽  
Genome Wide ◽  
A Genome

Calmodulin-like (CML) proteins represent a diverse family of protein in plants, and play significant roles in biotic and abiotic stress responses. However, the involvement of citrus CMLs in plant responses to drought stress (abiotic stress) and arbuscular mycorrhizal fungi (AMF) colonization remain relatively unknown. We characterized the citrus CML genes by analyzing the EF-hand domains and a genome-wide search, and identified a total of 38 such genes, distributed across at least nine chromosomes. Six tandem duplication clusters were observed in the CsCMLs, and 12 CsCMLs exhibited syntenic relationships with Arabidopsis thaliana CMLs. Gene expression analysis showed that 29 CsCMLs were expressed in the roots, and exhibited differential expression patterns. The regulation of CsCMLs expression was not consistent with the cis-elements identified in their promoters. CsCML2, 3, and 5 were upregulated in response to drought stress, and AMF colonization repressed the expression of CsCML7, 9, 12, 13,20, 27, 28, and 35,and induced that of CsCML1, 2, 3, 5, 8, 10, 11, 14, 15, 16, 18, 25, 30, 33, and 37. Furthermore, AMF colonization and drought stress exerted a synergistic effect, evident from the enhanced repression of CsCML7, 9, 12, 13, 27, 28, and 35 and enhanced expression of CsCML2, 3, and 5 under AMF colonization and drought stress. The present study provides valuable insights into the CsCML gene family and its responses to AMF colonization and drought stress.

scholarly journals Overexpression of the ThTPS gene enhanced T. hispida salt and drought stress tolerance

2020 ◽  
Author(s):  
PeiLong Wang ◽  
XiaoJin Lei ◽  
JiaXin Lv ◽  
caiqiu gao
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Expression Patterns ◽  
Evolutionary Relationship ◽  
Drought Stress Tolerance ◽  
Trehalose Metabolism ◽  
Physiological Indexes ◽  
Salt And Drought Stress

Abstract Background: Trehalose is a nonreducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses. Trehalose-6-phosphate synthase (TPS) plays important roles in trehalose metabolism and signaling. Results: A full-length cDNA of ThTPS was cloned from Tamarix hispida. The phylogenetic tree among ThTPS and 11 AtTPS in Arabidopsis indicates that the ThTPS protein had a close evolutionary relationship with AtTPS7. However, the function of AtTPS7 has not been determined. To analyze the abiotic stress tolerance function of ThTPS, the expression patterns of ThTPS were monitored under salt and drought stress and JA, ABA and GA3 hormone stimulation in T. hispida by qRT-PCR. The results showed that ThTPS expression was clearly induced by these 5 kinds of treatments at at least one studied point. Particularly under salt stress, ThTPS was highly induced in the roots of T. hispda. Furthermore, the ThTPS gene was transiently overexpressed in T. hispida. The results of physiological indexes and staining showed that overexpression of the ThTPS gene increased T. hispida salt and drought stress tolerance. Conclusion: The ThTPS gene can respond to abiotic stress such as salt and drought, and overexpression of ThTPS gene can significantly improve salt and drought tolerance. These findings establish a foundation to better understand the response of TPS genes to abiotic stress in plants.

scholarly journals The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jiabin Shen ◽  
Bo Lv ◽  
Liqiong Luo ◽  
Jianmei He ◽  
Chanjuan Mao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Responses ◽  
Environmental Changes ◽  
Abiotic Stress Tolerance ◽  
Late Embryogenesis Abundant ◽  
High Salt ◽  
Marker Genes ◽  
Drought Conditions

Abstract Plants can perceive environmental changes and respond to external stressors. Here, we show that OsNAC2, a member of the NAC transcription factor family, was strongly induced by ABA and osmotic stressors such as drought and high salt. With reduced yields under drought conditions at the flowering stage, OsNAC2 overexpression lines had lower resistance to high salt and drought conditions. RNAi plants showed enhanced tolerance to high salinity and drought stress at both the vegetative and flowering stages. Furthermore, RNAi plants had improved yields after drought stress. A microarray assay indicated that many ABA-dependent stress-related genes were down-regulated in OsNAC2 overexpression lines. We further confirmed that OsNAC2 directly binds the promoters of LATE EMBRYOGENESIS ABUNDANT 3 (OsLEA3) and Stress-Activated Protein Kinases 1 (OsSAPK1), two marker genes in the abiotic stress and ABA response pathways, respectively. Our results suggest that in rice OsNAC2 regulates both abiotic stress responses and ABA-mediated responses, and acts at the junction between the ABA and abiotic stress pathways.

scholarly journals Salt and Drought Stress Responses in Cultivated Beets (Beta vulgaris L.) and Wild Beet (Beta maritima L.)

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1843
Author(s):  
Seher Yolcu ◽  
Hemasundar Alavilli ◽  
Pushpalatha Ganesh ◽  
Madhusmita Panigrahy ◽  
Kihwan Song
Keyword(s):  
Drought Stress ◽  
Beta Vulgaris ◽  
Stress Responses ◽  
Abiotic Stress Tolerance ◽  
Significant Drop ◽  
Drought Tolerant ◽  
Beta Maritima ◽  
Stress Coping Strategies ◽  
Salt And Drought Stress ◽  
Wild Beet

Cultivated beets, including leaf beets, garden beets, fodder beets, and sugar beets, which belong to the species Beta vulgaris L., are economically important edible crops that have been originated from a halophytic wild ancestor, Beta maritima L. (sea beet or wild beet). Salt and drought are major abiotic stresses, which limit crop growth and production and have been most studied in beets compared to other environmental stresses. Characteristically, beets are salt- and drought-tolerant crops; however, prolonged and persistent exposure to salt and drought stress results in a significant drop in beet productivity and yield. Hence, to harness the best benefits of beet cultivation, knowledge of stress-coping strategies, and stress-tolerant beet varieties, are prerequisites. In the current review, we have summarized morpho-physiological, biochemical, and molecular responses of sugar beet, fodder beet, red beet, chard (B. vulgaris L.), and their ancestor, wild beet (B. maritima L.) under salt and drought stresses. We have also described the beet genes and noncoding RNAs previously reported for their roles in salt and drought response/tolerance. The plant biologists and breeders can potentiate the utilization of these resources as prospective targets for developing crops with abiotic stress tolerance.

scholarly journals Genome-wide identification and characterization of TIFY family genes in Moso Bamboo (Phyllostachys edulis) and expression profiling analysis under dehydration and cold stresses

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2620 ◽  
Author(s):  
Zhuo Huang ◽  
Si-Han Jin ◽  
Han-Du Guo ◽  
Xiao-Juan Zhong ◽  
Jiao He ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Abiotic Stress Tolerance ◽  
Divergence Time ◽  
Purifying Selection ◽  
Moso Bamboo ◽  
Whole Genome Sequence ◽  
Evolutionary Analysis ◽  
Phyllostachys Edulis ◽  
Genome Wide

The proteins containing the TIFY domain belong to a plant-specific family of putative transcription factors and could be divided into four subfamilies: ZML, TIFY, PPD and JAZ. They not only function as key regulators of jasmonate hormonal response, but are also involved in responding to abiotic stress. In this study, we identified 24 TIFY genes (PeTIFYs) in Moso bamboo (Phyllostachys edulis) of Poaceae by analyzing the whole genome sequence. OnePeTIFYbelongs to TIFY subfamily, 18 and five belong to JAZ and ZML subfamilies, respectively. Two equivocal gene models were re-predicted and a putative retrotransposition event was found in a ZML protein. The distribution and conservation of domain or motif, and gene structure were also analyzed. Phylogenetic analysis with TIFY proteins ofArabidopsisandOryza sativaindicated that JAZ subfamily could be further divided to four groups. Evolutionary analysis revealed intragenomic duplication and orthologous relationship betweenP. edulis,O. sativa, andB. distachyon. Calculation of the non-synonymous (Ka) and synonymous (Ks) substitution rates and their ratios indicated that the duplication ofPeTIFYmay have occurred around 16.7 million years ago (MYA), the divergence time of TIFY family among theP. edulis-O. sativa,P. edulis-B. distachyon,andO. sativa-B. distachyonwas approximately 39 MYA, 39 MYA, and 45 MYA, respectively. They appear to have undergone extensive purifying selection during evolution. Transcriptome sequencing revealed that more than 50% ofPeTIFYgenes could be up-regulated by cold and dehydration stresses, and somePeTIFYsalso share homology to know TIFYs involved in abiotic stress tolerance. Our results made insights into TIFY family of Moso bamboo, an economically important non-timber forest resource, and provided candidates for further identification of genes involved in regulating responses to abiotic stress.

scholarly journals Balancing trade-offs between biotic and abiotic stress responses through leaf age-dependent variation in stress hormone cross-talk

2019 ◽  
Vol 116 (6) ◽  
pp. 2364-2373 ◽  
Author(s):  
Matthias L. Berens ◽  
Katarzyna W. Wolinska ◽  
Stijn Spaepen ◽  
Jörg Ziegler ◽  
Tatsuya Nobori ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Abiotic Stress Tolerance ◽  
Leaf Age ◽  
Biotic And Abiotic Stress ◽  
Biotic And Abiotic Stresses ◽  
Trade Offs ◽  
Age Dependent

In nature, plants must respond to multiple stresses simultaneously, which likely demands cross-talk between stress-response pathways to minimize fitness costs. Here we provide genetic evidence that biotic and abiotic stress responses are differentially prioritized inArabidopsis thalianaleaves of different ages to maintain growth and reproduction under combined biotic and abiotic stresses. Abiotic stresses, such as high salinity and drought, blunted immune responses in older rosette leaves through the phytohormone abscisic acid signaling, whereas this antagonistic effect was blocked in younger rosette leaves byPBS3, a signaling component of the defense phytohormone salicylic acid. Plants lackingPBS3exhibited enhanced abiotic stress tolerance at the cost of decreased fitness under combined biotic and abiotic stresses. Together with this role,PBS3is also indispensable for the establishment of salt stress- and leaf age-dependent phyllosphere bacterial communities. Collectively, our work reveals a mechanism that balances trade-offs upon conflicting stresses at the organism level and identifies a genetic intersection among plant immunity, leaf microbiota, and abiotic stress tolerance.

scholarly journals The bZIP Transcription Factor GmbZIP15 Negatively Regulates Salt- and Drought-Stress Responses in Soybean

2020 ◽  
Vol 21 (20) ◽  
pp. 7778
Author(s):  
Man Zhang ◽  
Yanhui Liu ◽  
Hanyang Cai ◽  
Mingliang Guo ◽  
Mengnan Chai ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Responses ◽  
Negative Regulator ◽  
Bzip Transcription Factor ◽  
Antioxidant Enzyme Activities ◽  
Oilseed Crop ◽  
Abiotic Stress Response ◽  
Salt And Drought Stress

Soybean (Glycine max), as an important oilseed crop, is constantly threatened by abiotic stress, including that caused by salinity and drought. bZIP transcription factors (TFs) are one of the largest TF families and have been shown to be associated with various environmental-stress tolerances among species; however, their function in abiotic-stress response in soybean remains poorly understood. Here, we characterized the roles of soybean transcription factor GmbZIP15 in response to abiotic stresses. The transcript level of GmbZIP15 was suppressed under salt- and drought-stress conditions. Overexpression of GmbZIP15 in soybean resulted in hypersensitivity to abiotic stress compared with wild-type (WT) plants, which was associated with lower transcript levels of stress-responsive genes involved in both abscisic acid (ABA)-dependent and ABA-independent pathways, defective stomatal aperture regulation, and reduced antioxidant enzyme activities. Furthermore, plants expressing a functional repressor form of GmbZIP15 exhibited drought-stress resistance similar to WT. RNA-seq and qRT-PCR analyses revealed that GmbZIP15 positively regulates GmSAHH1 expression and negatively regulates GmWRKY12 and GmABF1 expression in response to abiotic stress. Overall, these data indicate that GmbZIP15 functions as a negative regulator in response to salt and drought stresses.

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