scholarly journals Identification and evaluation ofLonicera japonicaflos introduced to the Hailuogou area based on ITS sequences and active compounds

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7636
Author(s):  
Haiyan He ◽  
Dan Zhang ◽  
Jianing Gao ◽  
Theis Raaschou Andersen ◽  
Zishen Mou
Keyword(s):  
Cluster Analysis ◽  
Herbal Medicine ◽  
Chlorogenic Acid ◽  
Large Scale ◽  
Its Sequence ◽  
Active Components ◽  
Flower Buds ◽  
Traditional Chinese Herbal Medicine ◽  
Its Sequence Analysis ◽  
Stems And Leaves

Lonicera japonicaflos (LJF), the dried flower buds ofL. japonicaThunb., have been used in traditional Chinese herbal medicine for thousands of years. Recent studies have reported that LJF has many medicinal properties because of its antioxidative, hypoglycemic, hypolipidemic, anti-allergic, anti-inflammatory, and antibacterial effects. LJF is widely used in China in foods and healthcare products, and is contained in more than 30% of current traditional Chinese medicine prescriptions. Because of this, many Chinese villages cultivate LJF instead of traditional crops due to its high commercial value in the herbal medicine market. Since 2005, the flower buds ofL. japonicaare the only original LJF parts considered according to the Chinese Pharmacopoeia of the People’s Republic of China. However, for historical and commercial reasons, some closely related species ofLonicera Linn. continue to be mislabeled and used as LJF. Currently, there are hundreds of commercial varieties of LJF on the market and it is difficult to choose fine LJF varieties to cultivate. In this study, a total of 21 varieties labeled as LJF on the market were planted in the Hailuogou area. In order to choose the optimum variety, internal transcribed spacer (ITS) sequence alignment analysis was used to test whether the 21 varieties were genuine LJF or not. Cluster analysis of active components based on the content of chlorogenic acid and luteoloside in flower buds, stems and leaves was used to evaluate the quality of the varieties. Results demonstrated that four of the varieties wereL. macranthoidesHand.-Mazz., while the other 17 varieties wereL. japonica, and genuine LJF. The ITS sequence analysis was proven to be highly effective in identifying LJF andLoniceraeflos. Among the 17L. japonicavarieties, the amounts of chlorogenic acid and luteoloside in flower buds, stems and leaves were significantly different. Based on the cluster analysis method, the variety H11 was observed to have the highest level of active components, and is therefore recommended for large-scale planting in the Hailuogou area.

scholarly journals The Great Capacity on Promoting Melanogenesis of Three Compatible Components in Vernonia anthelmintica (L.) Willd.

2021 ◽  
Vol 22 (8) ◽  
pp. 4073
Author(s):  
Yifan Lai ◽  
Qingyuan Feng ◽  
Rui Zhang ◽  
Jing Shang ◽  
Hui Zhong
Keyword(s):  
Herbal Medicine ◽  
Caffeic Acid ◽  
Fluorescent Protein ◽  
Traditional Chinese Herbal Medicine ◽  
Expression Of Genes ◽  
Proliferation And Differentiation ◽  
Green Fluorescent ◽  
Vernonia Anthelmintica

To investigate a possible methodology of exploiting herbal medicine and design polytherapy for the treatment of skin depigmentation disorder, we have made use of Vernonia anthelmintica (L.) Willd., a traditional Chinese herbal medicine that has been proven to be effective in treating vitiligo. Here, we report that the extract of Vernonia anthelmintica (L.) Willd. effectively enhances melanogenesis responses in B16F10. In its compound library, we found three ingredients (butin, caffeic acid and luteolin) also have the activity of promoting melanogenesis in vivo and in vitro. They can reduce the accumulation of ROS induced by hydrogen peroxide and inflammatory response induced by sublethal concentrations of copper sulfate in wild type and green fluorescent protein (GFP)-labeled leukocytes zebrafish larvae. The overall objective of the present study aims to identify which compatibility proportions of the medicines may be more effective in promoting pigmentation. We utilized the D-optimal response surface methodology to optimize the ratio among three molecules. Combining three indicators of promoting melanogenesis, anti-inflammatory and antioxidant capacities, we get the best effect of butin, caffeic acid and luteolin at the ratio (butin:caffeic acid:luteolin = 7.38:28.30:64.32) on zebrafish. Moreover, the effect of melanin content recovery in the best combination is stronger than that of the monomer, which suggests that the three compounds have a synergistic effect on inducing melanogenesis. After simply verifying the result, we performed in situ hybridization on whole-mount zebrafish embryos to further explore the effects of multi-drugs combination on the proliferation and differentiation of melanocytes and the expression of genes (tyr, mitfa, dct, kit) related to melanin synthesis. In conclusion, the above three compatible compounds can significantly enhance melanogenesis and improve depigmentation in vivo.

scholarly journals Cross-Scale Precipitation Variability in a Semiarid Catchment Area on the Western Slopes of the Central Andes

2018 ◽  
Vol 57 (3) ◽  
pp. 675-694 ◽  
Author(s):  
Katja Trachte ◽  
Jochen Seidel ◽  
Rafael Figueroa ◽  
Marco Otto ◽  
Joerg Bendix
Keyword(s):  
Cluster Analysis ◽  
Catchment Area ◽  
Altitudinal Gradient ◽  
Large Scale ◽  
Wrf Model ◽  
Correlation Coefficients ◽  
Elevational Gradient ◽  
Central Andes ◽  
Precipitation Variability ◽  
Precipitation Regimes

AbstractSpatiotemporal precipitation patterns were investigated on the western slopes of the central Andes Mountains by applying EOF and cluster analysis as well as the Weather Research and Forecasting (WRF) Model. In the semiarid catchment area in the highlands of Lima, Peru, the precipitation is assumed to be a cross-scale interplay of large-scale dynamics, varying sea surface temperatures (SSTs), and breeze-dominated slope flows. The EOF analysis was used to encompass and elucidate the upper-level circulation patterns dominating the transport of moisture. To delineate local precipitation regimes, a partitioning cluster analysis was carried out, which additionally should illustrate local effects such as the altitudinal gradient of the Andes. The results demonstrated that especially during the transition to the dry season, synoptic-scale circulation aloft controls the precipitation (correlation coefficients between 0.6 and 0.9), whereas in the remaining seasons the slope breezes due to the altitudinal gradient mainly determine the precipitation behavior. Further analysis with regard to the spatiotemporal precipitation variability revealed an inversion of the precipitation distribution along the elevational gradient within the study area, mainly during February (29%) and March (35%), that showed correlations with coastal SST patterns ranging between 0.56 and 0.67. WRF simulations of the underlying mechanisms disclosed that the large-scale circulation influences the thermally induced upslope flows while the strength of southeastern low-level winds related to the coastal SSTs caused a blocking of easterlies in the middle troposphere through a reduced anticyclonic effect. This interplay enables the generation of precipitation in the usually drier environment at lower elevations, which leads to a decrease in rainfall with increasing elevation.

scholarly journals Simultaneous Determination of 5 Active Components of Periploca forrestii Schltr Extract in Rat Plasma by UPLC-MS and Its Application to Pharmacokinetic Studies in Normal and Adjuvant-Induced Arthritis Model Rats

2020 ◽  
Vol 15 (12) ◽  
pp. 1934578X2098143
Author(s):  
Hui Liu ◽  
Hao Chen ◽  
Xiaoli Qin ◽  
Xue Ma ◽  
Zipeng Gong ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Chlorogenic Acid ◽  
Intravenous Administration ◽  
Area Under The Curve ◽  
Pharmacokinetic Studies ◽  
Active Components ◽  
Arthritis Model ◽  
Adjuvant Induced Arthritis ◽  
Neochlorogenic Acid

Periploca forrestii Schltr ( P. forrestii) is a herb used in traditional Chinese medicine for its anti-rheumatoid arthritis effect. The aim of this study was to compare the pharmacokinetic properties of the 5 active components of this plant: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid C, and periplocin between normal rats and adjuvant-induced arthritis model rats. After the intravenous administration (177.78 mg/kg) of P. forrestii extract, samples were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry. Compared with normal rats, the area under the curve [(AUC)(0-t), AUC(0-∞)], mean residence time [(MRT)(0-t), MRT(0-∞)] of neochlorogenic acid-treated rats decreased significantly, and drug clearance (CL) and apparent volume of distribution (V) increased significantly; the V of chlorogenic acid-treated rats decreased significantly, and MRT(0-t) significantly increased; the AUC(0-t) and AUC(0-∞) of cryptochlorogenic acid-treated rats decreased significantly, and CL and V increased significantly; the AUC(0-t) and MRT(0-t) of isochlorogenic acid C-treated rats decreased significantly, and V increased significantly; the AUC(0-t) and AUC(0-∞) of periplocin-treated rats increased significantly, and MRT(0-t), MRT(0-∞), CL, and V decreased significantly in model rats. The disease condition of rheumatoid arthritis in rats had a significant effect on the in vivo pharmacokinetics of P. forrestii after the intravenous administration.

Young People’s Motivational Profiles in Physical Activity: A Cluster Analysis

2001 ◽  
Vol 23 (1) ◽  
pp. 1-22 ◽  
Author(s):  
C.K. John Wang ◽  
Stuart J.H. Biddle
Keyword(s):  
Physical Activity ◽  
Cluster Analysis ◽  
Young People ◽  
Large Scale ◽  
Goal Orientations ◽  
National Sample ◽  
Achievement Goal Orientations ◽  
Athletic Ability ◽  
Self Worth ◽  
And Gender

A great deal has been written about the motivation of young people in physical activity, and the determinants of activity for this age group have been identified as a research priority. Despite this, there are few large-scale studies identifying “types” or “clusters” of young people based on their scores on validated motivation inventories. This study reports the results of a cluster analysis of a large national sample (n = 2,510) of 12- to 15-year-olds using contemporary approaches to physical activity motivation: achievement goal orientations, self-determination theory (including amotivation), the nature of athletic ability beliefs, and perceived competence. Five meaningful clusters were identified reflecting two highly motivated and two less well-motivated clusters, as well as a clearly amotivated cluster. Groupings were validated by investigating differences in physical activity participation and perceptions of physical self-worth. Some clusters reflected age and gender differences. The results provide valuable information for likely strategies to promote physical activity in young people.

Study of the ESI and APCI interfaces for the UPLC–MS/MS analysis of pesticides in traditional Chinese herbal medicine

2013 ◽  
Vol 406 (5) ◽  
pp. 1481-1491 ◽  
Author(s):  
Lina Chen ◽  
Fengrui Song ◽  
Zhiqiang Liu ◽  
Zhong Zheng ◽  
Junpeng Xing ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Ms Analysis

scholarly journals Feature screening in large scale cluster analysis

2017 ◽  
Vol 161 ◽  
pp. 191-212
Author(s):  
Trambak Banerjee ◽  
Gourab Mukherjee ◽  
Peter Radchenko
Keyword(s):  
Cluster Analysis ◽  
Large Scale ◽  
Feature Screening
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