scholarly journals Analysis of the genetic diversity of the coastal and island endangered plant species Elaeagnus macrophylla via conserved DNA-derived polymorphism marker

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8498
Author(s):  
Yi Wang ◽  
Yan Ma ◽  
Bingyu Jia ◽  
Qichao Wu ◽  
Dekui Zang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plant Species ◽  
Diversity Index ◽  
Geographic Distance ◽  
Natural Populations ◽  
Geographical Location ◽  
Endangered Plant ◽  
Endangered Plant Species ◽  
Average Percentage ◽  
Nanji Island

The genetic diversity and genetic structure of five natural populations of the island and coastal endangered plant species Elaeagnus macrophylla were analyzed via conserved DNA-derived polymorphism molecular markers. A total of 289 discernible loci were obtained from 102 individuals via fifteen primers, and 100% of the loci were polymorphic. The observed number of alleles was 1.9654, and the effective number of alleles was 1.2604. Nei’s genetic diversity index was 0.1724 on average, and Shannon’s information index was 0.2869, indicating that Elaeagnus macrophylla had lower levels of genetic diversity than those reported for its continental relatives and other continental species. The average percentage of polymorphic loci was 42.1%, and the maximum and minimum were 80.97% and 14.88%, respectively, which were associated with the Nanji Island and Liugong Island populations, respectively. The populations of Elaeagnus macrophylla were highly differentiated. Cluster analysis revealed that the similarity between the tested samples was related to their geographical location, that the samples from the same island tended to cluster together, and that there was no cross-clustering between samples. The Nanji Island and Da Rushan populations differentiated into two subpopulations. Last, we detected no correlation between genetic distance and geographic distance between populations (Pearson’s correlation coefficient r = 0.256579, p-value = 0.8309).

scholarly journals Analysis of genetic diversity in the coastal and island endangered plant Elaeagnus macrophylla by conserved DNA-derived polymorphism markers

2019 ◽  
Author(s):  
Wang Yi ◽  
Yan Ma ◽  
Bingyu Jia ◽  
Qichao Wu ◽  
Dekui Zang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
Geographic Distance ◽  
Geographical Location ◽  
Group Method ◽  
Endangered Plant ◽  
Average Percentage ◽  
Software Analysis ◽  
Coastal Species ◽  
Nanji Island

The genetic diversity and genetic structure of five natural populations of the island and coastal endangered plant Elaeagnus macrophylla were analyzed by conserved DNA-derived polymorphism (CDDP) molecular markers. A total of 289 discernible loci were obtained from 102 individuals using fifteen primers, and 100% of the loci were polymorphic. The observed number of alleles (Na) was 1.9654, the effective number of alleles (Ne) was 1.2604, the average of Nei’s genetic diversity index (H) was 0.1724, and Shannon's information index was 0.2869, indicating that E. macrophylla had levels of genetic diversity lower than those reported for continental relatives and other coastal species. The average percentage of polymorphic loci (PPL) was 42.1%, and the maximum and minimum PPL values were 80.97% and 14.88%, belonging to the Nanji Island (NJD) and Liugong Island (LGD) populations, respectively. Populations of E. macrophylla were highly differentiated (Gst=0.3263). The unweighted pair group method with arithmetic mean (UPGMA) clustering results revealed 5 groups: one group comprising the Liugong Island (LGD) and Da Rushan (DRS) populations, one comprising the Lingshan Island (LSD), Laoshan (LS) and Daguan Island (DGD) populations, one comprising the Putuo Island (PTD), and two groups representing the Nanji Island (NJD) samples, which were differentiated into a northwestern group and a southeastern group. There was no cross-clustering among the samples, and the similarity of the relatives was strictly related to geographical location. R software analysis showed no correlation between genetic distance and geographic distance between populations (r = 0.256579, p = 0.8309).

scholarly journals Analysis of genetic diversity in the coastal and island endangered plant Elaeagnus macrophylla by conserved DNA-derived polymorphism markers

2019 ◽  
Author(s):  
Wang Yi ◽  
Yan Ma ◽  
Bingyu Jia ◽  
Qichao Wu ◽  
Dekui Zang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
Geographic Distance ◽  
Geographical Location ◽  
Group Method ◽  
Endangered Plant ◽  
Average Percentage ◽  
Software Analysis ◽  
Coastal Species ◽  
Nanji Island

The genetic diversity and genetic structure of five natural populations of the island and coastal endangered plant Elaeagnus macrophylla were analyzed by conserved DNA-derived polymorphism (CDDP) molecular markers. A total of 289 discernible loci were obtained from 102 individuals using fifteen primers, and 100% of the loci were polymorphic. The observed number of alleles (Na) was 1.9654, the effective number of alleles (Ne) was 1.2604, the average of Nei’s genetic diversity index (H) was 0.1724, and Shannon's information index was 0.2869, indicating that E. macrophylla had levels of genetic diversity lower than those reported for continental relatives and other coastal species. The average percentage of polymorphic loci (PPL) was 42.1%, and the maximum and minimum PPL values were 80.97% and 14.88%, belonging to the Nanji Island (NJD) and Liugong Island (LGD) populations, respectively. Populations of E. macrophylla were highly differentiated (Gst=0.3263). The unweighted pair group method with arithmetic mean (UPGMA) clustering results revealed 5 groups: one group comprising the Liugong Island (LGD) and Da Rushan (DRS) populations, one comprising the Lingshan Island (LSD), Laoshan (LS) and Daguan Island (DGD) populations, one comprising the Putuo Island (PTD), and two groups representing the Nanji Island (NJD) samples, which were differentiated into a northwestern group and a southeastern group. There was no cross-clustering among the samples, and the similarity of the relatives was strictly related to geographical location. R software analysis showed no correlation between genetic distance and geographic distance between populations (r = 0.256579, p = 0.8309).

scholarly journals Low Divergence Among Natural Populations of Cornus kousa subsp. chinensis Revealed by ISSR Markers

Forests ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1082 ◽  
Author(s):  
Jia-Qiu Yuan ◽  
Qin Fang ◽  
Guo-Hua Liu ◽  
Xiang-Xiang Fu
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
In Situ Conservation ◽  
Geographic Distance ◽  
Natural Populations ◽  
Issr Markers ◽  
Mantel Test ◽  
Distribution Area ◽  
Genetic Characteristics ◽  
Cornus Kousa

Research Highlights: Taking Chinese dogwood (Cornus kousa subsp. chinensis) as an example, the genetic characteristics of natural populations collected from main a distribution area were evaluated using intersimple sequence repeat (ISSR) markers to reveal the genetic basis for further selection and breeding. Background and Objectives: Chinese dogwood is a small understory tree that is widely distributed in China. Chinese dogwood has attracted interest for its potential horticultural and ornamental values, and its natural resource potential urgently needs to be estimated. Materials and Methods: In this study, the genetic diversity of 12 natural populations collected from six provinces containing 223 individuals was evaluated based on ISSR markers. Results: Relatively high levels of genetic diversity were found at both the population and individual levels. The Shannon’s diversity index (I) among individuals (0.504) was higher than that among populations (0.338). Analysis of molecular variance (AMOVA) revealed that genetic variation mainly existed within populations (61.55%) rather than among populations (38.45%). According to the STRUCTURE analysis, 12 populations were assigned to two groups, i.e., the northern and southern ecological regions, which are separated by the Yangtze River. A Mantel test analysis showed that there was no significant correlation between genetic distance and geographic distance. Conclusions: Considering the breeding system of dogwoods, we speculated that the genetic characteristics of the natural populations of this species would be affected by the dispersal mode of its pollens and seeds; additionally, genetic drift could play an important role in its genetic differentiation. In conclusion, in situ conservation is recommended for Chinese dogwood based on our results.

scholarly journals Some features of rare and endangered plant species introduction

2019 ◽  
Vol 16 ◽  
pp. 00008
Author(s):  
Tatyana Elisafenko ◽  
Olga Dorogina
Keyword(s):  
Plant Species ◽  
Molecular Genetic ◽  
Natural Populations ◽  
Limiting Factors ◽  
Optimal Size ◽  
Endangered Plant ◽  
Species Introduction ◽  
Endangered Plant Species ◽  
Rare And Endangered Species ◽  
Rare And Endangered

The article discusses the features and main stages of rare and endangered plant species introduction. It is necessary to be guided by the principles of priority and expediency in the preparatory stage, which includes the analysis of literary data and preliminary studies in nature. The first stage is the primary introduction; it involves a detailed study of natural populations and organizing an introduction site. Based on the obtained results, the acclimatization assessment is determined. The study of biological features including reproduction is carried out at the second stage. The third stage includes investigations of the introduced populations. The phenorhythm stability, limiting factors determining dates of phenophase onset, the introduction population optimal size and number, seed longevity and conditions of their storage, self-maintenance ability are revealed. As a result, it is given the adaptation assessment, and recommendations are developed to save rare and endangered species in nature and under introduction conditions. Additional studies include applying karyological, molecular-genetic, biotechnological techniques, and possibilities to reconstruct natural populations.

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