scholarly journals Proteogenomic analysis of pitaya reveals cold stress-related molecular signature

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8540
Author(s):  
Junliang Zhou ◽  
Zhuang Wang ◽  
Yongya Mao ◽  
Lijuan Wang ◽  
Tujian Xiao ◽  
...  
Keyword(s):  
Low Temperature ◽  
Cold Stress ◽  
Crop Production ◽  
Molecular Signature ◽  
Reference Database ◽  
Label Free ◽  
Tropical Fruit ◽  
Temperature Damage ◽  
Related Proteins ◽  
Differentially Abundant Proteins

Pitayas (Hylocereus spp.) is an attractive, highly nutritious and commercially valuable tropical fruit. However, low-temperature damage limits crop production. Genome of pitaya has not been sequenced yet. In this study, we sequenced the transcriptome of pitaya as the reference and further investigated the proteome under low temperature. By RNAseq technique, approximately 25.3 million reads were obtained, and further trimmed and assembled into 81,252 unigene sequences. The unigenes were searched against UniProt, NR and COGs at NCBI, Pfam, InterPro and Kyoto Encyclopedia of Genes and Genomes (KEGG) database, and 57,905 unigenes were retrieved annotations. Among them, 44,337 coding sequences were predicted by Trandecoder (v2.0.1), which served as the reference database for label-free proteomic analysis study of pitaya. Here, we identified 116 Differentially Abundant Proteins (DAPs) associated with the cold stress in pitaya, of which 18 proteins were up-regulated and 98 proteins were down-regulated. KEGG analysis and other results showed that these DAPs mainly related to chloroplasts and mitochondria metabolism. In summary, chloroplasts and mitochondria metabolism-related proteins may play an important role in response to cold stress in pitayas.

Overexpression of the acyl-lipid Δ12-desaturase gene protects potato plants from low temperature damage

2011 ◽  
Vol 59 (2) ◽  
pp. 103-115 ◽  
Author(s):  
I. Demin ◽  
C. Shimshilashvili ◽  
N. Yur’eva ◽  
N. Naraykina ◽  
I. Goldenkova-Pavlova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Low Temperature ◽  
Cold Stress ◽  
Malonic Dialdehyde ◽  
Plant Leaves ◽  
Desaturase Gene ◽  
Potato Plants ◽  
Acyl Lipid ◽  
Temperature Damage ◽  
Δ12 Desaturase

The responses of plant leaves to chilling were studied in potato (Solanum tuberosum L., cv. Desnitsa) and in its transformants with the native desA gene that encodes the acyl-lipid Δ12-desaturase from the cyanobacterium Synechocystis sp. PCC 6803 and with the hybrid desA gene fused to the reporter gene of thermostable lichenase (licBM3) from Clostridium thermocellum. Cold stress caused a rapid and significant increase in superoxide production and lipid peroxidation (the content of conjugated dienes and malonic dialdehyde) in wild-type plants. By contrast no significant increase was detected in transformed plants under cold stress conditions. This can be attributed to the fact that the overexpression of the acyl-lipid Δ12-desaturase in transformed potato plants promotes fatty acid polyunsaturation and presumably averts the accelerated generation of the superoxide anion, thus suppressing lipid peroxidation under low-temperature stress

scholarly journals Low Temperature Stress Mediates the Antioxidants Pool and Chlorophyll Fluorescence in Vitis vinifera L. Cultivars

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1877
Author(s):  
Mohammad A. Aazami ◽  
Majid Asghari-Aruq ◽  
Mohammad B. Hassanpouraghdam ◽  
Sezai Ercisli ◽  
Mojmir Baron ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Cold Stress ◽  
Temperature Stress ◽  
Early Spring ◽  
Low Temperature Stress ◽  
Rapid Screening ◽  
Vitis Vinifera L ◽  
Temperature Damage ◽  
Photochemical Quantum Yield

Grapes are sensitive to early autumn and spring low temperature damage. The current study aimed to assay the effects of cold stress (+1 °C for 4, 8, and 16 h) on three grapevine cultivars (Ghiziluzum, Khalili, and Perllete). The results showed that cold stress caused significant changes in the antioxidant and biochemicals content in the studied cultivars. Furthermore, examining the chlorophyll fluorescence indices, cold stress caused a significant increase in minimal fluorescence (F0), a decrease in maximal fluorescence (Fm), and the maximum photochemical quantum yield of photosystem II (Fv/Fm) in all cultivars. Among the studied cultivars, ‘Perllete’ had the highest increase in proline content and activity of antioxidant enzymes and also had the lowest accumulation of malondialdehyde, hydrogen peroxide, electrolyte leakage, and F0, as well as less of a decrease in Fm and Fv/Fm, and had a higher tolerance to cold stress than ‘Ghiziluzum’ and ‘Khalili’. ‘Perllete’ and ‘Ghiziluzum’ showed reasonable tolerance to the low temperature stress. ‘Khalili’ was sensitive to the stress. The rapid screening of grapevine cultivars in early spring low temperatures is applicable with the assaying of some biomolecules and chlorophyll fluorescence.

scholarly journals Alleviation of cold stress in vegetable crops

2020 ◽  
pp. 38-44
Author(s):  
Abdul Rasool Atayee ◽  
Mohammad Safar Noori
Keyword(s):  
Low Temperature ◽  
Plant Growth ◽  
Cold Stress ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Crop Production ◽  
Crop Yields ◽  
Membrane Damage ◽  
Vegetable Production ◽  
Vegetable Crops

Low temperature is a major environmental factor that limits crop productivity of plants. Cold stress is a serious threat to the sustainability of crop yields. Low temperature has a huge impact on the survival and geographical distribution of plants. It negatively affects cellular components and metabolism, and temperature extremes impose stresses of variable severity that depend on the intensity and duration of the stress. Low temperature (less than minimum) leads to chlorosis, necrosis, membrane damage, changes in cytoplasm viscosity, and changes in enzyme activities leading to death of plant. Cold stress disrupts the integrity of intracellular organelles, leading to the loss of compartmentalization. It also causes reduction and impairing of photosynthesis, protein assembly and general metabolic processes. Moreover, cold stress during anthesis induces flower dropping, sterility of pollen, pollen tube distortion, ovule abortion and reduced fruit set, which leads to declined growth and lower yield. A number of approaches are being used to mitigate the deleterious effects of cold stress which threatens the successful vegetable crop production, application of plant growth regulators (salicylic acid, abscisic acid, jasmonic acid, Gibberellin and brassinosteroids) and unitization of genetics tools and plant breeding is one of the strategies to alleviate the low temperature stress in vegetable crops. Plant growth regulators play a greater role in improving the cold stress tolerance. In this paper, the effects of cold stress on vegetable growth, productivity and physiological activities were discussed, and some effective techniques for the mitigation of cold stress that help sustainable vegetable production under fluctuating climate is presented.

scholarly journals O-GlcNAcylation protein disruption by Thiamet G promotes changes on the GBM U87-MG cells secretome molecular signature

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Maria Cecilia Oliveira-Nunes ◽  
Glaucia Julião ◽  
Aline Menezes ◽  
Fernanda Mariath ◽  
John A. Hanover ◽  
...  
Keyword(s):  
Experimental Evidence ◽  
Critical Role ◽  
Molecular Signature ◽  
Label Free ◽  
Intercellular Signaling ◽  
Tumor Aggressiveness ◽  
Signaling Axis ◽  
Literature Evidence ◽  
Therapeutic Tools

AbstractGlioblastoma (GBM) is a grade IV glioma highly aggressive and refractory to the therapeutic approaches currently in use. O-GlcNAcylation plays a key role for tumor aggressiveness and progression in different types of cancer; however, experimental evidence of its involvement in GBM are still lacking. Here, we show that O-GlcNAcylation plays a critical role in maintaining the composition of the GBM secretome, whereas inhibition of OGA activity disrupts the intercellular signaling via microvesicles. Using a label-free quantitative proteomics methodology, we identified 51 proteins in the GBM secretome whose abundance was significantly altered by activity inhibition of O-GlcNAcase (iOGA). Among these proteins, we observed that proteins related to proteasome activity and to regulation of immune response in the tumor microenvironment were consistently downregulated in GBM cells upon iOGA. While the proteins IGFBP3, IL-6 and HSPA5 were downregulated in GBM iOGA cells, the protein SQSTM1/p62 was exclusively found in GBM cells under iOGA. These findings were in line with literature evidence on the role of p62/IL-6 signaling axis in suppressing tumor aggressiveness and our experimental evidence showing a decrease in radioresistance potential of these cells. Taken together, our findings provide evidence that OGA activity may regulate the p62 and IL-6 abundance in the GBM secretome. We propose that the assessment of tumor status from the main proteins present in its secretome may contribute to the advancement of diagnostic, prognostic and even therapeutic tools to approach this relevant malignancy.

Approaches to label-free flexible DNA biosensors using low-temperature solution-processed InZnO thin-film transistors

2014 ◽  
Vol 55 ◽  
pp. 99-105 ◽  
Author(s):  
Joohye Jung ◽  
Si Joon Kim ◽  
Keun Woo Lee ◽  
Doo Hyun Yoon ◽  
Yeong-gyu Kim ◽  
...  
Keyword(s):  
Thin Film ◽  
Low Temperature ◽  
Thin Film Transistors ◽  
Label Free ◽  
Dna Biosensors ◽  
Solution Processed ◽  
Temperature Solution

scholarly journals Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259455
Author(s):  
QianQian Zhuang ◽  
Shaopeng Chen ◽  
ZhiXin Jua ◽  
Yue Yao
Keyword(s):  
Signal Transduction ◽  
Low Temperature ◽  
Temperature Tolerance ◽  
Differentially Expressed ◽  
Metabolomic Analysis ◽  
Temperature Changes ◽  
Low Temperature Tolerance ◽  
Content Ratio ◽  
Temperature Damage ◽  
Hosta Ventricosa

Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold-stress tolerance in this species is unclear. A combination of transcriptomic and metabolomic analysis was used to explore the mechanism of low-temperature tolerance in H. ventricosa. A total of 12 059 differentially expressed genes and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low-temperature signal transduction, possibly by transmitting Ca2+ inside and outside the cell through the ion channels on the three cell membranes of COLD, CNGCs and CRLK, H. ventricosa senses temperature changes and stimulates SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL, thus strengthening its low-temperature resistance. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low-temperature tolerance in this species. The plant protects itself from low-temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.When subjected to low-temperature stress, H. ventricosa perceives temperature changes via COLD, CNGCs and CRLK, and protection from low-temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.

scholarly journals Identification of cold stress responsive microRNAs in cold tolerant and susceptible Hemerocallis fulva by high throughput sequencing

2020 ◽  
Author(s):  
Changbing Huang ◽  
Chun Jiang ◽  
limin Jin ◽  
Huanchao Zhang
Keyword(s):  
Low Temperature ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Temperature Stress ◽  
Stress Responses ◽  
Target Genes ◽  
Low Temperature Stress ◽  
Differentially Expressed ◽  
Differentially Expressed Mirnas ◽  
Cold Tolerant

Abstract Background:Hemerocallis fulva is a perennial herb belonging to Hemerocallis of Hemerocallis. Because of the large and bright colors, it is often used as a garden ornamental plant. But most varieties of H. fulva on the market will wither in winter, which will affect their beauty. It is very important to study the effect of low temperature stress on the physiological indexes of H. fulva and understand the cold tolerance of different H. fulva. MiRNA is a kind of endogenous non coding small molecular RNA with length of 21-24nt. It mainly inhibits protein translation by cutting target genes, and plays an important role in the development of organisms, gene expression and biological stress. Low temperature is the main abiotic stress affecting the production of H. fulva in China, which hinders the growth and development of plants. A comprehensive understanding of the expression pattern of microRNA in H. fulva under low temperature stress can improve our understanding of microRNA mediated stress response. Although there are many studies on miRNAs of various plants under cold stress at home and abroad, there are few studies on miRNAs related to cold stress of H. fulva. It is of great significance to explore the cold stress resistant gene resources of H. fulva, especially the identification and functional research of miRNA closely related to cold stress, for the breeding of excellent H. fulva.Results A total of 5619 cold-responsive miRNAs, 315 putative novel and 5 304 conserved miRNAs, were identified from the leaves and roots of two different varieties ‘Jinyan’ (cold-tolerant) and ‘Lucretius ’ (cold-sensitive), which were stressed under -4 oC for 24 h. Twelve conserved and three novel miRNAs (novel-miR10, novel-miR19 and novel-miR48) were differentially expressed in leaves of ‘Jinyan’ under cold stress. Novel-miR19, novel-miR29 and novel-miR30 were up-regulated in roots of ‘Jinyan’ under cold stress. Thirteen and two conserved miRNAs were deferentially expressed in leaves and roots of ‘Lucretius’ after cold stress. The deferentially expressed miRNAs between two cultivars under cold stress include novel miRNAs and the members of the miR156, miR166 and miR319 families. A total of 6 598 target genes for 6 516 known miRNAs and 82 novel miRNAs were predicted by bioinformatic analysis, mainly involved in metabolic processes and stress responses. Ten differentially expressed miRNAs and predicted target genes were confirmed by quantitative reverse transcription PCR(q-PCR), and the expressional changes of target genes were negatively correlated to differentially expressed miRNAs. Our data indicated that some candidate miRNAs (e.g., miR156a-3-p, miR319a, and novel-miR19) may play important roles in plant response to cold stress.Conclusions Our study indicates that some putative target genes and miRNA mediated metabolic processes and stress responses are significant to cold tolerance in H. fulva.

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