scholarly journals Molecular cloning and expression analysis of tyrosinases (tyr) in four shell-color strains of Manila clam Ruditapes philippinarum

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8641 ◽  
Author(s):  
Kunyin Jiang ◽  
Liwen Jiang ◽  
Hongtao Nie ◽  
Zhongming Huo ◽  
Xiwu Yan
Keyword(s):  
Developmental Stages ◽  
Larval Growth ◽  
Negative Control ◽  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Cloning And Expression ◽  
Expression Level ◽  
Shell Color ◽  
Color Formation ◽  
Color Strains

The Manila clam (Ruditapes philippinarum) is an economically important molluscan bivalve with variation in pigmentation frequently observed in the shell. In nature, tyrosinase is widely distributed in invertebrates and vertebrates, and plays a crucial role in a variety of physiological activities. In this study, a tyrosinase gene (tyr 9) was cloned and the expression level of tyr genes (tyr 6, tyr 9, tyr 10, and tyr 11) were investigated in different shell colors. Quantitative real-time PCR showed that tyr genes were significantly expressed in the mantle, a shell formation and pigmentation-related tissue. Moreover, the expression pattern of the tyr genes in the mantle of different shell-color strains was different, suggesting that tyrosinases might be involved in different shell-color formation. In addition, the expression profile of tyr 6, tyr 9, tyr 10, and tyr 11 genes were detected at different early developmental stages and the expression level varied with embryonic and larval growth. RNA interference (RNAi) results showed that the expression level of tyr 9 in the RNAi group was significantly down-regulated compared to control and negative control groups, indicating that Rptyr 9 might participate in shell-color formation. Our results indicated that tyr genes were likely to play vital roles in the formation of shell and shell-color in R. philippinarum.

scholarly journals Transcriptome analysis reveals the pigmentation related genes in four different shell color strains of the Manila clam Ruditapes philippinarum

Genomics ◽  
2020 ◽  
Vol 112 (2) ◽  
pp. 2011-2020 ◽  
Author(s):  
Hongtao Nie ◽  
Kunyin Jiang ◽  
Liwen Jiang ◽  
Zhongming Huo ◽  
Jianfeng Ding ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Shell Color ◽  
Color Strains

scholarly journals Identification of shell-color-related microRNAs in the Manila clam Ruditapes philippinarum using high-throughput sequencing of small RNA transcriptomes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jianfeng Ding ◽  
Qiang Wen ◽  
Zhongming Huo ◽  
Hongtao Nie ◽  
Yanjie Qin ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
High Throughput ◽  
Small Rna ◽  
High Throughput Sequencing ◽  
Color Polymorphism ◽  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Shell Color ◽  
Color Formation ◽  
Micro Rnas

AbstractShell-color polymorphism is a common phenomenon in several mollusk species and has been associated with thermal capacity, developmental stability, shell strength, and immunity. Shell-color polymorphism has been related to the differential expression of genes in several signal transduction pathways; however, the functions of micro-RNAs (miRNAs) in shell-color formation remain unclear. In the present study, we compared high-quality, small-RNA transcriptomes in three strains of the Manila clam Ruditapes philippinarum with specific shell-color patterns, artificially selected for six generations. Totals of 114 known and 208 novel miRNAs were identified by high-throughput sequencing, of which nine known and one novel miRNA were verified by stem-loop quantitative real time-polymerase chain reaction. Predicted miRNA targets were subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. miR-137 and miR-216b and the Hedgehog signaling pathway and Wnt signaling pathway were identified as being potentially involved in pigment formation and regulation in R. philippinarum. These results may help to clarify the role of miRNAs in shell coloration and shed light on the mechanisms regulating color formation in bivalve shells.

Studies on phenotypic traits of different shell color strains F2 of Manila clam(Ruditapes philippinarum)

2010 ◽  
Vol 34 (6) ◽  
pp. 701-709
Author(s):  
Xi-wu YAN ◽  
Yue-huan ZHANG ◽  
Zhong-ming HUO ◽  
Huan-qiang SUN ◽  
Feng YANG ◽  
...  
Keyword(s):  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Phenotypic Traits ◽  
Shell Color ◽  
Color Strains

Effects of starvation on larval growth, survival, and metamorphosis of Manila clam Ruditapes philippinarum

2009 ◽  
Vol 29 (6) ◽  
pp. 327-334 ◽  
Author(s):  
Xiwu Yan ◽  
Yuehuan Zhang ◽  
Zhongming Huo ◽  
Feng Yang ◽  
Guofan Zhang
Keyword(s):  
Larval Growth ◽  
Ruditapes Philippinarum ◽  
Manila Clam

scholarly journals Physiological and biochemical responses of different shell color strains of Manila clam to low salinity challenges

2020 ◽  
Vol 16 ◽  
pp. 100260 ◽  
Author(s):  
Hongtao Nie ◽  
Kifat Jahan ◽  
Wenlong Zhang ◽  
Zhongming Huo ◽  
Xiwu Yan
Keyword(s):  
Manila Clam ◽  
Shell Color ◽  
Low Salinity ◽  
Biochemical Responses ◽  
Color Strains ◽  
Physiological And Biochemical

Three way crosses between two-band red and white zebra strains of Manila clam, Ruditapes philippinarum

2011 ◽  
Vol 34 (8) ◽  
pp. 1190-1197
Author(s):  
Xi-wu YAN ◽  
Yue-huan ZHANG ◽  
Huan-qiang SUN ◽  
Zhong-ming HUO ◽  
Xin SUN ◽  
...  
Keyword(s):  
Ruditapes Philippinarum ◽  
Manila Clam
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