scholarly journals An attention bias test to assess anxiety states in laying hens

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7303 ◽  
Author(s):  
Dana L.M. Campbell ◽  
Peta S. Taylor ◽  
Carlos E. Hernandez ◽  
Mairi Stewart ◽  
Sue Belson ◽  
...  
Keyword(s):  
Open Field ◽  
Saline Solution ◽  
Laying Hens ◽  
Open Field Test ◽  
Alarm Call ◽  
Attention Bias ◽  
Perceived Threat ◽  
Affective States ◽  
Test Environment ◽  
Time Stepping

Fear is a response to a known threat, anxiety is a response to a perceived threat. Both of these affective states can be detrimental to animal welfare in modern housing environments. In comparison to the well-validated tests for assessing fear in laying hens, tests for measuring anxiety are less developed. Perception of a threat can result in an attention bias that may indicate anxious affective states in individual hens following playback of an alarm call. In Experiment 1, an attention bias test was applied to hens that differed in their range access to show that hens that never ranged were more vigilant (stretching of the neck and looking around:P< 0.001) and slower to feed following the second alarm call playback (P= 0.01) compared with hens that ranged daily. All hens showed a reduction in comb temperature following the first alarm call (P< 0.001). In Experiment 2, an open field test was used to determine an effective dose of 2 mg/kg for the anxiogenic drugmeta-Chlorophenylpiperazine (m-CPP) in adult laying hens. Hens dosed with 2 mg/kg showed reduced locomotion compared with a saline solution (P< 0.05). In Experiment 3, 2 mg/kgm-CPP or saline was administered to adult hens previously habituated to the open field arena to pharmacologically validate an attention bias test as a measure of anxiety. Hens dosed withm-CPP were slower to feed (P= 0.02) and faster to vocalize following a second alarm call playback (P= 0.03) but these hens did not exhibit the same vigilance behavior as documented in Experiment 1. Them-CPP hens also spent more time stepping and vocalizing (bothP< 0.001) than the saline hens. An attention bias test could be used to assess anxiety. However, behavioral responses of hens may vary depending on their age or test environment familiarity, thus further refinement of the test is required. In these tests, 2 mg/kg ofm-CPP resulted in motionless behavior when the environment was novel, but more movement and vocalizing when the environment was familiar. The extreme behavioral phenotypes exhibited by individually-tested birds may both be indicators of negative states.

scholarly journals Application of open field, tonic immobility, and attention bias tests to hens with different ranging patterns

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8122 ◽  
Author(s):  
Dana L.M. Campbell ◽  
Emily J. Dickson ◽  
Caroline Lee
Keyword(s):  
Open Field ◽  
Field Test ◽  
Radio Frequency Identification ◽  
Tonic Immobility ◽  
Laying Hens ◽  
Open Field Test ◽  
Alarm Call ◽  
Attention Bias ◽  
Food Motivation ◽  
Negative Consequences

Assessment of negative affective states is a key component of animal welfare research. In laying hens, excessive fearfulness results in reduced production and increased sensitivity to stress. Fearfulness can be defined as a response to a known threat, but anxiety is a response to an unknown threat and may have similar negative consequences. The open field test and tonic immobility test are commonly applied to measure fearfulness in laying hens. An attention bias test that measured individual hen’s responses to playback of a conspecific alarm call in the presence of food was recently pharmacologically validated using an anxiogenic drug but was confounded by the hen’s typical motionless response in a novel environment. The current study used 56-week old free-range layers to further assess the validity of an attention bias test to differentiate ranging treatment groups in comparison with the open field and tonic immobility tests. The selected hens varied in their range use patterns as tracked by radio-frequency identification technology. ‘Indoor’ hens did not access the range and ‘outdoor’ hens ranged daily; previous research has confirmed higher fearfulness in hens that remain indoors. The tonic immobility test did not differentiate ranging groups (P = 0.34), but indoor birds were slower to first step (P = 0.03) and stepped less (P = 0.02) in the open field test. The attention bias test occurred in an isolated wooden box using a conspecific alarm call playback (a threat) and mixed grain (a positive stimulus). The behavioural response of latency to resume eating following playback of the alarm call was measured to differentiate the anxiety states of the indoor and outdoor ranging birds. Before the attention bias test could occur, birds had to be habituated to the test box across three separate 5-minute sessions to increase the willingness to feed within the novel test environment. All birds ate faster across time (P < 0.001) but the indoor birds were slower to eat than the outdoor birds (P < 0.001). In this study, the latency to resume eating following an alarm call was determined to be a poor measure for highly anxious birds as they failed to eat at all. Forty-six percent of indoor hens were excluded for not eating across the 5-minute test. Of the birds that did eat, only 7% of indoor hens ate following playback of the alarm call, compared with 36% of outdoor hens. This repetition of an attention bias test for laying hens highlights the challenges in assessing hens with extreme fearful/anxious responses and that information may be missed when non-performing hens are excluded from behavioural tests. We suggest that latency to eat in a novel arena without any alarm call playback is an informative measure of anxious state that can be applied to all hens but consideration must be made of potential differences in food motivation.

scholarly journals Toxicity and Neuropharmacological Effects of Elenine

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Eduardo Navarro ◽  
S. J. Alonso ◽  
R. Navarro
Keyword(s):  
Open Field ◽  
Field Test ◽  
Chemical Structure ◽  
Open Field Test ◽  
Control Group ◽  
General Behaviour ◽  
Similar Chemical ◽  
Sedative Effects ◽  
Traction Test ◽  
Central Depressant

Elenine is the aglycone of elenoside, a cytotoxic arylnaphthalene lignan (NSC 644013-W/1) derived fromJusticia hyssopifolia. (Family: Acanthaceae). Elenoside is a β-D-glucoside, with a similar chemical structure to etoposide, exhibiting central depressant activity. In the present study, elenine was given to mice and rats at doses of 10, 20, and 40 mg/kg. Acute toxicity (24 h) and general behaviour in mice was studied as well as its effects on muscular relaxant activity, locomotor activity (Varimex test), and the open-field test and were compared with 10 mg/kg of chlorpromazine. Elenine produced a reduction in the permanence time in muscular relaxant activity (traction test). Spontaneous activity was lower in the Varimex test. The ambulation and rearing were lower compared with the control group, and an increase in boluses was observed in the open-field test. Thus, it can be concluded that elenine has central sedative effects at lower doses than those used with elenoside and has a possible application in conditions of anxiety.

scholarly journals Participation ofGABAAChloride Channels in the Anxiolytic-Like Effects of a Fatty Acid Mixture

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Juan Francisco Rodríguez-Landa ◽  
Rosa Isela García-Ríos ◽  
Jonathan Cueto-Escobedo ◽  
Blandina Bernal-Morales ◽  
Carlos M. Contreras
Keyword(s):  
Open Field ◽  
Field Test ◽  
Chloride Channels ◽  
Wistar Rats ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Acid Mixture ◽  
Plus Maze ◽  
Gaba Binding ◽  
Defensive Burying

Human amniotic fluid and a mixture of eight fatty acids (FAT-M) identified in this maternal fluid (C12:0, lauric acid, 0.9 μg%; C14:0, myristic acid, 6.9 μg%; C16:0, palmitic acid, 35.3 μg%; C16:1, palmitoleic acid, 16.4 μg%; C18:0, stearic acid, 8.5 μg%; C18:1cis, oleic acid, 18.4 μg%; C18:1trans, elaidic acid, 3.5 μg%; C18:2, linoleic acid, 10.1 μg%) produce anxiolytic-like effects that are comparable to diazepam in Wistar rats, suggesting the involvement ofγ-aminobutyric acid-A (GABAA) receptors, a possibility not yet explored. Wistar rats were subjected to the defensive burying test, elevated plus maze, and open field test. In different groups, threeGABAAreceptor antagonists were administered 30 min before FAT-M administration, including the competitive GABA binding antagonist bicuculline (1 mg/kg),GABAAbenzodiazepine antagonist flumazenil (5 mg/kg), and noncompetitiveGABAAchloride channel antagonist picrotoxin (1 mg/kg). The FAT-M exerted anxiolytic-like effects in the defensive burying test and elevated plus maze, without affecting locomotor activity in the open field test. TheGABAAantagonists alone did not produce significant changes in the behavioral tests. Picrotoxin but not bicuculline or flumazenil blocked the anxiolytic-like effect of the FAT-M. Based on the specific blocking action of picrotoxin on the effects of the FAT-M, we conclude that the FAT-M exerted its anxiolytic-like effects throughGABAAreceptor chloride channels.

Acute effects of ethanol on behavioral responses of male and female zebrafish in the open field test with the influence of a non-familiar object

2021 ◽  
pp. 104474
Author(s):  
Thiele P. Souza ◽  
Francini Franscescon ◽  
Flavia V. Stefanello ◽  
Talise E. Müller ◽  
Laura W. Santos ◽  
...  
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Behavioral Responses ◽  
Familiar Object ◽  
Acute Effects ◽  
Male And Female ◽  
Female Zebrafish

Garcinia Camobogia Extract Alters Anxiety, Sociability, and Dopamine Turnover in Male Swiss Albino Mice

2021 ◽  
Author(s):  
Michael K. Ibrahim ◽  
Marina Aboelsaad ◽  
Fatma Tony ◽  
Moustafa Sayed
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Modern Medicine ◽  
Receptor Expression ◽  
Dopamine Turnover ◽  
Male Mice ◽  
Garcinia Cambogia ◽  
Swiss Albino Mice ◽  
Albino Mice

Abstract Background: Obesity is a global concern, closely allied with somatic and psychosomatic disorders. Herbal drugs are available in modern medicine to treat obesity. Garcinia camobogia being used by so many people trying to lose weight produces various systemic side effects. The study was conducted to assess its effect on anxiety, sociability, and dopamine turnover in male mice. Methodology: Male Swiss albino mice of either were divided into three groups with seven mice in each group. Different groups were given distilled water (0.5ml p.o.) and Garcinia cambogia extract at two different doses (100mg/kg and 500 mg/kg p.o.). Effect of test drugs on anxiety was evaluated using open field test. Sociability and social novelty were evaluated using three chambers test. Results (mean ± standard deviation) were analyzed using one-way ANOVA test followed by Tukey’s test. Result: Garcinia cambogia extract significantly increased the time spent in the corners in the open field test, significantly reduced sociability and social novelty in the three chamber test, significantly reduced dopamine turnover and increased D2 receptor expression in ventral tegmental area. Conclusion: Garcinia cambogia extract have significant anxiogenic effect along with reduced sociability and social novelty in male mice. Moreover, these effects could be related to the altered dopamine turnover and D2 receptor expression in mice brain.

Early deprivation increases high-leaning behavior, a novel anxiety-like behavior, in the open field test in rats

2017 ◽  
Vol 123 ◽  
pp. 27-35 ◽  
Author(s):  
Hiroshi Kuniishi ◽  
Satoshi Ichisaka ◽  
Miki Yamamoto ◽  
Natsuko Ikubo ◽  
Sae Matsuda ◽  
...  
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Early Deprivation

scholarly journals A comparative analysis of anxiolytic activity of Arnica montana and alprazolam in rats using open field test

2018 ◽  
Vol 7 (4) ◽  
pp. 718
Author(s):  
Puja Jha ◽  
Seema Bhalerao ◽  
Mrunal Dhole
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Physical Dependence ◽  
Forced Swim Test ◽  
Anxiolytic Activity ◽  
Combination Group ◽  
Control Group ◽  
Arnica Montana ◽  
Group I

Background: Anxiety affects around 7.3% of the total population worldwide. Benzodiazepines are preferred anxiolytic agents and are still frequently used in spite of the side effect profile including muscle relaxation, memory disturbances, sedation, physical dependence. Arnica montana, a traditional herb is known to possess significant anxiolytic effect at the dose of 100mg/kg. In this study, Arnica montana has been compared for the first time with alprazolam, a most commonly used anxiolytic drug.Methods: Forced swim test was used to induce anxiety. Anxiolytic action of study drugs which were given orally, was evaluated using Open field test (OFT) in healthy wistar rats of either sex. Behavior of rats, locomotion and number of squares crossed was recorded. Rats were divided into four groups with eight rats in each group. Study groups were Group I Control; Group II Alprazolam 0.08mg/kg; Group III Arnica montana extract (AME) 100mg/kg; Group IV AME + Alprazolam group 100mg/kg+0.08mg/kg. Statistical analysis was done using ANOVA followed by Tukey’s test (p<0.05).Results: Increase in frequency of rearing was significant (p<0.05) in AME group and highly significant (p<0.001) in Alprazolam and combination group in comparison to control. Decrease in frequency of grooming was highly significant (p<0.001) in Alprazolam and combination group. AME also showed significant (p<0.05) decrease in grooming activity.Conclusions: Arnica montana extract showed anxiolytic activity and can be used as an add on drug after further studies and validation in the treatment of anxiety disorders.

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