scholarly journals Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7553 ◽  
Author(s):  
Md Javed Foysal ◽  
Thi Thu Thuy Nguyen ◽  
Md Reaz Chaklader ◽  
Muhammad A.B. Siddik ◽  
Chin-Yen Tay ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Clostridium Butyricum ◽  
Innate Immune Responses ◽  
Feeding Trial ◽  
Treatment Groups ◽  
Carbohydrate Degradation ◽  
Vibrio Mimicus ◽  
Increased Activity ◽  
Tail Muscle ◽  
Rrna Sequences

This study aimed to investigate the effects of Clostridium butyricum as a dietary probiotic supplement in fishmeal based diet on growth, gut microbiota and immune performance of marron (Cherax cainii). Marron were randomly distributed into two different treatment groups, control and probiotic fed group. After 42 days of feeding trial, the results revealed a significant (P < 0.05) increase in growth due to increase in number of moults in marron fed probiotics. The probiotic diet also significantly enhanced the total haemocyte counts (THC), lysozyme activity in the haemolymph and protein content of the tail muscle in marron. Compared to control, the 16S rRNA sequences data demonstrated an enrichment of bacterial diversity in the probiotic fed marron where significant increase of Clostridium abundance was observed. The abundance for crayfish pathogen Vibrio and Aeromonas were found to be significantly reduced post feeding with probiotic diet. Predicted metabolic pathway revealed an increased activity for the metabolism and absorption of carbohydrate, degradation of amino acid, fatty acid and toxic compounds, and biosynthesis of secondary metabolites. C. butyricum supplementation also significantly modulated the expression level of immune-responsive genes of marron post challenged with Vibrio mimicus. The overall results suggest that C. butyricum could be used as dietary probiotic supplement in marron aquaculture.

scholarly journals Effects of Clostridium butyricum- and Bacillus spp.-Based Potential Probiotics on the Growth Performance, Intestinal Morphology, Immune Responses, and Caecal Microbiota in Broilers

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 624
Author(s):  
Xinfu Zeng ◽  
Qing Li ◽  
Caimei Yang ◽  
Yang Yu ◽  
Zixian Fu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Short Chain Fatty Acids ◽  
Clostridium Butyricum ◽  
Intestinal Morphology ◽  
Treatment Groups ◽  
Body Weights ◽  
Bacillus Spp

We aimed to investigate the effects of Clostridium butyricum-, Bacillus subtilis-, and Bacillus licheniformis-based potential probiotics on the growth performance, intestinal morphology, immune responses, and caecal short chain fatty acids (SCFAs) and microbial structure in broiler chickens. Three treatment groups containing a total of 1200 one-day-old AA broilers were included: birds fed with a basal diet only (Con), birds fed with added 1010 probiotics cfu/kg (ProL), and birds fed with added 1011 probiotics cfu/kg (ProH). The dietary probiotics significantly improved the final and average body weights and serum immunoglobulins A, M, and Y. The probiotics also enhanced the ileal morphology and improved the caecal acetate, butyrate, and propionate contents. Furthermore, 16S rRNA sequencing revealed that dietary compound probiotics modulated the caecal microflora composition as follows: (1) all birds shared 2794 observed taxonomic units; (2) treatment groups were well separated in the PCA and PCoA analysis; (3) the relative abundance of Parabacteroides, Ruminococcaceae_UCG-014, Barnesiella, Odoribacter, [Eubacterium_coprostanoligenes_group], [Ruminococcus]_torques_group, and Butyricimonas significantly varied between treatments. The compound probiotics improved the growth performance, serum immune responses, the ratio of ileal villus height to crypt depth, and major caecal SCFAs in broiler chickens. The dietary C. butyricum-, B. subtilis-, and B. licheniformis-based probiotics improved overall broiler health and would benefit the poultry industry.

scholarly journals Dietary purslane (Portulaca oleracea L.) promotes the growth performance of broilers by modulation of gut microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cong Wang ◽  
Qing Liu ◽  
Fengchun Ye ◽  
Hongbo Tang ◽  
Yanpeng Xiong ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Basal Diet ◽  
Scientific Basis ◽  
Control Group ◽  
Good Growth ◽  
Feed Conversion ◽  
Illumina Hiseq ◽  
Treatment Groups ◽  
Dietary Treatments

AbstractPurslane is a widespread wild vegetable with both medicinal and edible properties. It is highly appreciated for its high nutritional value and is also considered as a high-quality feed resource for livestock and poultry. In this study, Sanhuang broilers were used to investigate the effect of feeding purslane diets on the growth performance in broilers and their gut microbiota. A total of 48 birds with good growth and uniform weight were selected and randomly allocated to four treatment groups A (control), B, C and D. Dietary treatments were fed with basal diet without purslane and diets containing 1%, 2% and 3% purslane. The 16S rDNA was amplified by PCR and sequenced using the Illumina HiSeq platform to analyze the composition and diversity of gut microbiota in the four sets of samples. The results showed that dietary inclusion of 2% and 3% purslane could significantly improve the growth performance and reduce the feed conversion ratio. Microbial diversity analysis indicated that the composition of gut microbiota of Sanhuang broilers mainly included Gallibacterium, Bacteroides and Escherichia-Shigella, etc. As the content of purslane was increased, the abundance of Lactobacillus increased significantly, and Escherichia-Shigella decreased. LEfSe analysis revealed that Bacteroides_caecigallinarum, Lachnospiraceae, Lactobacillales and Firmicutes had significant differences compared with the control group. PICRUSt analysis revealed bacteria mainly enriched in carbohydrate metabolism pathway due to the additon of purslane in the diet. These results suggest that the addition of purslane to feed could increase the abundance of Lactobacillus in intestine, modulate the environment of gut microbiota and promote the metabolism of carbohydrates to improve its growth performance. This study indicates that the effect of purslane on the growth-promoting performance of broilers might depend on its modulation on gut microbiota, so as to provide a certain scientific basis for the application of purslane in the feed industry.

scholarly journals Effects of graded levels of Tetracin® on physico-chemical and sensory properties of broiler meat 1

2020 ◽  
Vol 44 (2) ◽  
pp. 34-45
Author(s):  
A. O. Akinwumi ◽  
A. A. Odunsi ◽  
A. B. Omojola ◽  
I. O. Olatoye ◽  
T. O. Akande ◽  
...  
Keyword(s):  
Crude Protein ◽  
Chemical Properties ◽  
Cooking Loss ◽  
Broiler Chicks ◽  
Feeding Trial ◽  
Breast Meat ◽  
Broiler Meat ◽  
Treatment Groups ◽  
Physico Chemical ◽  
Veterinary Antibiotic

Tetracin® (a feed grade veterinary antibiotic) was administered at 0, 50, 100, 150 and 200mg/kg to conventional starter and finisher feed for broilers for a period of six weeks before the breast meat were analyzed for physical, chemical and organoleptic properties. One hundred and fifty (2 weeks old) broiler chicks were randomly divided into 10 birds per replicate with 3 replicates per treatment. At the end of the feeding trial, 6 birds per treatment were slaughtered, defeathered, eviscerated and dressed. The breasts cuts were however subjected to laboratory analyses. No statistically significant (P>0.05) differences were observed in drip loss, chilling loss, shear force, ash and moisture contents of broiler meat across the treatment groups. However, the cooking loss and thermal loss progressively increased (P<0.05) with the corresponding increase in Tetracin®. Similarly, WHC was also significantly (P<0.05) influenced with increased inclusion of Tetracin®. Crude protein increased (P<0.05) but ether extract was reduced with inclusion of Tetracin®. Meat without antibiotics was highly rated (P<0.05) for flavour, juiciness and tenderness while colour and general acceptability were not significantly (P>0.05) influenced with or without Tetracin®. Conclusively, the inclusion of Tetracin® improved the chemical properties of broiler meat but the physical properties (cooking loss and WHC), flavour, juiciness and tenderness of the meat were compromised especially when administered above 100mg/kg feed.

Growth performance of weaner rabbits on diets containing varying levels of dried okra leaves (Abelmoschus esculentus)

2020 ◽  
Vol 47 (3) ◽  
pp. 124-131
Author(s):  
A. A. Imade ◽  
C. F. I. Onwuka ◽  
V. A. Jegede ◽  
O. Y. Ayo-Ajasa ◽  
B. B. Sarumi ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Average Weight ◽  
Feeding Trial ◽  
Design Data ◽  
Treatment Groups ◽  
Alternative Feed ◽  
Randomized Design ◽  
Inclusion Level ◽  
Completely Randomized Design ◽  
Dietary Treatments

The need to seek for alternative feed materials with nutritional and medicinal values to support livestock growth necessitated this study. Forty-five unsexed rabbits crosses (Chinchilla × Newzealand white) 5- 6 weeks old with average weight of 720g were used in a 10- week feeding trial to evaluate the performance of growing rabbits fed on varying levels of dried okra leaves. The rabbits were assigned into five treatment groups containing dried okra leaves (DOL) at 0%, 2.5%, 5%, 7.5% and 10% inclusion levels for dietary treatments (T1, T2, T3, T4 and T5) respectively. Each treatment consisted of 9 rabbits and replicated thrice with each replicate consisting of 3 rabbits in a completely randomized design. Data collected were subjected to one-way analysis of variance (ANOVA). Results indicated that daily feed intake was significantly (P<0.05) largest in rabbits fed 5.00% inclusion level of dried okra leaves (84.57g) and least in rabbits fed 0.00% level of inclusion of dried okra leaves (72.12g). Feed efficiency (FE) was poorest (P<0.05) in rabbits fed 10.00% DOL (0.15%) and best (0.20%) in rabbits fed 2.50% dried okra leaves. It was concluded that okra leaves can be included in diets for growing rabbits and 2.50% level of dried okra leaves inclusion is recommended for adoption by farmers to maximize profit.

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